Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8241] to TUB 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
参阅全部 TUB 1 一抗
描述兔单克隆抗体[EPR8241] to TUB 1
经测试应用适用于: WB, IHC-Pmore details
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide corresponding to residues near the N-terminus of Human TUB 1 (UniProt P50607).
- SH-SY5Y, Saos-2, C6, L6, and Neuro-2a cell lysates; Human astrocytoma tissue
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
解离常数（KD）KD = 5.43 x 10 -11 M Learn more about KD
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab131034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
功能Functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight (By similarity). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages.
序列相似性Belongs to the TUB family.
细胞定位Cytoplasm. Nucleus. Secreted. Cell membrane. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.
- Information by UniProt
- F10B5.4 antibody
- rd5 antibody
- TUB 1 antibody
All lanes : Anti-TUB 1 antibody [EPR8241] (ab131034) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : L6 cell lysate
Lane 5 : Neuro-2a cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labelling TUB 1 with ab131034 antibody at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
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