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Isotype/Loading Controls Loading Controls Tubulin

Anti-Tubulin抗体[YL1/2] - Loading Control (ab6160)

  • Datasheet
  • SDS
Reviews (37)Q&A (1)References (430)

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Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
  • Western blot - Anti-Tubulin antibody [YL1/2] (ab6160)
  • Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
  • Flow Cytometry - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

Key features and details

  • Rat monoclonal [YL1/2] to Tubulin - Loading Control
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-Tubulin抗体[YL1/2] - Loading Control
    参阅全部 Tubulin 一抗
  • 描述

    大鼠单克隆抗体[YL1/2] to Tubulin - Loading Control
  • 宿主

    Rat
  • 经测试应用

    适用于: WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, a wide range of other species, Mammals, African green monkey
  • 免疫原

    Full length native protein (purified) corresponding to Saccharomyces cerevisiae Tubulin.

  • 表位

    The YL1/2 monoclonal epitope has been mapped to the last 8 residues (GEEEGEEY) at the carboxy terminus of alpha tubulin when tyrosinated (PubMed IDs: 6415068, 6204858).
  • 阳性对照

    • ICC/IF: HeLa cells. IHC-P: Human colon tissue. WB: HeLa, NIH/3T3, BALB/3T3 and PC-12 whole cell lysate. Flow Cyt: HeLa cells.
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    Protein G purified
  • Primary antibody说明

    This antibody can be used as a loading control on Western blots (Allen et al.) and is not detected by anti-mouse Ig secondaries. It has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe(OH) epitope. Under some circumstances this antibody may cross-react with other protein including E. coli rec A and oxidized actin.
  • 克隆

    单克隆
  • 克隆编号

    YL1/2
  • 同种型

    IgG2a
  • 研究领域

    • Isotype/Loading Controls
    • Loading Controls
    • Tubulin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Microbiology
    • Organism
    • Eukaryotic Microorganism
    • Yeast
    • Saccharomyces spp

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Tubulin antibody [YL1/2] (ab197737)
    • HRP Anti-Tubulin antibody [YL1/2] - Loading Control (ab197740)
    • Anti-Tubulin antibody [YL1/2] - BSA and Azide free (ab264519)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6160于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (16)
1/5000 - 1/10000.
Flow Cyt
Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF (14)
1/1000. (see PMID: 16230461)
IHC-P (2)
Use at an assay dependent concentration.
说明
WB
1/5000 - 1/10000.
Flow Cyt
Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ICC/IF
1/1000. (see PMID: 16230461)
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 序列相似性

    Belongs to the tubulin family.
  • 翻译后修饰

    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68363 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10376 Human
    • Entrez Gene: 203068 Human
    • Entrez Gene: 7280 Human
    • Entrez Gene: 22143 Mouse
    • Entrez Gene: 22151 Mouse
    • Entrez Gene: 22153 Mouse
    • Entrez Gene: 500929 Rat
    • Omim: 191130 Human
    • Omim: 602530 Human
    • Omim: 615101 Human
    • SwissProt: P07437 Human
    • SwissProt: P68363 Human
    • SwissProt: Q13885 Human
    • SwissProt: P05213 Mouse
    • SwissProt: Q7TMM9 Mouse
    • SwissProt: Q9D6F9 Mouse
    • SwissProt: Q6P9V9 Rat
    • Unigene: 524390 Human
    • Unigene: 392113 Mouse
    • Unigene: 99661 Rat
    see all
  • 别名

    • Tubulin beta 2b antibody
    • Alpha tubulin antibody
    • Alpha-tubulin ubiquitous antibody
    • beta Ib tubulin antibody
    • CDCBM5 antibody
    • CDCBM6 antibody
    • fd02b12 antibody
    • K ALPHA 1 antibody
    • M40 antibody
    • OK/SW-cl.56 antibody
    • TBA1B_HUMAN antibody
    • TUBA1B antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin alpha 1b antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin beta 1b antibody
    • Tubulin beta 2A antibody
    • tubulin beta 2A class IIa antibody
    • Tubulin beta antibody
    • tubulin beta chain antibody
    • tubulin beta class I antibody
    • tubulin beta-1 chain antibody
    • tubulin beta-2A chain antibody
    • tubulin beta-5 chain antibody
    • Tubulin K-alpha-1 antibody
    • tubulin, alpha, ubiquitous antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta polypeptide 2 antibody
    • tubulin, beta polypeptide antibody
    • tubulin, beta, class IIA antibody
    • wu:fd02b12 antibody
    • zgc:55461 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
    Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    ICC/IF image of ab6160 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were fixed in 100% methanol 5 minutes, permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab150165 Alexa Fluor® 488 goat anti-rat IgG (H+L) pre-adsorbed, used at a 1/1000 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 minutes).

