概述

  • 产品名称
  • 描述
    兔多克隆抗体to TRPM8
  • 宿主
    Rabbit
  • 特异性
    Reacts with residues 278-292 and the C terminus sequence of the human trpm8 protein.
  • 经测试应用
    适用于: ELISA, IP, IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Guinea pig, Human
  • 免疫原

    Synthetic peptide corresponding to Human TRPM8.
    Sequence:

    RNQLEKYISERTIQD Plus NDLKGLLKEIANKIK

  • 阳性对照
    • WB: Transfected COS cell lysate; human liver tissue lysate; human hepatocyte lysate. PMID 17510704, prostate cells used as positive control. PC-3 suitable negative control.

性能

应用

Our Abpromise guarantee covers the use of ab3243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/100 - 1/2000.
IP Use at an assay dependent concentration.

used at 6 ug/mg cell lysate.

IHC-P 1/10 - 1/2000.
WB 1/500 - 1/1000. Predicted molecular weight: 128 kDa.
IHC-Fr 1/100. See Abreview.

靶标

  • 功能
    Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
  • 组织特异性
    Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
  • 序列相似性
    Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Long transient receptor potential channel 6 antibody
    • LTrpC-6 antibody
    • LTrpC6 antibody
    • MGC2849 antibody
    • Short form of the TRPM8 cationic channel antibody
    • Transient receptor potential cation channel subfamily M member 8 antibody
    • Transient receptor potential p8 antibody
    • transient receptor potential-p8 antibody
    • Trp p8 antibody
    • Trp-p8 antibody
    • Trpm8 antibody
    • TRPM8_HUMAN antibody
    • TRPP8 antibody
    see all

图片

  • Anti-TRPM8 antibody (ab3243) at 1/750 dilution + TRPM8 transfected Cos7 cell lysate

    Predicted band size: 128 kDa

  • ab3243  staining TRPM8 in human prostate cancer tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • All lanes : Anti-TRPM8 antibody (ab3243) at 1000 cells

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human hepatocyte lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit HRP at 1/1000 dilution

    Predicted band size: 128 kDa

    See Abreview

  • ab3243 staining TRPM8 in murine skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized with 0.4% Triton X-100, blocked using 10% serum for 30 minutes at 25°C and then incubated with ab3243 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

文献

This product has been referenced in:
  • Liu T  et al. RNA interference-mediated depletion of TRPM8 enhances the efficacy of epirubicin chemotherapy in prostate cancer LNCaP and PC3 cells. Oncol Lett 15:4129-4136 (2018). Read more (PubMed: 29541177) »
  • Klumpp D  et al. TRPM8 is required for survival and radioresistance of glioblastoma cells. Oncotarget 8:95896-95913 (2017). Read more (PubMed: 29221175) »
See all 18 Publications for this product

客户评价及客户问答

1-10 of 15 Q&A

Answer

Gracias por contactarnos, le pido disculpas por la espera.

Adjunto al email la datasheet del producto descatalogado ab3243. Al no estar disponible no tengo acceso a la datasheet en formato pdf, por lo que la envío copiada en un documento Word, siento los inconvenientes.

También le envío el enlace a la búsqueda llevada a cabo de anticuerpos anti TRPM8 humano en WB y IHC (Fr/P).

https://www.abcam.com/index.html?pageconfig=searchresults&search=TRPM8&pt=1&sk=app&sv=74&sn=IHC-P&l=4&fViewMore=1

Si necesitara más información acerca de cualquiera de los productos identificados, un presupuesto o tuviera cualquier otra consulta, por favor, no dude en volvernos a contactar.

Read More

Question
Answer

Thank you for contacting Abcam. Please find the protocol used to test ab3243 in IHC below. If there is anything else I can help you with, please let me know.

