为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Tags & Cell Markers Cell Type Markers Tumor Associated

Anti-Tyrosinase抗体[T311] (ab738)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (11)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Key features and details

  • Mouse monoclonal [T311] to Tyrosinase
  • Reacts with: Human
  • Isotype: IgG2a

你可能会感兴趣

蛋白
Product image
Recombinant Human Tyrosinase protein (Tagged) (ab152776)
二抗
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

查看更多相关产品

概述

  • 产品名称

    Anti-Tyrosinase抗体[T311]
    参阅全部 Tyrosinase 一抗
  • 描述

    小鼠单克隆抗体[T311] to Tyrosinase
  • 宿主

    Mouse
  • 特异性

    Studies have shown tyrosinase to be a very specific marker for melanomas, not cross reacting with any other tumors or normal tissues tested Other studies have shown tyrosinase to be a more sensitive marker when compared to HMB-45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB-45. However, both tyrosinase and MART-1 negative staining was seen in those variants without an epidermal component. Unlike HMB-45, neither tyrosinase or MART-1 discriminates between activated or resting melanocytes. In conclusion, tyrosinase appears to be a superior melanoma marker when compared to HMB-45.
  • 种属反应性

    与反应: Human
  • 免疫原

    Recombinant full length protein.

  • 阳性对照

    • Melanoma
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on [20/07/17]. The following lots are from ascites and are still in stock as of [20/07/17] – [GR278593]. Lot numbers higher than [GR278593] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.099% Sodium azide
    Constituents: 0.9% Proprietary component, 99% Water
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    T311
  • 骨髓瘤

    unknown
  • 同种型

    IgG2a
  • 轻链类型

    kappa
  • 研究领域

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Assay kits

    • Tyrosinase Inhibitor Assay Kit (Colorimetric) (ab204715)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Human Tyrosinase protein (Tagged) (ab152776)

靶标

  • 功能

    This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
  • 疾病相关

    Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
    Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
  • 序列相似性

    Belongs to the tyrosinase family.
  • 细胞定位

    Melanosome membrane.
  • Target information above from: UniProt accession P14679 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7299 Human
    • Omim: 606933 Human
    • SwissProt: P14679 Human
    • Unigene: 503555 Human
    • 别名

      • ATN antibody
      • CMM8 antibody
      • LB24 AB antibody
      • LB24-AB antibody
      • Monophenol monooxygenase antibody
      • OCA1 antibody
      • OCA1A antibody
      • OCAIA antibody
      • Oculocutaneous albinism IA antibody
      • SHEP3 antibody
      • SK29 AB antibody
      • SK29-AB antibody
      • Tumor rejection antigen AB antibody
      • TYR antibody
      • TYRO_HUMAN antibody
      • tyrosinase (oculocutaneous albinism IA) antibody
      • Tyrosinase antibody
      see all

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (11)

    发表研究结果有使用 ab738?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab738 被引用在 11 文献中.

    • Du R & Lei T Effects of autologous platelet-rich plasma injections on facial skin rejuvenation. Exp Ther Med 19:3024-3030 (2020). PubMed: 32256789
    • Kawakami T  et al. Approach for the Derivation of Melanocytes from Induced Pluripotent Stem Cells. J Invest Dermatol 138:150-158 (2018). PubMed: 28887108
    • Margaryan NV  et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci 17:418 (2016). WB . PubMed: 27011171
    • Allouche J  et al. In vitro modeling of hyperpigmentation associated to neurofibromatosis type 1 using melanocytes derived from human embryonic stem cells. Proc Natl Acad Sci U S A 112:9034-9 (2015). Human . PubMed: 26150484
    • Loubéry S  et al. Myosin VI regulates actin dynamics and melanosome biogenesis. Traffic 13:665-80 (2012). PubMed: 22321127
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Question

    What is the concentration of lot GR33380?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 17 2012

    Answer

    Thank you for contacting us.

    The IgG concentration of lot GR33380 is 66.7ug/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Jul 17 2012

    Western blot abreview for Anti-Tyrosinase antibody [T311 ]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A375 - melanoma cell line)
    Loading amount
    10000 cells
    Specification
    A375 - melanoma cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 31 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tyrosinase antibody [T311 ]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris - EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from Dako with Tween
    Read More

    Mr. Rudolf Jung

    Verified customer

    提交于 Sep 06 2011

    Question

    I would like to enquire about recombinant controls for antibodies I recently purchased from you. Are there any recombinant controls or alternative controls for western blotting for the following antibodies; ab738 ab22601 ab1872 ab23798

    Read More

    Abcam community

    Verified customer

    Asked on Nov 15 2005

    Answer

    Thank you for your email. Below I have listed some suggestions for controls to use with these antibodies. If you have any additional questions, please contact Ab23798 - We do sell recombinant Il-1 beta, it is catalog numbers ab9617, ab9723, and ab9788. Also, the antibody clearly stains IL1b positive cells in liver, spleen, pituitary and brain of endotoxin treated rats, so those tissues can be used as a positive control as well. Ab1872 - Placenta is recommended as a positive control. Also, caspase 1 is expressed in larger amounts in spleen and lung. Also found in liver, heart, skeletal muscle, kidney and testis. No expression in the brain. Ab738 - Melanoma is recommended as a positive control. Ab22601 - I couldn't find much information except for this: Msx2 mRNA is found expressed at higher levels in immature tumors, including a yolk sac tumor, seminoma, and chroiocarcinoma, and also in various tumors of epithelial origin. The gene expression is frequently activated in carcinoma derived cell lines.

    Read More

    Abcam Scientific Support

    回复于 Nov 15 2005

    Question

    Has the epitope been mapped?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2005

    Answer

    Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

    Read More

    Abcam Scientific Support

    回复于 Mar 11 2005

    Question

    Your spec-sheet for tyrosinase antibody (ab738) does not have any information for western blotting. I would like to know at what size the band comes out, and how much I need to dilute the antibody for western blotting. And what kind of western protocol would you recommend me using, milk based? BSA-based? Or other ? I would appreciate your prompt response.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 24 2005

    Answer

    Thank you for your enquiry regarding ab738 and its use in WB. It has been reported in the literature that this clone T311 works in western however we have not tested it ourselves, I apologise for the inconvenience. We would recommend doing a preliminary experiment to determine the optimal dilution (a range of dilutions should be tried e.g 1:50-1:500) and we usually recommend to try BSA 5% in TBST (1hr, RT or 4C) for blocking. Please do not hesitate to contact us if you need any more advice or information or have any problems with your experiment,

    Read More

    Abcam Scientific Support

    回复于 Feb 27 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有