重组Anti-TTF2抗体[EPR12756] (ab181116)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12756] to TTF2
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TTF2抗体[EPR12756]
参阅全部 TTF2 一抗 -
描述
兔单克隆抗体[EPR12756] to TTF2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Daudi and HeLa cell line lysates; Jurkat cells; Human adenocarcinoma of endometrium tissue; Human pancreas tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12756 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181116于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/60.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/50 - 1/100.
|
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
|
说明 |
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Flow Cyt (Intra)
1/60. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/50 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 130 kDa. |
靶标
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功能
DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing. -
序列相似性
Belongs to the SNF2/RAD54 helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic during interphase. Relocates to the nucleus as cells enter mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 8458 Human
- Entrez Gene: 74044 Mouse
- Entrez Gene: 295324 Rat
- Omim: 604718 Human
- SwissProt: Q9UNY4 Human
- SwissProt: Q5NC05 Mouse
- Unigene: 486818 Human
- Unigene: 270961 Mouse
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别名
- F2 antibody
- Factor 2 antibody
- Factor2 antibody
see all
图片
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All lanes : Anti-TTF2 antibody [EPR12756] (ab181116) at 1/5000 dilution
Lane 1 : Daudi cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : Jurkat cell line lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 130 kDa -
Immunohistochemical analysis of paraffin embedded Human adenocarcinoma of endometrium tissue labeling TTF2 with ab181116 at 1/250. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling TTF2 with ab181116 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of acetone-fixed Jurkat cells labeling TTF2 with ab181116 at 1/100 (green) dilution. Goat anti rabbit IgG (Alexa Fluor® 488) was used for secondary antibody staining at a dilution of 1/200. Counter stain: Dapi (blue)
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TTF2 with ab181116 at 1/60 dilution, followed byGoat anti rabbit IgG (FITC) at 1/150 dilution (pink).Rabbit monoclonal IgG was used as Isotype control (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab181116 尚未被引用在任何文献中。