Read More

Answer

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab3243 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. I can confirm that our laboratory tested this antibody with transfected Cos cell lysate in western blotting and managed to obtain a band at around 130kDa. Unfortunately, I am unable to retrieve any further protocol information (amount of protein loaded, etc.) and therefore, the image is not suitable for use on our datasheet. I have attached a copy of the image for your reference only. I am unsure of the exact protocols used by Yang et al 2006, but please rest assured that we guarantee our products to perform well in species and applications as stated on the datasheet. If you would like some technical support, I have looked through the protocols you used and have a few questions and suggestions that might help you resolve the problem. In most cases, the cause of multiple bands is because the primary and/or secondary antibody concentration is too high and causes non-specific binding. What was the dilution that you used? The dilution that we have on the datasheet is a recommended starting dilution and customers are encouraged to determine the optimal concentration/dilution. You can try decreasing the primary (1:2000 ~ 1:5000) and secondary antibody concentration or run a no-primary control (without the primary antibody). Running a no-primary control will be able to eliminate the possibility that your secondary antibody is binding non-specifically. Another possible cause of non-specific bands is that the amount of protein is too much. We normally recommend using 20-40 micrograms per well. Please try this amount if you have not already done so. What are the band sizes you obtained? The bands located above the expected molecular weight band could be multimers. Please try boiling your protein in SDS-PAGE for 10 minutes rather than 5 minutes to ensure proper disruption of multimers. Meanwhile, bands located below the expected molecular weight may indicate that your target protein has been digested. Please make sure that you incorporated sufficient protease inhibitors and have kept your samples on ice the whole time. Increasing the washing frequency and duration would also help eliminate non-specific bands. Can you confirm that you have used Tween in your washing solution and have washed at least 15min x 3 times? I hope the above recommendations may already help you, if you still experience problems please do not hesitate to contact me with the answers to the above questions and an image of the blot if possible. I would also appreciate if you could also provide details of your order (purchase order number, shipping address/purchasing agent info, contact information, etc.) so that I can immediately arrange for a replacement or refund to you. Also, please advice on how you would to proceed with this enquiry. I look forward to hearing from you again in order to resolve the matter.

Read More

Answer

Thank you for your e-mail to Hugh who is away today. I'm very sorry to hear you are still experiencing lots of non specific bands with ab3243 even after the protocol modifications. I would be happy to offer you a replacement vial or refund if you purchased the antibody in the last 3 months. Can you please confirm your order details and what you would prefer? I look forward to hearing from you to arrange this,

Read More

Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. We have details of this antibody applied by IHC from customer feedback, but not western blotting. You are obtaining an alarming number of extraneous bands. I would like to recommend that in order to obtain more specific results that you use 3% BSA as a blocking agent. This is routinely employed by our lab team. I would also like to recommend that you increase the percentage of Tween 20 to 0.1% in an effort to address the non-specific nature of this antibody. I would also like to recommend that you perform an overnight block of your membrane using this agent. If this does not improve your results please get back in touch with me and I am happy to provide you with a replacement antibody or credit against your purchase provided that you purchased this antibody within the past 90 days.

Read More

Answer

Thank you for your enquiry. I am sorry but we do not have the immunogen peptide for this antibody in our catalogue. It may have been exhausted. Yes, were you to have the two peptides used to raise this antibody synthesized I can see no reason why they would not serve as peptide inhibition controls for this antibody.

Read More

Answer

Thank you for your patience and for providing some very useful and important details of your Western blot application and your experimental conditions. Looking at our record, I can clearly see that we have 3 complaints about the batch you received and after discussing this problem with my colleagues – certainly I can offer you either a credit note (which you can use with your next purchase) or an immediate refund. Please do get back to me how you would prefer to proceed and I will arrange the appropriate reimbursement for you. I am looking forward to hearing from you soon.

Read More

Answer

Thank you for your email. Ab3243 has been tested for application in Western blot and Immunohistochemistry (Frozen sections); the antibody has not yet been tested for application in ICC. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). Please contact us again if you have any additional questions.

Read More

Question
Answer

Thank you for your phone call and I apologize for the shortage that you received. I'm sending you a free of charge replacement vial; it is on order# 99450 and you should receive it tomorrow. Please let me know if you have any additional questions or concerns.

Read More

Answer

Thank you for your email and I'm sorry to hear that this antibody did not work out for you. I have instructed our accounting department to issue you a credit. If you have any questions regarding this, please contact accounts@abcam.com If I can be of further assistance, please let me know.

Read More

1-10 of 15 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册