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Signal Transduction Growth Factors/Hormones Hormones

Anti-TSH beta抗体[11E4] (ab6069)

  • Datasheet
  • SDS
Submit a review Q&A (6)

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Key features and details

  • Mouse monoclonal [11E4] to TSH beta
  • Suitable for: Sandwich ELISA
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-TSH beta抗体[11E4]
    参阅全部 TSH beta 一抗
  • 描述

    小鼠单克隆抗体[11E4] to TSH beta
  • 宿主

    Mouse
  • 特异性

    This antibody specifically recognizes ß-subunit of human TSH.
  • 经测试应用

    适用于: Sandwich ELISAmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human TSH beta. Full length native protein (purified) from human pituitary gland.

  • 常规说明

    This antibody (capture) is ELISA paired with ab6070 (conjugate).

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 纯化说明

    Purity is tested by electrophoresis.
  • 克隆

    单克隆
  • 克隆编号

    11E4
  • 骨髓瘤

    Sp2/0
  • 同种型

    IgG1
  • 研究领域

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Thyroid axis
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6069于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Sandwich ELISA
说明
应用说明
sELISA: Use at an assay dependent dilution.
Can be paired for ELISA with Mouse monoclonal [7G12] to TSH (ab6070).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

靶标

  • 相关性

    Various hormones are secreted from the anterior pituitary during development and growth. Thyroid Stimulating Hormone (TSH) is secreted from the anterior pituitary and controls thyroid structure and metabolism
  • 细胞定位

    Secreted
  • 数据库链接

    • Entrez Gene: 7252 Human
    • Omim: 188540 Human
    • SwissProt: P01222 Human
    • 别名

      • CHNG4 antibody
      • Thyroid stimulating hormone beta subunit antibody
      • Thyroid stimulating hormone, beta precursor antibody
      • Thyrotropin beta subunit antibody
      • TSHB antibody
      see all

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (0)

    发表研究结果有使用 ab6069?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6069 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Question

    Inquiry: Will this TSH protein (Active) (ab110682) work as an antigen with the primary antibodies you sell? Specifically mouse monoclonal anti-human TSH antibodies Ab6069, Ab6071 and Ab24921? Thanks!

    Read More

    Abcam community

    Verified customer

    Asked on May 08 2012

    Answer

    Thank you for your enquiry.

    The TSH protein ab110682 is purified human protein. So in theory it should be detected by any antibody which will cross react withhuman TSH. Although please make sure the antibody is also tested in the application of interest.

    I hope this is helpful. Please contact us again if you have any further questions.

    Read More

    Abcam Scientific Support

    回复于 May 08 2012

    Question

    Quick question that I think I asked you before, but I can't remember what you said. Does Abcam have a "positive control antigen" to test these antibodies with? I am using Thyrotropic hormone from human pituitary from Sigma-Aldrich product number T9265. I contacted Sigma just to make sure there wasn't something weird about this. They did not have specifications on its use in immunoassays and they recommended asking the antibody manufacturer for a positive control. Do you have a TSH antigen that you can recommend?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 04 2012

    Answer

    It is definitely my pleasure to help you with these issues. I look forward to hearing how the new product works in your experiments.

    We do not have the specific antigen used to create these products available on our catalog at this time. However we do offer a full length purified nativeTSH protein which is in its active form. That product number is AB110682 and can be found at the following link:

    https://www.abcam.com/TSH-protein-Active-ab110682.html

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Abcam Scientific Support

    回复于 Apr 04 2012

    Question

    sELISA in mouse and rat for TSH, FSH and Luteinizing Hormone
    pairs:
    Luteinizing Hormone: ab72837 (mouse), ab72838 (mouse) - test in mouse, rat
    FSH: ab21011 (mouse, capture), ab30543 (HRP)- test in sELISA, mouse, rat
    TSH: ab6070 (HRP), ab6069 - test in mouse, rat
    send info on proteins for standard curve and secondary Abs (anti-mouse IgG) if required
    Luteinizing Hormone: none
    FSH: ab51888 (human) - test in sELISA
    TSH: ab110682 (human) - test in sELISA
    ab98799 - donkey anti-mouse IgG, preabsorbed, HRP
    ab98808 - goat anti-mouse IgG, preabsorbed, HRP

