Anti-Twist抗体[Twist2C1a] (ab50887)
Key features and details
- Mouse monoclonal [Twist2C1a] to Twist
- Suitable for: WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-Twist抗体[Twist2C1a]
参阅全部 Twist 一抗 -
描述
小鼠单克隆抗体[Twist2C1a] to Twist -
宿主
Mouse -
特异性
Good controls are NIH3T3 and A2058 cell lysates. Given that the immunogen shares 92% homology with Twist2, it is possible that it may cross-react. Although some customers have obtained good results in IHC, we do not recommend this antibody for use in IHC. -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human Twist (C terminal).
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阳性对照
- WB: Recombinant Human Twist protein (ab132349) can be used as a positive control. A2058, NIH/3T3 and F2408 whole cell lysate. ICC: HeLa cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
Twist2C1a -
同种型
IgG1 -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (9) |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 21 kDa.
Abcam recommends using BSA blocking. |
ICC | (1) |
Use a concentration of 2 - 100 µg/ml.
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说明 |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 21 kDa. Abcam recommends using BSA blocking. |
ICC
Use a concentration of 2 - 100 µg/ml. |
靶标
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功能
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. -
组织特异性
Subset of mesodermal cells. -
疾病相关
Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7291 Human
- Entrez Gene: 22160 Mouse
- Entrez Gene: 85489 Rat
- Omim: 601622 Human
- SwissProt: Q15672 Human
- SwissProt: P26687 Mouse
- Unigene: 66744 Human
- Unigene: 3280 Mouse
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别名
- ACS3 antibody
- B-HLH DNA binding protein antibody
- bHLHa38 antibody
see all
图片
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All lanes : Anti-Twist antibody [Twist2C1a] (ab50887) at 2 µg/ml
Lane 1 : A2058 (Human melanoma cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : F2408 (Rat normal fibroblast cell line) whole cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 21 kDa -
4% paraformaldehyde-fixed, 0.1% Triton-permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Twist using ab50887 at 100 µg/ml in immunocytochemistry.
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All lanes: Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/250 dilution in PBST for 8 hours at 4°C
Lanes 2, 3, 5, 6, 8 & 9: Whole cell lysate of mouse pancreatic tumor
Lanes 1, 4 & 7: Whole cell lysate of Mouse metastatic pancreatic carcinoma
Lysates at 50 µg per lane
Secondary Antibody: An HRP-conjugated sheep anti-mouse IgG polyclonal (1/2000) developed using the ECL technique
Blocking Step: 10% Milk for 1 hour at room temperature -
Immunostaining with anti-Twist1 monoclonal antibody ab50887 (green, 1/50 dilution) of cryosectioned wild type (fl/wt) (B, C, B′, C′) and conditional knockout (CKO) (D, E, D′, E′) mouse embyonic day 11.5 forelimb buds of embryos harvested from mothers injected with tamoxifen at embryonic day 10.5. (C, C′, E, E′) Merged images showing counterstaining with DAPI (blue). (B′–E′) Higher magnification images of the boxed regions in B–E. An AlexaFlour 488 conjugated donkey anti-mouse was used as the secondary antibody
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ab50887 at 1/1 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat anti-mouse IgG at 1/200 dilution.
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Lanes 2-5 : Anti-Twist antibody [Twist2C1a] (ab50887) at 1/50 dilution
Lane 1 : Marker lane
Lane 2 : Hela whole cell lysate, 25µg
Lane 3 : A2058 whole cell lysate, 25µg
Lane 4 : NIH3T3 whole cell lysate, 25µg
Lane 5 : F2408 whole cell lysate, 25µg
Predicted band size: 21 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
SDS-PAGE with 10-20% gradient gel. -
ab50887 staining Twist in a rat choroid plexus cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. A FITC-conjugated Goat anti-mouse IgG polyclonal (1/80) was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (220)
ab50887 被引用在 220 文献中.
- Li J et al. METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner. Cell Death Dis 13:723 (2022). WB ; Human . PubMed: 35985997
- Li L et al. FERMT1 contributes to the migration and invasion of nasopharyngeal carcinoma through epithelial-mesenchymal transition and cell cycle arrest. Cancer Cell Int 22:70 (2022). PubMed: 35144617
- Han DS & Lee EO Sp1 Plays a Key Role in Vasculogenic Mimicry of Human Prostate Cancer Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35163245
- Oliveira-Rizzo C et al. Hsa-miR-183-5p Modulates Cell Adhesion by Repression of ITGB1 Expression in Prostate Cancer. Noncoding RNA 8:N/A (2022). PubMed: 35202085
- Huang CS et al. Long Noncoding RNA LINC02470 Sponges MicroRNA-143-3p and Enhances SMAD3-Mediated Epithelial-to-Mesenchymal Transition to Promote the Aggressive Properties of Bladder Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 35205713