Application
Western blot
Sample
Human Cell lysate - whole cell (lung, pancreatic, placenta)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
lung, pancreatic, placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rat IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 52 kDa
Additional data
Additional Notes
another fantastic antibody from Abcam. We use the same aliquot over and over again without compromising on the signal strength and specificity. I am very pleased!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Sep 07 2021