     

  • Western blot - Anti-Tubulin antibody [YL1/2] (ab6160)
    Western blot - Anti-Tubulin antibody [YL1/2] (ab6160)
    Lanes 1 & 3 : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1/5000 dilution
    Lanes 2 & 4 : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1/10000 dilution

    Lanes 1-2 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
    Lanes 3-4 : BALB/3T3 whole cell lysate (ab7901)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit Anti-Rat IgG H&L (HRP) (ab6734) at 1/2000 dilution

    Predicted band size: 50 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?
    Additional bands at: 17 kDa, 34 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds
  • Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
    Immunocytochemistry/ Immunofluorescence - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)Loison-Robert et al PLoS One. 2018 Jan 25;13(1):e0190014. doi: 10.1371/journal.pone.0190014. eCollection 2018. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Cytoskeleton and major extracellular matrix proteins in human DPSC (Dental pulp stem cell) were analyzed by immunofluorescence.

    Vimentin and tubulin (Panel D, control, E, (BD) and F, (BR)) are shown.

    After 7 days in contact with/without the materials (Biodentine (BD) and Bioroot (BR)), coated coverslip cultures were fixed in PBS (pH 7.4) containing 4% paraformaldehyde/5% sucrose for 10 minutes. For detection of intracellular molecules, the cells on the coverslips were permeabilized using 0.5% Triton X-100. To block background staining, cells were treated with PBS containing 1% BSA/1% glycine at 37°C for 20 minutes. Samples were incubated with the primary antibody at 4°C overnight or at 37°C for 2 hours. For double immunostaining, primary antibodies were incubated as above. Samples were then incubated with the appropriate secondary antibodies at 37°C for 1 hour. Cell nuclei were stained using DAPI.

    Scale Bar: 100 μm.

  • Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
    Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
    All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase Conjugated Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 52 kDa why is the actual band size different from the predicted?
    Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    IHC image of Tubulin staining in human colon formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer pH 6 for 20 minutes. The section was then incubated with ab6160, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Flow Cytometry - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)
    Flow Cytometry - Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160)

    Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab6160 (red line).

    The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6160, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 1 µg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events was performed.

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (430)

发表研究结果有使用 ab6160?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6160 被引用在 430 文献中.

  • Gori I  et al. Mutations in SKI in Shprintzen-Goldberg syndrome lead to attenuated TGF-ß responses through SKI stabilization. Elife 10:N/A (2021). PubMed: 33416497
  • McGinley LM  et al. Magnetic resonance imaging of human neural stem cells in rodent and primate brain. Stem Cells Transl Med 10:83-97 (2021). PubMed: 32841522
  • Su F  et al. Recombinant high-mobility group box 1 induces cardiomyocyte hypertrophy by regulating the 14-3-3?, PI3K and nuclear factor of activated T cells signaling pathways. Mol Med Rep 23:N/A (2021). PubMed: 33495819
  • Pesaola F  et al. The neuronal ceroid lipofuscinosis-related protein CLN8 regulates endo-lysosomal dynamics and dendritic morphology. Biol Cell N/A:N/A (2021). PubMed: 34021618
  • Zhang H  et al. Hepcidin-induced reduction in iron content and PGC-1ß expression negatively regulates osteoclast differentiation to play a protective role in postmenopausal osteoporosis. Aging (Albany NY) 13:11296-11314 (2021). PubMed: 33820875
View all Publications for this product

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1-10 of 38 Abreviews or Q&A

Western blot abreview for Anti-Tubulin antibody [YL1/2] - Loading Control

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (lung, pancreatic, placenta)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
lung, pancreatic, placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Sep 07 2021

Immunoprecipitation abreview for Anti-Tubulin antibody [YL1/2] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (293T cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
293T cells
Read More

Abcam user community

Verified customer

提交于 Nov 08 2019

Western blot abreview for Anti-Tubulin antibody [YL1/2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
293T cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Sep 10 2018

Immunoprecipitation abreview for Anti-Tubulin antibody [YL1/2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (MEF cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
MEF cells
Read More

Abcam user community

Verified customer

提交于 Sep 06 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Tubulin antibody [YL1/2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (MEFs)
Permeabilization
Yes - Digitonin
Specification
MEFs
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 27°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Aug 31 2018

Western blot abreview for Anti-Tubulin antibody [YL1/2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
MEF cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Aug 01 2018

Western blot abreview for Anti-Tubulin antibody [YL1/2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain cortex)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
30 µg
Specification
brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Read More

DR. Alexandre Magno

Verified customer

提交于 Jul 23 2018

Western blot abreview for Anti-Tubulin antibody [YL1/2]

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Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human cerebral organoid)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12.5% gel)
Loading amount
20 µg
Specification
Human cerebral organoid
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 May 30 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Tubulin antibody [YL1/2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Trophoblast giant cells)
Permeabilization
No
Specification
Trophoblast giant cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 20°C
Fixative
Methanol
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MISS. Stephanie Chrysanthou

Verified customer

提交于 Jul 10 2017

Western blot abreview for Anti-Tubulin antibody [YL1/2]

Excellent
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Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (Hippocampus)
Gel Running Conditions
Reduced Denaturing (13)
Loading amount
6 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

DR. Sergi Bayod

Verified customer

提交于 Nov 28 2016

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研究类别(A-Z)
  • 肿瘤研究
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