概述

  • 产品名称
    Anti-TSG101抗体[4A10]
    参阅全部 TSG101 一抗
  • 描述
    小鼠单克隆抗体[4A10] to TSG101
  • 宿主
    Mouse
  • 特异性
    This antibody recognizes the TSG-101 protein, the product of a recently identified tumor susceptibility gene the inactivation of which in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice.
  • 经测试应用
    适用于: Flow Cyt, ICC/IF, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Drosophila melanogaster
  • 免疫原

    Fusion protein containing amino acids 167-374 of the TSG101 protein expressed in E. coli.

  • 阳性对照
    • MOLT4, HeLa
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 12th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab83 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 23045692

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/100 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/100. Do not perform antigen retrieval.

靶标

  • 功能
    Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
  • 组织特异性
    Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
  • 序列相似性
    Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
    Contains 1 SB (steadiness box) domain.
    Contains 1 UEV (ubiquitin E2 variant) domain.
  • 结构域
    The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
    The coiled coil domain may interact with stathmin.
    The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
  • 翻译后修饰
    Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
  • 细胞定位
    Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • ESCRT I complex subunit TSG101 antibody
    • ESCRT-I complex subunit TSG101 antibody
    • TS101_HUMAN antibody
    • TSG 10 antibody
    • TSG 101 antibody
    • TSG10 antibody
    • Tsg101 antibody
    • Tumor susceptibility 101 antibody
    • Tumor susceptibility gene 10 antibody
    • Tumor susceptibility gene 101 antibody
    • Tumor susceptibility gene 101 protein antibody
    • Tumor susceptibility protein antibody
    • Tumor susceptibility protein isoform 3 antibody
    • VPS 23 antibody
    • VPS23 antibody
    see all

图片

  • Western blot - Anti-TSG101 antibody [4A10] (ab83)
    Western blot - Anti-TSG101 antibody [4A10] (ab83)
    All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1/500 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : K562 whole cell lysate
    Lane 5 : THP-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [4A10] (ab83)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [4A10] (ab83)

    Immunohistochemical analysis of paraffin-embedded colon carcinoma tissue labeling TSG101 with ab83 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [4A10] (ab83)
    Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [4A10] (ab83)Image from Wang G et al., J Biol Chem. 2012 Jun 15;287(25):21384-95. Epub 2012 Apr 24. Fig 7.; doi: 10.1074/jbc.M112.340513; June 15, 2012, The Journal of Biological Chemistry, 287, 21384-21395.
    Immunofluorescence analysis of amyloid peptide-treated mouse astrocytes, staining TSG101 with ab83.

    Astrocytes were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100 for 5 min at room temperature. Cells were incubated with primary antibody and a fluorescent-conjugated anti-mouse IgG was used as the secondary antibody.
  • Flow Cytometry - Anti-TSG101 antibody [4A10] (ab83)
    Flow Cytometry - Anti-TSG101 antibody [4A10] (ab83)Image from Kahlert C et al., J Biol Chem. 2014;289(7):3869-75. Fig 1(C).; doi: 10.1074/jbc.C113.532267.

    Panc-1 and Serum Exosomes were characterized by the exosome-specific expression of TSG101 by FACS analysis.

    Exosomes were attached to 4-μm aldehyde/sulfate latex beads by mixing ∼30 μg of exosomes in a 100-μl volume of beads for 2 hours at room temperature. This suspension was diluted to 1 ml with PBS, and the reaction was stopped with 100 mm glycine and 2% BSA in PBS. Exosome-bound beads were washed in PBS/1% BSA, blocked with 10% BSA, and stained for FACS with anti-TSG101 (1:400, ab83). An Alexa Fluor® 488 conjugated anti-mouse IgG was used as the secondary antibody.

  • Western blot - Anti-TSG101 antibody [4A10] (ab83)
    Western blot - Anti-TSG101 antibody [4A10] (ab83)
    All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 whole cell lysate (ab7909)
    Lane 3 : Jurkat whole cell lysate (ab7899)
    Lane 4 : HEK293 whole cell lysate (ab7902)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Donkey polyclonal to Mouse IgG (IRDyeTM 700DX) at 1/10000 dilution

    Predicted band size: 43 kDa
    Observed band size: 49 kDa (why is the actual band size different from the predicted?)

