参阅全部 TUG 一抗
描述兔单克隆抗体[EPR8615] to TUG
经测试应用适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt or ICC
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide corresponding to residues in human TUG (Q9BZE9).
- K562, 293T, and SW480 cell lysates for Western Blot and Human colon tissue for IHC-P.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
存放说明Shipped at 4°C. Store at -20°C.
解离常数（KD）KD = 2.77 x 10 -11 M Learn more about KD
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab131237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 60 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
功能Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface.
组织特异性Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.
疾病相关Note=A chromosomal aberration involving ASPSCR1 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with TFE3 forms a ASPSCR1-TFE3 fusion protein.
Note=A chromosomal aberration involving ASPSCR1 has been found in two patients with of papillary renal cell carcinoma. Translocation t(X;17)(p11.2;q25).
序列相似性Contains 1 UBX domain.
- Information by UniProt
- Alveolar soft part sarcoma chromosomal region candidate gene 1 protein antibody
- Alveolar soft part sarcoma chromosome region candidate 1 (human) antibody
- Alveolar soft part sarcoma locus antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TUG knockout HAP1 cell lysate (20 µg)
Lane 3: NIH/3T3 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab131237 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab131237 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131237 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab131237, at 1/100, staining TUG in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.
All lanes : Anti-TUG antibody [EPR8615] (ab131237) at 1/10000 dilution
Lane 1 : K562 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
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ab131237 has not yet been referenced specifically in any publications.