Key features and details
- Rat monoclonal [YOL1/34] to Tubulin - Microtubule Marker
- Suitable for: WB, ICC, Flow Cyt
- Reacts with: Mouse, Rat, Dog, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Alligator
- Isotype: IgG2a
产品名称Anti-Tubulin抗体[YOL1/34] - Microtubule Marker
参阅全部 Tubulin 一抗
描述大鼠单克隆抗体[YOL1/34] to Tubulin - Microtubule Marker
经测试应用适用于: WB, ICC, Flow Cytmore details
种属反应性与反应: Mouse, Rat, Dog, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Alligator
预测可用于: Plants, a wide range of other species, Mammals
Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.
表位ab6161 binds to an epitope between amino acids 414 and 422 of alpha tubulin.
- WB: HeLa and NIH3T3 whole cell lysates and rat brain tissue lysate. Flow Cyt: methanol fixed/tween permeabilised HeLa cells. ICC: HeLa, NIH/3T3 and human macrophage cells.
This antibody clone is manufactured by Abcam.
We can conjugate this antibody to FITC for you (please see ab150252 for details). This antibody can be used as a Western blotting loading control (Kops et al.) and as a Microtubule Marker.
Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
Primary antibody说明Has been used for the selection of specific recombinant antibodies engineered to incorporate its epitope. It is also useful for studying the function of microtubules.
Our Abpromise guarantee covers the use of ab6161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml.|
|ICC||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
序列相似性Belongs to the tubulin family.
翻译后修饰Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
细胞定位Cytoplasm > cytoskeleton.
- Information by UniProt
- Tubulin beta 2b antibody
- Alpha tubulin antibody
- Alpha-tubulin ubiquitous antibody
ab6161 staining Tubulin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6161 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot against tubulin with ab6161 at 1/3000. Secondary Rabbit anti-Rat IgG HRP (ab6734)was used at 1/2000. Exposure time: 2mins.
Lane 1: 20
µg/lane HeLa (Human) whole cell lysates (ab7898).
Lane 2: 20
µg/lane 3T3 (Mouse) whole cell lysate (ab7901).
Lane 3: 20
µg/lane Rat brain tissue lysate (ab7942).
Confocal image of 21 day in vitro rat hippocampal neurons, stained with rat monoclonal antibody to Tubulin - Microtubule Marker (ab6161) in green at 1/500 and Microtubule Associated protein 2 in blue.
This picture was kindly supplied as part of the review submitted by Dr Jonathon Burman.
Overlay histogram showing HeLa cells stained with ab6161 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6161, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Cultured human macrophages were used with ab6161 at 1/1000 for immunofluorescence. Cells were fixed with cold 2% formaldehyde for 20mins.
Green staining is Alexa 568, Blue staining is DAPI stain.
This cell represents a young macrophage, the staining patterns varied as the cells aged in culture.
All lanes : Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1/2000 dilution
All lanes : Yeast (Saccharomyces cerevisiae) whole cell extract prepared by bead-beating
Lysates/proteins at 5 µg per lane.
All lanes : HRP conjugated goat anti-rat antibody
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
ICC/IF image of ab6161 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab6161, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.
Anti-Tubulin antibody [YOL1/34] - Microtubule Marker (ab6161) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg
Rabbit polyclonal to Rat IgG - H&L (HRP) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
ab6161 staining mouse NIH 3T3 fibroblast cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 5% BSA for 45 minutes at RT. The primary antibody was diluted 1/1000 and incubated with the sample for 1 hour. An Alexa Fluor® 568 conjugated goat anti-rat antibody, diluted 1/3000, was used as the secondary.
ab6161 staining tubulin HeLa cells treated with anisomycin (ab120495), by ICC/IF. Increase in tubulin expression correlates with increased concentration of anisomycin as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120495 (anisomycin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6161 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ab6161 被引用在 113 文献中.
- Nakabayashi Y et al. An improved functional analysis of linker-mediated complex (iFALC) strategy. Biochem Biophys Res Commun 526:1164-1169 (2020). PubMed: 32327258
- El-Kadiry AE et al. A Novel Sulfonyl-Based Small Molecule Exhibiting Anti-cancer Properties. Front Pharmacol 11:237 (2020). PubMed: 32231565
- Shao LW et al. Histone deacetylase HDA-1 modulates mitochondrial stress response and longevity. Nat Commun 11:4639 (2020). PubMed: 32934238
- Disser NP et al. Insulin-like growth factor 1 signaling in tenocytes is required for adult tendon growth. FASEB J 33:12680-12695 (2019). PubMed: 31536390
- Fokin Artem I et al. Centrosome-derived microtubule radial array, PCM-1 protein, and primary cilia formation. Protoplasma 256:1361-1373 (2019). PubMed: 31079229