为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Neuroscience Cell Type Marker Neuron marker Soma marker

Anti-Tubulin抗体[YOL1/34] - Microtubule Marker (ab6161)

  • Datasheet
  • SDS
Reviews (21)Q&A (1)References (113)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Flow Cytometry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

Key features and details

  • Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
  • Suitable for: WB, ICC, Flow Cyt
  • Reacts with: Mouse, Rat, Dog, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Alligator
  • Isotype: IgG2a

你可能会感兴趣

二抗
Product image
Goat Anti-Rat IgG H&L (HRP) (ab97057)

查看更多相关产品

概述

  • 产品名称

    Anti-Tubulin抗体[YOL1/34] - Microtubule Marker
    参阅全部 Tubulin 一抗
  • 描述

    大鼠单克隆抗体[YOL1/34] to Tubulin - Microtubule Marker
  • 宿主

    Rat
  • 经测试应用

    适用于: WB, ICC, Flow Cytmore details
  • 种属反应性

    与反应: Mouse, Rat, Dog, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Alligator
    预测可用于: Plants, a wide range of other species, Mammals
  • 免疫原

    Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.

  • 表位

    ab6161 binds to an epitope between amino acids 414 and 422 of alpha tubulin.
  • 阳性对照

    • WB: HeLa and NIH3T3 whole cell lysates and rat brain tissue lysate. Flow Cyt: methanol fixed/tween permeabilised HeLa cells. ICC: HeLa, NIH/3T3 and human macrophage cells.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    We can conjugate this antibody to FITC for you (please see ab150252 for details). This antibody can be used as a Western blotting loading control (Kops et al.) and as a Microtubule Marker.

    Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    IgG fraction
  • Primary antibody说明

    Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.
  • 克隆

    单克隆
  • 克隆编号

    YOL1/34
  • 同种型

    IgG2a
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195883)
    • Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab195884)
    • HRP Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab196583)
    • Anti-Tubulin antibody [YOL1/34] - BSA and Azide free (ab264065)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Related Products

    • Anisomycin, Protein synthesis inhibitor (ab120495)

应用

Our Abpromise guarantee covers the use of ab6161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml.
ICC Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 序列相似性

    Belongs to the tubulin family.
  • 翻译后修饰

    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68363 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10376 Human
    • Entrez Gene: 203068 Human
    • Entrez Gene: 7280 Human
    • Entrez Gene: 22143 Mouse
    • Entrez Gene: 22151 Mouse
    • Entrez Gene: 22153 Mouse
    • Entrez Gene: 500929 Rat
    • Omim: 191130 Human
    • Omim: 602530 Human
    • Omim: 615101 Human
    • SwissProt: P07437 Human
    • SwissProt: P68363 Human
    • SwissProt: Q13885 Human
    • SwissProt: P05213 Mouse
    • SwissProt: Q7TMM9 Mouse
    • SwissProt: Q9D6F9 Mouse
    • SwissProt: Q6P9V9 Rat
    • Unigene: 524390 Human
    • Unigene: 636480 Human
    • Unigene: 654543 Human
    • Unigene: 392113 Mouse
    • Unigene: 422827 Mouse
    • Unigene: 7420 Mouse
    • Unigene: 99661 Rat
    see all
  • 别名

    • Tubulin beta 2b antibody
    • Alpha tubulin antibody
    • Alpha-tubulin ubiquitous antibody
    • beta Ib tubulin antibody
    • CDCBM5 antibody
    • CDCBM6 antibody
    • fd02b12 antibody
    • K ALPHA 1 antibody
    • M40 antibody
    • OK/SW-cl.56 antibody
    • TBA1B_HUMAN antibody
    • TUBA1B antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin alpha 1b antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin beta 1b antibody
    • Tubulin beta 2A antibody
    • tubulin beta 2A class IIa antibody
    • Tubulin beta antibody
    • tubulin beta chain antibody
    • tubulin beta class I antibody
    • tubulin beta-1 chain antibody
    • tubulin beta-2A chain antibody
    • tubulin beta-5 chain antibody
    • Tubulin K-alpha-1 antibody
    • tubulin, alpha, ubiquitous antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta polypeptide 2 antibody
    • tubulin, beta polypeptide antibody
    • tubulin, beta, class IIA antibody
    • wu:fd02b12 antibody
    • zgc:55461 antibody
    see all

图片

  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)Lab

    ab6161 staining Tubulin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6161 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

    Western blot against tubulin with ab6161 at 1/3000.  Secondary Rabbit anti-Rat IgG HRP (ab6734)was used at 1/2000.  Exposure time: 2mins.

