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Neuroscience Neurotransmission Receptors / Channels More Ion Channels

Anti-TRPV1抗体(ab6166)

  • Datasheet
Reviews (2)Q&A (5)References (14)

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Western blot - Anti-TRPV1 antibody (ab6166)
  • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)
  • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)
  • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)

Key features and details

  • Rabbit polyclonal to TRPV1
  • Suitable for: WB, IHC-FrFl
  • Reacts with: Mouse
  • Isotype: IgG

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概述

  • 产品名称

    Anti-TRPV1抗体
    参阅全部 TRPV1 一抗
  • 描述

    兔多克隆抗体to TRPV1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-FrFlmore details
  • 种属反应性

    与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to Rat TRPV1 aa 800-900 (C terminal) conjugated to keyhole limpet haemocyanin (Glutaraldehyde).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    This antibody will stain a selected population of sensory neurons in the dorsal root ganglion. It is unsuitable for staining nerve fibres or terminals.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • More Ion Channels
    • Neuroscience
    • Sensory System
    • Somatosensory system
    • Nociception
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TRPV1 protein (ab159807)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6166于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000. Predicted molecular weight: 94.976 kDa.
IHC-FrFl
1/100 - 1/2000.

Use at 1/500– 1/2000 dilution for DAB staining and 1/100 – 1/500 dilution for immunofluorescence staining.

说明
WB
1/1000. Predicted molecular weight: 94.976 kDa.
IHC-FrFl
1/100 - 1/2000.

Use at 1/500– 1/2000 dilution for DAB staining and 1/100 – 1/500 dilution for immunofluorescence staining.

靶标

  • 功能

    Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • 组织特异性

    Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • 序列相似性

    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • 结构域

    The association domain (AD) is necessary for self-association.
  • 翻译后修饰

    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • 细胞定位

    Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Target information above from: UniProt accession Q8NER1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 193034 Mouse
    • SwissProt: Q704Y3 Mouse
    • Unigene: 447485 Mouse
    • 别名

      • Capsaicin receptor antibody
      • DKFZp434K0220 antibody
      • osm 9 like TRP channel 1 antibody
      • Osm-9-like TRP channel 1 antibody
      • OTRPC1 antibody
      • Transient receptor potential cation channel subfamily V member 1 antibody
      • TRPV 1 antibody
      • Trpv1 antibody
      • TRPV1_HUMAN antibody
      • Vanilloid receptor 1 antibody
      • Vanilloid receptor subtype 1 antibody
      • VR 1 antibody
      • VR1 antibody
      see all

    图片

    • Western blot - Anti-TRPV1 antibody (ab6166)
      Western blot - Anti-TRPV1 antibody (ab6166)
      Anti-TRPV1 antibody (ab6166) at 1/1000 dilution + Mouse dorsal root ganglia at 20 µg

      Predicted band size: 94.976 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)
      Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)

      Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting TRPV1 using ab6166 at a 1/500 dilution. 40x Magnification. Right: Negative control.

    • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)
      Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)

      Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting TRPV1 using ab6166 at a 1/500 dilution. 40x Magnification. Right: Negative control.

    • Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)
      Immunohistochemistry - Free Floating - Anti-TRPV1 antibody (ab6166)

      Immunohistochemistry analysis of 4% paraformaldehyde fixed/free-floating mouse dorsal root ganglia detecting TRPV1 using ab6166 at a 1/500 dilution.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (14)

    发表研究结果有使用 ab6166?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6166 被引用在 14 文献中.

    • Oh S  et al. Radiofrequency Irradiation Modulates TRPV1-Related Burning Sensation in Rosacea. Molecules 26:N/A (2021). PubMed: 33800730
    • Chen Y  et al. Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity. J Inflamm Res 14:325-339 (2021). PubMed: 33584100
    • Zhang Y  et al. The mechanism of Annexin A1 to modulate TRPV1 and nociception in dorsal root ganglion neurons. Cell Biosci 11:167 (2021). PubMed: 34446102
    • Di Y  et al. Ursolic acid protects against cisplatin-induced ototoxicity by inhibiting oxidative stress and TRPV1-mediated Ca2+-signaling. Int J Mol Med 46:806-816 (2020). PubMed: 32626955
    • Xing F  et al. MZF1 in the Dorsal Root Ganglia Contributes to the Development and Maintenance of Neuropathic Pain via Regulation of TRPV1. Neural Plast 2019:2782417 (2019). PubMed: 31582966
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-TRPV1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (dorsal ganglia)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    dorsal ganglia
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
    Read More

    DR. Alexandre Magno

    Verified customer

    提交于 Aug 14 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-TRPV1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Rectum)
    Permeabilization
    Yes - After antigen retrival and a PBS wash (3X), 5% ice cold acetone was applied for 5 minutes, slides were then washed with PBS (3x) and 0.1% Triton was applied for 15 minutes.
    Specification
    Rectum
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Donna Daly

    Verified customer

    提交于 Sep 06 2017

    Question

    thank you so much. We will wainting for a replacement vial of ab6166 in your next shipment.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 21 2012

    Answer

    I'm happy to help! Please let me know if you need anything further.

    Read More

    Abcam Scientific Support

    回复于 Mar 21 2012

    Question

    I confirm that our PO is xxxx (13th of december 2011), and your reference numeber is xxxxx.
    Please let me know is you can send us asap a new vial of ab6166.

    Thanks in advance

    Read More

    Abcam community

    Verified customer

    Asked on Mar 21 2012

    Answer

    Thanks for the additional information.

    I have located the order on our system and have arranged for a replacement vial of ab6166 to be included in Abcam's next shipment to you.

    For your records I have found the Abcam order reference number for that order was xxxx.

    Please let me know if you need anything further.

    Read More

    Abcam Scientific Support

    回复于 Mar 21 2012

    Question

    Dear All,
    please find in attachement a complaint for ab6166.
    Please note that I can't provide you with the lot number because after aliquoting the antibody, the customer threw away the original vial.
    The customer has already used this antibody (another lot, but she doesn't not the lot number), with excellent results.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 20 2012

    Answer

    Thank you for your enquiry.

    I am sorry to hear of your customer's troubles using ab6166. I have read the attached complaint report and it does appear the most recent vial of ab6166 is not working as expected. I would be happy to offer a replacement or credit.

    I have been unsuccessful in locating the order based on the PO. Please double check this number. Thanks!

    Read More

    Abcam Scientific Support

    回复于 Mar 20 2012

    Question

    Dear All,  our customer bought your AB6166 (our PO 3426), but the color of the liquid within the vial is dark red. The lot number is GR21183-1 She has already bought this antibody previously but the color was light yellow. Is it normal? The customer test it and she found a red background in her tissue sections. Please let me know Thanks in advance Kind regards

    Read More

    Abcam community

    Verified customer

    Asked on Jan 19 2012

    Answer

    Thank you for your enquiry and interest. This antibody is not purified and as the datasheet indicates we sell it as whole antiserum. ab6166 is a polyclonal antibody and there is batch-to-batch variation; some bleed may contain higher levels of red blood cells/or breakdown product of hemoglobulins than others. If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Jan 19 2012

    Question

    Is this antibody to the intracellular or extracellular part of the receptor?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 13 2001

    Answer

    This antibody was raised against the intra-cellular C-terminal.

    Read More

    Abcam Scientific Support

    回复于 Feb 15 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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