Anti-TSG101抗体[4A10] (ab83)
Key features and details
- Mouse monoclonal [4A10] to TSG101
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-TSG101抗体[4A10]
参阅全部 TSG101 一抗 -
描述
小鼠单克隆抗体[4A10] to TSG101 -
宿主
Mouse -
特异性
This antibody recognizes the TSG-101 protein, the product of a recently identified tumor susceptibility gene the inactivation of which in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice. -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-P HumanWB MouseHuman -
免疫原
Fusion protein corresponding to Human TSG101 aa 167-374. Expressed in E.coli
Database link: Q99816 -
阳性对照
- WB: Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12 , HeLa, HepG2, A431, K562 and THP-1 whole cell lysate, mouse testis. ICC/IF: Amyloid peptide-treated mouse astrocytes IHC-P: human ovarian cancer tissue, human breast carcinoma tissue. Flow cyt: THP-1 cells
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常规说明
This product was changed from ascites to tissue culture supernatant on 12th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS
w/o preservative -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE. -
克隆
单克隆 -
克隆编号
4A10 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab83 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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Flow Cyt |
Human
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IHC-P |
Human
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WB |
Mouse
Human
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应用 | Ab评论 | 说明 |
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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WB | (3) |
1/500 - 1/3000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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WB
1/500 - 1/3000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa). |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. -
组织特异性
Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
Contains 1 SB (steadiness box) domain.
Contains 1 UEV (ubiquitin E2 variant) domain. -
结构域
The UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
The coiled coil domain may interact with stathmin.
The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently. -
翻译后修饰
Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1. -
细胞定位
Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis. - Information by UniProt
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数据库链接
- Entrez Gene: 7251 Human
- Entrez Gene: 22088 Mouse
- Omim: 601387 Human
- SwissProt: Q99816 Human
- SwissProt: Q61187 Mouse
- Unigene: 523512 Human
- Unigene: 241334 Mouse
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别名
- ESCRT I complex subunit TSG101 antibody
- ESCRT-I complex subunit TSG101 antibody
- TS101_HUMAN antibody
see all
图片
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All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1/500 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8D30 whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody at 1/500 dilution
Predicted band size: 43 kDa10% SDS PAGE
Running conditions: 80V for 15min then 140V for 40min
Blocking: 5% non-fat milk in TBST at room temperature for 60min.
Washing conditions: 5 ml TBST, 4 x 5min
Transfer conditions: Semi-dry, 18 V, 60min (NC membrane)
Exposure system: Trident plus Western HRP Substrate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [4A10] (ab83)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue labeling TSG101 with ab83 at 1/100 dilution. Cytoplasmic staining is observed. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H2O2, RT, 30min. Blocking condition: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30min. Primary antibody incubation: 4°C overnight. Secondary antibody incubation: HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 minutes. DAB detection.
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Flow Cytometry - Anti-TSG101 antibody [4A10] (ab83)Image from Kahlert C et al., J Biol Chem. 2014;289(7):3869-75. Fig 1(C).; doi: 10.1074/jbc.C113.532267.
Panc-1 and Serum Exosomes were characterized by the exosome-specific expression of TSG101 by FACS analysis.
Exosomes were attached to 4-μm aldehyde/sulfate latex beads by mixing ∼30 μg of exosomes in a 100-μl volume of beads for 2 hours at room temperature. This suspension was diluted to 1 ml with PBS, and the reaction was stopped with 100 mm glycine and 2% BSA in PBS. Exosome-bound beads were washed in PBS/1% BSA, blocked with 10% BSA, and stained for FACS with anti-TSG101 (1:400, ab83). An Alexa Fluor® 488 conjugated anti-mouse IgG was used as the secondary antibody.
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Flow Cytometry analysis of THP-1 cells labeling TSG101 with ab83 at 1/25 dilution (red). Unlabelled sample was used as a control (black). A Dylight 488-conjugated secondary antibody was used for FACS analysis.
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All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1/500 dilution
Lane 1 : Non-transfected (–) 293T whole cell extracts
Lane 2 : TSG101 shRNA transfected (+) 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody
Developed using the ECL technique.
Predicted band size: 43 kDa10% SDS-PAGE
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All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1/500 dilution
Lane 1 : Mouse testis tissue extract
Lane 2 : Mouse pancreas tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody
Developed using the ECL technique.
Predicted band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [4A10] (ab83)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling TSG101 with ab83 at 1/100 dilution. Cytoplasmic staining is observed. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1/500 dilution
Lane 1 : K562 whole cell extract
Lane 2 : THP-1 whole cell extract
Lane 3 : HL-60 whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody
Developed using the ECL technique.
Predicted band size: 43 kDa10% SDS-PAGE
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All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1/500 dilution
Lane 1 : NIH-3T3 whole cell lysate/extract
Lane 2 : JC (mouse mammary adenocarcinoma) whole cell lysate/extract
Lane 3 : BCL-1 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody
Predicted band size: 43 kDa10% SDS-PAGE
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All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1/500 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : K562 whole cell lysate
Lane 5 : THP-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa -
All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :A-431 whole cell lysate (ab7909)
Lane 3 :Jurkat whole cell lysate (ab7899)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Donkey polyclonal to Mouse IgG (IRDyeTM 700DX) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (168)
ab83 被引用在 168 文献中.
- Chen HX et al. Exosomes derived from mesenchymal stem cells repair a Parkinson's disease model by inducing autophagy. Cell Death Dis 11:288 (2020). PubMed: 32341347
- Liu Y et al. Extracellular vesicle tetraspanin-8 level predicts distant metastasis in non-small cell lung cancer after concurrent chemoradiation. Sci Adv 6:eaaz6162 (2020). PubMed: 32195353
- Castellano JJ et al. Extracellular Vesicle lincRNA-p21 Expression in Tumor-Draining Pulmonary Vein Defines Prognosis in NSCLC and Modulates Endothelial Cell Behavior. Cancers (Basel) 12:N/A (2020). PubMed: 32244977
- Just J et al. Blood flow-restricted resistance exercise alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles. Sci Rep 10:5835 (2020). PubMed: 32245988
- Colangelo NW & Azzam EI Extracellular vesicles originating from glioblastoma cells increase metalloproteinase release by astrocytes: the role of CD147 (EMMPRIN) and ionizing radiation. Cell Commun Signal 18:21 (2020). PubMed: 32033611