    Lane 1: 20µg/lane HeLa (Human) whole cell lysates (ab7898).

    Lane 2: 20µg/lane 3T3 (Mouse) whole cell lysate (ab7901).

    Lane 3: 20µg/lane Rat brain tissue lysate (ab7942).

  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

    Confocal image of  21 day in vitro rat hippocampal neurons, stained with rat monoclonal antibody to Tubulin - Microtubule Marker (ab6161) in green at 1/500 and Microtubule Associated protein 2 in blue.

    This picture was kindly supplied as part of the review submitted by Dr Jonathon Burman.

  • Flow Cytometry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Flow Cytometry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Overlay histogram showing HeLa cells stained with ab6161 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6161, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

    Cultured human macrophages were used with ab6161 at 1/1000 for immunofluorescence. Cells were fixed with cold 2% formaldehyde for 20mins.

    Green staining is Alexa 568, Blue staining is DAPI stain.

    This cell represents a young macrophage, the staining patterns varied as the cells aged in culture.

  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1/2000 dilution

    All lanes : Yeast (Saccharomyces cerevisiae) whole cell extract prepared by bead-beating

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rat antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds

    See Abreview

  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)

    ICC/IF image of ab6161 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab6161, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.

  • Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Western blot - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg

    Secondary
    Rabbit polyclonal to Rat IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 54 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)This image is courtesy of an anonymous Abreview

    ab6161 staining mouse NIH 3T3 fibroblast cells by ICC/IF. Cells were PFA fixed  and permeabilized in 0.2% Triton X-100 prior to blocking in 5% BSA for 45 minutes at RT.  The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour.  An Alexa Fluor® 568 conjugated goat anti-rat antibody, diluted 1/3000, was used as the secondary.

     

    See Abreview

  • Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    Immunocytochemistry - Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161)
    ab6161 staining tubulin HeLa cells treated with anisomycin (ab120495), by ICC/IF. Increase in tubulin expression correlates with increased concentration of anisomycin as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120495 (anisomycin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6161 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (113)

    发表研究结果有使用 ab6161?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6161 被引用在 113 文献中.

    • Nakabayashi Y  et al. An improved functional analysis of linker-mediated complex (iFALC) strategy. Biochem Biophys Res Commun 526:1164-1169 (2020). PubMed: 32327258
    • El-Kadiry AE  et al. A Novel Sulfonyl-Based Small Molecule Exhibiting Anti-cancer Properties. Front Pharmacol 11:237 (2020). PubMed: 32231565
    • Shao LW  et al. Histone deacetylase HDA-1 modulates mitochondrial stress response and longevity. Nat Commun 11:4639 (2020). PubMed: 32934238
    • Disser NP  et al. Insulin-like growth factor 1 signaling in tenocytes is required for adult tendon growth. FASEB J 33:12680-12695 (2019). PubMed: 31536390
    • Fokin Artem I  et al. Centrosome-derived microtubule radial array, PCM-1 protein, and primary cilia formation. Protoplasma 256:1361-1373 (2019). PubMed: 31079229
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 22 Abreviews or Q&A

    Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 09 2019

    Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Heart)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 09 2019

    Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 09 2019

    Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 09 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (NIH-3T3)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    NIH-3T3
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Spencer Goodman

    Verified customer

    提交于 May 23 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Zebrafish Cell (Embryo)
    Permeabilization
    Yes - Methanol
    Specification
    Embryo
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 20 2016

    Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (BY4741)
    Gel Running Conditions
    Reduced Denaturing (4%-15%)
    Loading amount
    10 µg
    Specification
    BY4741
    Blocking step
    Milk as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 10 2015

    Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Leishmania Cell lysate - whole cell (Leishmania)
    Loading amount
    10 µg
    Specification
    Leishmania
    Gel Running Conditions
    Reduced Denaturing (gel 10%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 17 2011

    Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (Non-small cell lung carcinoma)
    Loading amount
    10 µg
    Specification
    Non-small cell lung carcinoma
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 01 2010

    Western blot abreview for Anti-Tubulin antibody [YOL1/34] - Microtubule Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Candida albicans Cell lysate - whole cell (Candida Albicans Whole Cell Prep)
    Loading amount
    5e+007 cells
    Specification
    Candida Albicans Whole Cell Prep
    Gel Running Conditions
    Non-reduced Denaturing (10% SDS PAGE)
    Blocking step
    LI-COR Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 07 2009

    1-10 of 22 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 3
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有