Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris based pH 9.5
Sample
Human Tissue sections (oral cavity)
Specification
oral cavity
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: cell signaling antibody diluent #8112
Dilution
1/100
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Hydrogen peroxide block was used prior to overnight incubation (5 min).
We used a polymer detection kit Mach4 universial HRP polymer from Biocare medica, l which was used according to the company's instructions. Dako DAB+ was used for developing.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 05 2013