重组Anti-TTC11/FIS1抗体[EPR8412] (ab156865)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8412] to TTC11/FIS1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TTC11/FIS1抗体[EPR8412]
参阅全部 TTC11/FIS1 一抗 -
描述
兔单克隆抗体[EPR8412] to TTC11/FIS1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Cow -
免疫原
Synthetic peptide within Human TTC11/FIS1. The exact sequence is proprietary.
Database link: Q9Y3D6 -
阳性对照
- IHC: human kidney WB: Jurkat , Raji, HEK-293 cell lysates
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常规说明
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8412 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab156865于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).
|
IHC-P |
1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
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WB
1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa). |
IHC-P
1/8000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission. -
序列相似性
Belongs to the FIS1 family.
Contains 1 TPR repeat. -
结构域
The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L. -
翻译后修饰
Ubiquitinated by MARCH5. -
细胞定位
Mitochondrion outer membrane. Peroxisome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 615565 Cow
- Entrez Gene: 51024 Human
- Omim: 609003 Human
- SwissProt: Q3T0I5 Cow
- SwissProt: Q9Y3D6 Human
- Unigene: 423968 Human
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别名
- 2010003O14Rik antibody
- CGI 135 antibody
- CGI135 antibody
see all
图片
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All lanes : Anti-TTC11/FIS1 antibody [EPR8412] (ab156865) at 1/10000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling TTC11/FIS1 with Purified ab156865 at 1/8000 dilution (0.08 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab156865 被引用在 9 文献中.
- Cai C et al. Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway. Redox Biol 52:102288 (2022). PubMed: 35325804
- Xia R et al. Moxibustion alleviates chronic heart failure by regulating mitochondrial dynamics and inhibiting autophagy. Exp Ther Med 23:359 (2022). PubMed: 35493422
- Su F et al. Mitochondrial BKCa Mediates the Protective Effect of Low-Dose Ethanol Preconditioning on Oxygen-Glucose Deprivation and Reperfusion-Induced Neuronal Apoptosis. Front Physiol 12:719753 (2021). PubMed: 34759831
- Zheng Y et al. The Imbalance of Mitochondrial Fusion/Fission Drives High-Glucose-Induced Vascular Injury. Biomolecules 11:N/A (2021). PubMed: 34944423
- Zhang D et al. Increased mitochondrial fission is critical for hypoxia-induced pancreatic beta cell death. PLoS One 13:e0197266 (2018). PubMed: 29768513
- Kristensen MD et al. Obesity leads to impairments in the morphology and organization of human skeletal muscle lipid droplets and mitochondrial networks, which are resolved with gastric bypass surgery-induced improvements in insulin sensitivity. Acta Physiol (Oxf) N/A:e13100 (2018). PubMed: 29791782
- Bertolin G et al. Aurora kinase A localises to mitochondria to control organelle dynamics and energy production. Elife 7:N/A (2018). PubMed: 30070631
- Huang Q et al. Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways. Autophagy 12:999-1014 (2016). PubMed: 27124102
- Bruns DR et al. Mitochondrial integrity in a neonatal bovine model of right ventricular dysfunction. Am J Physiol Lung Cell Mol Physiol 308:L158-67 (2015). PubMed: 25416385