Anti-Tyrosine Hydroxylase抗体(ab6211)


  • 产品名称
    Anti-Tyrosine Hydroxylase抗体
    参阅全部 Tyrosine Hydroxylase 一抗
  • 描述
    兔多克隆抗体to Tyrosine Hydroxylase
  • 宿主
  • 特异性
    Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.
  • 经测试应用
    适用于: IHC-FoFr, ELISA, WB, ICC/IF, IHC (PFA fixed), IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Human
    预测可用于: Chicken, Chimpanzee, Macaque monkey
  • 免疫原

    Synthetic peptide corresponding to Rat Tyrosine Hydroxylase aa 32-47 conjugated to Keyhole Limpet Haemocyanin (KLH) (Glutaraldehyde).


    Database link: P04177

  • 阳性对照
    • Mouse or rat brain containing dopaminergic neurons. Ventral mesencephalic neurons (mouse embryonic day 14).
  • 常规说明


    This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.



Our Abpromise guarantee covers the use of ab6211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/500. PubMed: 19630993
ELISA Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa.
ICC/IF 1/1000.
IHC (PFA fixed) 1/1000.
IHC-Fr Use at an assay dependent concentration.


  • 功能
    Plays an important role in the physiology of adrenergic neurons.
  • 组织特异性
    Mainly expressed in the brain and adrenal glands.
  • 通路
    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 疾病相关
    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 序列相似性
    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all


  • Tyrosine hydroxylase antibody (ab6211) staining rat mesencephalic serotoninergic neurons. Pictures taken using objectives X20 (A) or X20 (B). Note that the staining is observed in the cell body and the processes of these neurons. ab6211 was used at 1/1000 incubated for 2 days at room temperature. Secondary antibody was anti-rabbit Alexa Fluor 488 used at 1/1000 for 2 hours at room temperature. Rat brain tissue (ab29475) was perfusion fixed (paraformaldehyde 4% ) followed by post-fixation overnight in the same fixative, cryoprotected in 20% sucrose and then frozen in OCT. 30µm coronal sections of brain were cutting by cyrostat and immunohistochemistry was performed as the 'free floating' technique.

  • ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • Anti-Tyrosine Hydroxylase antibody (ab6211) at 1/100 dilution + PC12 cell lysates at 6 µg

    Performed under reducing conditions.

    Predicted band size: 42,53,84 kDa

    Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.

  • ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.
  • Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.


This product has been referenced in:
  • Zhang X  et al. Autonomic reinnervation and functional regeneration in autologous transplanted submandibular glands in patients with severe keratoconjunctivitis sicca. Int J Oral Sci 10:14 (2018). Read more (PubMed: 29695713) »
  • Ogundele OM  et al. Stress-altered synaptic plasticity and DAMP signaling in the hippocampus-PFC axis; elucidating the significance of IGF-1/IGF-1R/CaMKIIa expression in neural changes associated with a prolonged exposure therapy. Neuroscience 353:147-165 (2017). IHC-Fr ; Rat . Read more (PubMed: 28438613) »

See all 21 Publications for this product


Immunohistochemistry (Resin sections)
Mouse Tissue sections (Retina)

Abcam user community

Verified customer

提交于 Jun 27 2016

Thank you for contacting Abcam.

I apologize for the delay, our laboratory was looking into the availability of the blocking peptide for ab6211.

We do not have the blocking peptide for ab6211 available separately. However, the peptide ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Human Tissue sections (Brain-SNpc)
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Apr 13 2012

Thank you for confirming this information and for your help and cooperation with this case. As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order. As usual ...

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Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem. I have looked through your protocol, which looks fine to me. I a...

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Thank you for your enquiry. Since you are seeing some specific staining in addition to this ubiquitous background staining, I am thinking this may be non-specific staining due to using too high a concentration of primary antibody. The recommendatio...

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Thank you for your enquiry. Regarding "using protein in the phenol-ethanol supernatant for ELISA", would you first precipitate the protein? In either case, I'm not sure if the protein would be recognized by the antibodies since we have not tested t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Rat Tissue sections (Rat mesencephalic brain)
Rat mesencephalic brain

Dr. Sophie Pezet

Verified customer

提交于 Apr 12 2006

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soona s any new information is brought to our attention. As far as we are aware, cross reactivity with human has...

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There are no preservatives present in the whole serum. The whole antiserum was just sterilized by pushing it through a 0.2 um amicon filter.

1-10 of 11 Abreviews or Q&A