    Read More

    Abcam community

    Verified customer

    Asked on Mar 08 2012

    Answer

    Thank you for your phone call. I am sending you the following discount codes for trying the antibodies in sELISA and mouse/rat. As for the proteins, I have found the following:

    Luteinizing Hormone: none
    FSH: ab51888 (human) - test in sELISA
    TSH: ab110682 (human) - test in sELISA

    Since the FSH and TSH proteins have not yet been tested in sELISA, I am sending you also discount codes for these proteins (see below). Unfortunately, we do not carry the Luteinizing Hormone protein.

    In terms of secondary antibodies, you would only need one for the Luteinizing Hormone sELISA as none of these antibodies has an HRP label. The other antibody pairs for TSH and FSH contain an HRP-labeled primary antibody.

    The suggested secondary antibodies are:
    ab98799 - donkey anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98799.html)
    ab98808 - goat anti-mouse IgG, preabsorbed, HRP (https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-HRP-pre-adsorbed-ab98808.html)

    Testing Discount Codes:

    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in sELISA, mouse, rat
    DISCOUNT CODE: xxx-test in sELISA, mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat
    DISCOUNT CODE: xxx- test in mouse, rat

    DISCOUNT CODE: xxx ab51888 (human FSH protein) - test in sELISA
    DISCOUNT CODE: xxx ab110682 (human TSH protein) - test in sELISA

    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test thes antibodies and proteins in sELISA and nouse/rat. Each code will give you 1 free PRIMARY ANTIBODY or PROTEIN before the expiration date. To redeem this offer, please submit each an Abreview for sELISA and mouse/rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Please also remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    回复于 Mar 08 2012

    Question

    Not seeing signal when ab24921 is used to capture TSH from solution. What kind of buffer should be used for the coating and what should the pH be?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2012

    Answer

    Thank you for your call this morning and for your patience.

    There is an error on the pdf form of our sandwich ELISA protocol, and the carbonate buffer should be around 9.6. I've looked through several protocols, and most recommend either a pH 9.6 carbonate buffer or a 7.4 PBS solution for coating. I think that the buffer you are currently using is fine, and I apologize for the mistake on the protocol.

    It's possible that a higher concentration of antibody or antigen will help increase the signal. Could you send me some more details about the protocol and results that you've seen, and I will see if there are any suggestions or explanations?

    I look forward to hearing from you. Please let me know if there is anything else that I can do for you and I'll be happy to help.

    Read More

    Abcam Scientific Support

    回复于 Feb 22 2012

    Question

    What is the ELISA kit that uses this matched pair? How do we convert international units to picograms?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 20 2012

    Answer

    Thank you for contacting us.

    I have have the chance to look over each of the TSH ELISA kits. Unfortunately it appears that none of the currently offered kits uses ab6069 or ab24921. It may be that the particular product that was recommended to you if no longer offered at Abcam.

    Each of our kits is covered by our Abpromise and is 100% guaranteed to work should they be used in a manner consistent with what we have published in the datasheet. However,should you to perform a sandwichELISA using ab6069 I have found that the the matched pair for ab6069 is actually ab6070 and not ab24921. Again, if you decide to purchase this pair for use in sandwich ELISA they will be covered by our Abpromise.

    I was able to find the conversion factor for IU to SI for TSH, that factor is 1.0 and may be found at the following website:

    http://www.questdiagnostics.com/hcp/intguide/SI_Units.pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Jan 20 2012

    Question

    Are these two antibodies cross-reactive with FSH, LH, or HCG?  We were looking for antibodies to the beta subunit, but we may try the ones you suggested if there is no problem with cross-reactivity.  Thank you for checking on it for me!  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2011

    Answer

    Our antibody ab24921 does not react with LH, FSH or HCG, however it is unclear which subunit of the TSH protein this antibody is directed against. On the other hand we do know that ab6069 is specific to the beta subunit of TSH but there is no further information at this time if the antibody shows any cross-reactivity with LH, FSH or HCG.

    Read More

    Abcam Scientific Support

    回复于 Oct 12 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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