Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
293T cells
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Lysis buffer
Concentration
10 µl/mg lysate
Western Blot antibody
Dilution
1/1000
Name
Abcam antibody:Anti-Tubulin antibody [YL1/2] - Loading Control
Additional data
Additional Notes
500 ug of whole cell lysates from 293T cells were incubated with 5 ul of antibody for 3 hours at 4 degree. Then, protein A/G magnetic beads were added and incubated at 4 degree for 2 hours.
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提交于 Nov 08 2019