Key features and details
- Rabbit polyclonal to Tyrosine Hydroxylase
- Suitable for: IHC-FoFr, WB, ICC/IF, IHC-Fr
- Reacts with: Mouse, Rat, Guinea pig, Human
- Isotype: IgG
参阅全部 Tyrosine Hydroxylase 一抗
描述兔多克隆抗体to Tyrosine Hydroxylase
特异性Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.
经测试应用适用于: IHC-FoFr, WB, ICC/IF, IHC-Frmore details
种属反应性与反应: Mouse, Rat, Guinea pig, Human
预测可用于: Chicken, Chimpanzee, Macaque monkey
This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituent: Whole serum
Concentration information loading...
Primary antibody说明This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
Our Abpromise guarantee covers the use of ab6211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
功能Plays an important role in the physiology of adrenergic neurons.
组织特异性Mainly expressed in the brain and adrenal glands.
通路Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
疾病相关Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
序列相似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
- Information by UniProt
- Dystonia 14 antibody
- DYT14 antibody
- DYT5b antibody
Tyrosine hydroxylase antibody (ab6211) staining rat mesencephalic serotoninergic neurons. Pictures taken using objectives X20 (A) or X20 (B). Note that the staining is observed in the cell body and the processes of these neurons. ab6211 was used at 1/1000 incubated for 2 days at room temperature. Secondary antibody was anti-rabbit Alexa Fluor 488 used at 1/1000 for 2 hours at room temperature. Rat brain tissue (ab29475) was perfusion fixed (paraformaldehyde 4% ) followed by post-fixation overnight in the same fixative, cryoprotected in 20% sucrose and then frozen in OCT. 30µm coronal sections of brain were cutting by cyrostat and immunohistochemistry was performed as the 'free floating' technique.
ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Anti-Tyrosine Hydroxylase antibody (ab6211) at 1/100 dilution + PC12 cell lysates at 6 µg
Performed under reducing conditions.
Predicted band size: 42,53,84 kDa
Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.
ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.
Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
ab6211 被引用在 41 文献中.
- Yang SY et al. Glucocerebrosidase activity, cathepsin D and monomeric a-synuclein interactions in a stem cell derived neuronal model of a PD associated GBA1 mutation. Neurobiol Dis 134:104620 (2020). PubMed: 31634558
- Liu AQ et al. Sensory nerve-deficient microenvironment impairs tooth homeostasis by inducing apoptosis of dental pulp stem cells. Cell Prolif 53:e12803 (2020). PubMed: 32246537
- Rosas-Jarquín CJ et al. Chronic consumption of cassava juice induces cellular stress in rat substantia nigra. Iran J Basic Med Sci 23:93-101 (2020). PubMed: 32405352
- Han X et al. Small molecule-driven NLRP3 inflammation inhibition via interplay between ubiquitination and autophagy: implications for Parkinson disease. Autophagy 15:1860-1881 (2019). PubMed: 30966861
- Lohoff FW et al. Deletion of the vesicular monoamine transporter 1 (vmat1/slc18a1) gene affects dopamine signaling. Brain Res N/A:N/A (2019). PubMed: 30685272