概述

  • 产品名称
    Anti-TRPM7抗体
    参阅全部 TRPM7 一抗
  • 描述
    山羊多克隆抗体to TRPM7
  • 宿主
    Goat
  • 经测试应用
    适用于: IHC-P, IHC-Fr, ELISA, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Crayfish
    预测可用于: Dog, Pig
  • 免疫原

    Synthetic peptide: TKESESTNSVRLML, corresponding to C terminal amino acids 1852-1865 of Human TRPM7.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Daudi lysate
  • 常规说明

    In response to customer feedback and in-house testing the latest lot doesn't pass the WB quality testing so we have decided to remove the application for now. Please contact technical@abcam.com for more details.

     

    Please see reference He et al for details of the size of the protein detected by SDS-PAGE. Principal Names: TRPM7; transient receptor potential cation channel, subfamily M, member 7; CHAK; CHAK1; LTRPC7; FLJ20117; TRP-PLIK; LTRPC ion channel family member 7; homolog of mouse transient receptor potential-phospholipase C-interacting kinase CHaK hypothetical protein. Official Gene Symbol - TRPM7 GenBank Accession Number – XP_030709 LocusLink ID - 54822 (human).

     Can be blocked with Human TRPM7 peptide (ab22777).

     

性能

应用

Our Abpromise guarantee covers the use of ab729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 21183474
IHC-Fr 1/1000. PubMed: 16285951
ELISA 1/16000.
ICC/IF Use at an assay dependent concentration. PubMed: 16285951
IHC-FoFr Use at an assay dependent concentration. PubMed: 19734892

靶标

  • 功能
    Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
  • 疾病相关
    Defects in TRPM7 are a cause of susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1) [MIM:105500]; also called amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam or Guam disease. Amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 is a neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual.
  • 序列相似性
    In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
    In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
    Contains 1 alpha-type protein kinase domain.
  • 翻译后修饰
    Autophosphorylated.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • ALSPDC antibody
    • CHAK antibody
    • CHAK1 antibody
    • Channel kinase 1 antibody
    • Channel-kinase 1 antibody
    • Long transient receptor potential channel 7 antibody
    • LTrpC-7 antibody
    • LTrpC7 antibody
    • Transient receptor potential cation channel subfamily M member 7 antibody
    • TRP PLIK antibody
    • TRPM7 antibody
    • TRPM7_HUMAN antibody
    see all

文献

This product has been referenced in:
  • Liu A  et al. TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury. Sci Rep 8:5510 (2018). Read more (PubMed: 29615639) »
  • Gao SL  et al. TRPM7 is overexpressed in bladder cancer and promotes proliferation, migration, invasion and tumor growth. Oncol Rep 38:1967-1976 (2017). Read more (PubMed: 28791418) »

See all 16 Publications for this product

发表研究结果有使用 ab729？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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20 reviews or Q&A

10
Votes: Highest First

Here is the figure of the manuscript that shows that specific siRNAs reduce the levels of TRPM7.

Best regards

Could you send us the following information;

- lot number
- purchase order number

Thanks!

For your knowledge, I enclose the comments of two reviewers to a paper that we submitted to an international journal. We addressed the role of TRPM7 in endothelial cells and used your anti-TRPM7 antibody Ab729 e 72155 . Our manuscript was rejected bec...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and would like to investigate the complaint further. Could you send us the publication o...

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Many thanks for your prompt reply. I look forward to hearing of any results that you get .

I am also looking for an antibody against TRPM7 that will react in bovine cells. Ab 72155 looks a possibility since it is given as reacting with human...

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Thank you for your reply.


For anti-TRPM7 the immunogen region for ab729 and ab72155 show 100% homology with the bovine sequence. Therefore, either of these antibodies should work with bovine.

http://www.uniprot.org/uniprot/F1MP...

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We are using the Abcam antibody directed against TRPM7 (ab729). The datasheet mentions that WB gives a band at 160 while the molecular weight of TRPM7 is 220kD. Do you have any idea why this is the case? And can we expect the same for TRPM6 or not nece...

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Thank you for contacting us.

The ab let me know the following:
The observed molecular weight corresponds to earlier findings in the literature with different antibodies. We are not sure if this applies also to TRPM6.

I hope t...

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We are using the Abcam antibody directed against TRPM7 (ab729). The datasheet mentions that WB gives a band at 160 while the molecular weight of TRPM7 is 220kD. Do you have any idea why this is the case? And can we expect the same for TRPM6 or not nece...

Read More

Thank you for contacting us.

I am checking with the lab regarding ab729 and will let you know.

As for TRPM6, which antibody are you refering to?

I look forward to hear back from you.

Use our products? Submit a...

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I want ab85016 and ab729 with a different lot number (GR23197-4 and GR44781-1, respectively).

Thank you for your reply. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have iss...

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Thank you so much for your timely response...
We would love to have replacements shipped to ....

Thank you for your email.

I can definitely send you 2 replacement antibodies to the address you mentioned, but I would need to know which ones you'd like to receive.

I can send the same antibodies and different lots if we have them...

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Our lab's order number is xxx. xxxxx ordered it last year.

Thank you for your reply.

I have located both your orders for ab729 and ab85016.

Could you please let me know if you would prefer a free of charge replacement for each of the antibodies, a credit or refund? We do carry other TRPM7 ...

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Catalog number(s): 85016

Product(s): Mouse monoclonal [S74-25] to TRPM7

Survey results
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Telephone section
Q. Did you contact Abcam's ...

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Thank you for your recent participation in our survey.
I am very sorry to hear that the protocol tips did not work.

You had used ab729 as well as ab85016.What tips have you tried and have the results changed at all?

Please let ...

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Our lab ordered and accepted  monoclonal anti-TRPM7 antibody (#ab85016) and polyclonal antibody (#ab729). However we are not able to get  ~216kDa or ~160 KD band of WB in HUVEC cells and HEK cells. Briefly, we used NP40 buffer to lyse washed cells. Lys...

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Thank you for contacting us. I have received WB protocol information for ab729, but am still waiting for more information regarding ab85016. Thank you for your continued patience in the meantime. For ab729, we have seen a 150-160kDa band in DDI, ...

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1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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