Anti-Tryptophan Hydroxylase/TPH (phospho S260)抗体(ab30574)
Key features and details
- Rabbit polyclonal to Tryptophan Hydroxylase/TPH (phospho S260)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Tryptophan Hydroxylase/TPH (phospho S260)抗体
参阅全部 Tryptophan Hydroxylase/TPH 一抗 -
描述
兔多克隆抗体to Tryptophan Hydroxylase/TPH (phospho S260) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to Rat Tryptophan Hydroxylase/TPH (phospho S260).
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阳性对照
- Rat brain stem lysate, human dorsal Raphe nucleus.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Ab30574 was affinity purified via sequential chromatography on phospho and dephosphopeptide affinity columns. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab30574于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
靶标
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组织特异性
Isoform 2 seems to be less widely expressed than isoform 1. -
通路
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. -
序列相似性
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain. - Information by UniProt
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数据库链接
- Entrez Gene: 24848 Rat
- SwissProt: P09810 Rat
- Unigene: 94788 Rat
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别名
- Indoleacetic acid 5 hydroxylase antibody
- L tryptophan hydroxylase antibody
- MGC119994 antibody
see all
图片
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All lanes : Anti-Tryptophan Hydroxylase/TPH (phospho S260) antibody (ab30574) at 1/1000 dilution
Lane 1 : rat brain stem lysate (control)
Lane 2 : rat brain stem lysate, incubated with S260 phosphopeptide (negative control)
Lane 3 : rat brain stem lysate, incubated with corresponding non phosphopeptide.
Lysates/proteins at 15 µg per lane.
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: <43 kDa, 48 kDa, 59 kDa. We are unsure as to the identity of these extra bands.
In lane 2, the labeling is specifically blocked by the S260 phosphopeptide used as antigen.
In lane 3, the corresponding nonphosphopeptide did not block the immunolabeling.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab30574 被引用在 4 文献中.
- Herrera-Martínez AD et al. Effect of the Tryptophan Hydroxylase Inhibitor Telotristat on Growth and Serotonin Secretion in 2D and 3D Cultured Pancreatic Neuroendocrine Tumor Cells. Neuroendocrinology 110:351-363 (2020). PubMed: 31319410
- Damen R et al. The stimulatory adenosine receptor ADORA2B regulates serotonin (5-HT) synthesis and release in oxygen-depleted EC cells in inflammatory bowel disease. PLoS One 8:e62607 (2013). PubMed: 23638125
- Chin A et al. The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion. Am J Physiol Gastrointest Liver Physiol 302:G397-405 (2012). WB ; Human . PubMed: 22038827
- Haugen M et al. Differential signal pathway activation and 5-HT function: the role of gut enterochromaffin cells as oxygen sensors. Am J Physiol Gastrointest Liver Physiol 303:G1164-73 (2012). WB ; Human . PubMed: 22936271