  • Western blot - Anti-TSG101 antibody [4A10] (ab83)
    Western blot - Anti-TSG101 antibody [4A10] (ab83)
    All lanes : Anti-TSG101 antibody [4A10] (ab83)

    Lane 1 : 20ug HeLa cell extract
    Lane 2 : 20ug HeLa cell extract from cells treated with TSG101 siRNA

    Predicted band size: 43 kDa
    Observed band size: 47 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Cooks T  et al. Mutant p53 cancers reprogram macrophages to tumor supporting macrophages via exosomal miR-1246. Nat Commun 9:771 (2018). Read more (PubMed: 29472616) »
  • Gheinani AH  et al. Improved isolation strategies to increase the yield and purity of human urinary exosomes for biomarker discovery. Sci Rep 8:3945 (2018). Read more (PubMed: 29500443) »

See all 90 Publications for this product

发表研究结果有使用 ab83？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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11 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-TSG101 antibody [4A10]

 Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Hela
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Username

Abcam user community

Verified customer

提交于 Mar 20 2018

You suggested that I shoulduse protease inhibitor in our samples for WB.Wouldpleaseyou give me some tips on what kind I use and what I have to think when Iorder one of those, Inwhich step should Iuses this. another question was about the denaturing of ...

Read More

Thank you for contacting us.

You can use any protease inhibitor, most common are Leupeptin and PMSF. These protease inhibitors should be dissolved in lysis buffer.

People tend to use protease inhibitors cocktails which have many en...

Read More

Our samples are from proteins extracted from human monocytes exosomes. Tsg 101 is works like marker for these exosomes. We were very happy when we saw bands with right size in our samples, but what didn`t make me happy was these extra bands with same s...

Read More

Thank very much for filling the questionnaire and for sending the image.

I have reviewed the protocol now and would like to point out few things that may be the cause of multiple bands;

- Protease inhibitors; You have mentioned that...

Read More

We bought a while ago an antibody anti-TSG101 (4A10) (ab83) from Abcam. We've been running WB and have got the right band with the right size. My question is what are the other bands that we look at this membrane by this antibody. I am sending the pict...

Read More

Thanks you for your email.

There could be many reasons for this extra band. It could be an Isoform; TSG101 have 2 isoforms one is 31kDa nd other is 44 kDa. Two bands could be observed however it will depend on the lysates being used. http://w...

Read More

We ordered ab92726 (CD9), ab41803 (Anxa2), ab83 (Tsg101) from Abcam.

What conditions do you recommend for each one of these Abs for running Westerns – reducing or non-reducing?

Thank you for contacting Abcam.

Forthese antibodies I would recommend using reducing conditions and also initially using a 1/1000 primary antibody dilution, although you may to alter this concentration for subsequent western blots.

...

Read More

Western blot abreview for Anti-TSG101 antibody [4A10]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human HeLa)
Loading amount
30 µg
Specification
Human HeLa
Gel Running Conditions
Reduced Denaturing (12.5% gel)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Nov 25 2009

Western blot abreview for Anti-TSG101 antibody [4A10]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (brain, HEK cell lysate)
Loading amount
50 µg
Specification
brain, HEK cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More
abreview image
Username

Dr. Sriharsha Kantamneni

Verified customer

提交于 Sep 26 2007

I have a customer who has experienced a product shortage. Please refer to his message: We received TSG101 Ab (4A10), ABCAM product through Sapphire in Dec 2005. According to data sheet, we should have had 100ug of the ab, which according to conc s...

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The datasheet was correct and the customer should have received 100ul, I apologise for this problem and have arranged a free of charge vial to be sent to you. Many thanks for your help with this matter,

I had reported that I was not getting any staining with ab83. Rat and mouse tissue. I basically wanted to know: 1.What dilution is suggested. 2.Is this antibody known to work on paraformalin-fixed paraffin embedded tissue. 3.If so, what pretreatm...

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Thank you for your email. I contacted the originator of this antibody who was able to provide me with the following information. "Concerning IHC staining, you may want to refer to the following publication for a detailed protocol: Zhu et at, Oncogene 2...

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I'm adding two coplaint protocols for different applications: I Abcam Troubleshooting form ?Immunohistochemistry- 1. Please describe the problem (high background, no staining etc). weak but overall, unspecific staining of the whole cell. No spe...

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Thank you for the details that you have provided and I'm sorry to hear that your customer is experiencing difficulty with this antibody. You mentioned that a previously purchased batch worked well; do you have that batch number? Were the protocols and ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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