概述

  • 产品名称
  • 描述
    兔多克隆抗体to TRPM8
  • 宿主
    Rabbit
  • 特异性
    Reacts with residues 278-292 and the C terminus sequence of the human trpm8 protein.
  • 经测试应用
    适用于: ELISA, IP, IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Guinea pig, Human
  • 免疫原

    Synthetic peptide corresponding to Human TRPM8.
    Sequence:

    RNQLEKYISERTIQD Plus NDLKGLLKEIANKIK

  • 阳性对照
    • WB: Transfected COS cell lysate; human liver tissue lysate; human hepatocyte lysate. PMID 17510704, prostate cells used as positive control. PC-3 suitable negative control.

性能

应用

Our Abpromise guarantee covers the use of ab3243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/100 - 1/2000.
IP Use at an assay dependent concentration.

used at 6 ug/mg cell lysate.

IHC-P 1/10 - 1/2000.
WB 1/500 - 1/1000. Predicted molecular weight: 128 kDa.
IHC-Fr 1/100. See Abreview.

靶标

  • 功能
    Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
  • 组织特异性
    Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
  • 序列相似性
    Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Long transient receptor potential channel 6 antibody
    • LTrpC-6 antibody
    • LTrpC6 antibody
    • MGC2849 antibody
    • Short form of the TRPM8 cationic channel antibody
    • Transient receptor potential cation channel subfamily M member 8 antibody
    • Transient receptor potential p8 antibody
    • transient receptor potential-p8 antibody
    • Trp p8 antibody
    • Trp-p8 antibody
    • Trpm8 antibody
    • TRPM8_HUMAN antibody
    • TRPP8 antibody
    see all

图片

  • Anti-TRPM8 antibody (ab3243) at 1/750 dilution + TRPM8 transfected Cos7 cell lysate

    Predicted band size: 128 kDa

  • ab3243  staining TRPM8 in human prostate cancer tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • All lanes : Anti-TRPM8 antibody (ab3243) at 1000 cells

    Lane 1 : Human liver tissue lysate
    Lane 2 : Human hepatocyte lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit HRP at 1/1000 dilution

    Predicted band size: 128 kDa

    See Abreview

  • ab3243 staining TRPM8 in murine skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized with 0.4% Triton X-100, blocked using 10% serum for 30 minutes at 25°C and then incubated with ab3243 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

文献

This product has been referenced in:
  • Liu T  et al. RNA interference-mediated depletion of TRPM8 enhances the efficacy of epirubicin chemotherapy in prostate cancer LNCaP and PC3 cells. Oncol Lett 15:4129-4136 (2018). Read more (PubMed: 29541177) »
  • Klumpp D  et al. TRPM8 is required for survival and radioresistance of glioblastoma cells. Oncotarget 8:95896-95913 (2017). Read more (PubMed: 29221175) »

See all 17 Publications for this product

客户评价及客户问答

Gracias por contactarnos, le pido disculpas por la espera.

Adjunto al email la datasheet del producto descatalogado ab3243. Al no estar disponible no tengo acceso a la datasheet en formato pdf, por lo que la envío copiada en un documen...

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Thank you for contacting Abcam. Please find the protocol used to test ab3243 in IHC below. If there is anything else I can help you with, please let me know.

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab3243 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the e...

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Thank you for your e-mail to Hugh who is away today. I'm very sorry to hear you are still experiencing lots of non specific bands with ab3243 even after the protocol modifications. I would be happy to offer you a replacement vial or refund if you p...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. We have details of this antibody applied by IHC from customer feedback,...

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Thank you for your enquiry. I am sorry but we do not have the immunogen peptide for this antibody in our catalogue. It may have been exhausted. Yes, were you to have the two peptides used to raise this antibody synthesized I can see no reason why t...

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Thank you for your patience and for providing some very useful and important details of your Western blot application and your experimental conditions. Looking at our record, I can clearly see that we have 3 complaints about the batch you received ...

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Thank you for your email. Ab3243 has been tested for application in Western blot and Immunohistochemistry (Frozen sections); the antibody has not yet been tested for application in ICC. If you decide to go ahead and purchase this product, please let...

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Thank you for your phone call and I apologize for the shortage that you received. I'm sending you a free of charge replacement vial; it is on order# 99450 and you should receive it tomorrow. Please let me know if you have any additional questions or...

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Thank you for your email and I'm sorry to hear that this antibody did not work out for you. I have instructed our accounting department to issue you a credit. If you have any questions regarding this, please contact accounts@abcam.com If I can be o...

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