Anti-TRPM7抗体(ab729)
Key features and details
- Goat polyclonal to TRPM7
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human, Crayfish
- Isotype: IgG
概述
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产品名称
Anti-TRPM7抗体
参阅全部 TRPM7 一抗 -
描述
山羊多克隆抗体to TRPM7 -
宿主
Goat -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, Crayfish
预测可用于: Dog, Pig -
免疫原
Synthetic peptide: TKESESTNSVRLML, corresponding to C terminal amino acids 1852-1865 of Human TRPM7.
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常规说明
In response to customer feedback and in-house testing the latest lot doesn't pass the WB quality testing so we have decided to remove the application for now. Please contact technical@abcam.com for more details.
Please see reference He et al for details of the size of the protein detected by SDS-PAGE. Principal Names: TRPM7; transient receptor potential cation channel, subfamily M, member 7; CHAK; CHAK1; LTRPC7; FLJ20117; TRP-PLIK; LTRPC ion channel family member 7; homolog of mouse transient receptor potential-phospholipase C-interacting kinase CHaK hypothetical protein. Official Gene Symbol - TRPM7 GenBank Accession Number – XP_030709 LocusLink ID - 54822 (human).
Can be blocked with Human TRPM7 peptide (ab22777).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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IHC-P | Use at an assay dependent concentration. | |
ICC/IF | Use at an assay dependent concentration. |
靶标
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功能
Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1). -
疾病相关
Defects in TRPM7 are a cause of susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1) [MIM:105500]; also called amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam or Guam disease. Amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 is a neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual. -
序列相似性
In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
Contains 1 alpha-type protein kinase domain. -
翻译后修饰
Autophosphorylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 54822 Human
- Omim: 605692 Human
- SwissProt: Q96QT4 Human
- Unigene: 512894 Human
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别名
- ALSPDC antibody
- CHAK antibody
- CHAK1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence analysis of U2OS labeling TRPM7 with ab729 at 10 ug/mL. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 antibody was used as the secondary antibody (2ug/mL). Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/mL) followed by Alexa Fluor 488 secondary antibody (2ug/mL).
数据表及文件
文献 (21)
ab729 被引用在 21 文献中.
- Lee S et al. The Presence and Distribution of TRPM7 in the Canine Mammary Glands. Animals (Basel) 10:N/A (2020). PubMed: 32168794
- Xing J et al. TRPM7 channel inhibition exacerbates pulmonary arterial hypertension through MEK/ERK pathway. Aging (Albany NY) 11:4050-4065 (2019). PubMed: 31219801
- Won J et al. Molecular expression of Mg2+ regulator TRPM7 and CNNM4 in rat odontoblasts. Arch Oral Biol 96:182-188 (2018). PubMed: 30278312
- Liu A et al. TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury. Sci Rep 8:5510 (2018). PubMed: 29615639
- Gao SL et al. TRPM7 is overexpressed in bladder cancer and promotes proliferation, migration, invasion and tumor growth. Oncol Rep 38:1967-1976 (2017). PubMed: 28791418