Anti-TRPM7抗体(ab729)

Could you send us the following information;

- lot number
- purchase order number

Thanks!

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and would like to investigate the complaint further. Could you send us the publication or the data you have gained with this antibody? Could you also fill the attached questionnaire?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Thank you for your reply.


For anti-TRPM7 the immunogen region for ab729 and ab72155 show 100% homology with the bovine sequence. Therefore, either of these antibodies should work with bovine.

http://www.uniprot.org/uniprot/F1MPF0

If you'd like to test a TRPM7 antibody in bovine, let me know and I can enroll you in our testing discount program we talked about on the phone.

Thank you for contacting us.

The ab let me know the following:
The observed molecular weight corresponds to earlier findings in the literature with different antibodies. We are not sure if this applies also to TRPM6.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.

I am checking with the lab regarding ab729 and will let you know.

As for TRPM6, which antibody are you refering to?

I look forward to hear back from you.

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Thank you for your reply. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order numbers xxx for ab729 and xxx for ab85016, respectively.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that these free of charge replacement vials are also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your email.

I can definitely send you 2 replacement antibodies to the address you mentioned, but I would need to know which ones you'd like to receive.

I can send the same antibodies and different lots if we have them. I can send other TRPM7 antibodies than the ones you already tried (e.g. ab72155, ab63088). Alternatively, if you need any other antibody to a different target than TRPM7,I can send those too, but would need to know which target protein and antibody you might be looking for.

No, the antibodies you had tried do not need to be returned to us.

I look forward to hear back from you with more information.

Thank you for your reply.

I have located both your orders for ab729 and ab85016.

Could you please let me know if you would prefer a free of charge replacement for each of the antibodies, a credit or refund? We do carry other TRPM7 antibodies. Alternatively, you can also choose antibodies to a different target protein.

I look forward to hear back and resolve this case for you.

Thank you for your recent participation in our survey.
I am very sorry to hear that the protocol tips did not work.

You had used ab729 as well as ab85016.What tips have you tried and have the results changed at all?

Please let me know your order or PO number for these antibodies or the institution or order date so that I can locate your order in our system.

If the products were purchased within last year, I'd be happy to offer you either a free of charge repalcement, a credit or a refund.

I look forward to hear back from you and resolve this for you.

Thank you for contacting us. I have received WB protocol information for ab729, but am still waiting for more information regarding ab85016. Thank you for your continued patience in the meantime. For ab729, we have seen a 150-160kDa band in DDI, and in human brain lysates. All samples are boiled in Laemmli sample buffer before loading. The standard WB conditions are as follows: Starting material: We find enhancement of transfer of proteins on PVDF membranes compared to nitrocellulose membranes. • The membrane is blocked in 3% (w/v) skimmed milk in TBS-T for 1 hr at room temperature with agitation (can be blocked overnight at 4°C without agitation). • Primary antibody is incubated in the blocking buffer for 1 hr at room temperature with agitation. • The anti-goat secondary antibody should be used according to manufacturer's recommendations. Ideally, with minimal cross-reactivity with human and rodent serum proteins. It should be diluted in the blocking buffer and incubated for 1 hr at room temperature with agitation. • Wash with TBST three times after primary and after secondary antibody, each wash lasting for 5-10 minutes. • Use ECL-plus rather than ECL, which is more sensitive for detection. The blots are exposed to X-ray film. Tris buffered saline (TBS): 20mM Tris, pH7.4 in 150mM NaCl TBS-T: TBS with 0.05% v/v Tween-20 Important comments: After transfer on PVDF membrane, the quality of the transfer is monitored by staining in Ponceau red. Areas with visible air bubbles are marked with permanent ink and lanes where an air bubble was trapped at the position of bands of interest should be avoided for analysis. The Ponceau is removed by Tween-containing buffer. Primary incubation overnight at 4C requires much further dilution of the primary antibody. Still, non-specific background may be expected when incubating overnight.   I might suggest trying to use RIPA buffer as lysis buffer for your samples, as the buffer is stronger than NP40 and will be able to keep the hydrophobic protein in solution better. Also, adding up to 0.1% SDS to the transfer buffer can help prevent that the protein precipitates in the gel during the transfer. Please also let me know the answers to my questions in my last email as this will be of help to determine the cause of the problem better. I hope this information is helpful to you. I will be in touch regarding ab85016. Please let me know if any of the suggestions are of help. And if not, please let me know your order or PO number so that we can resolve this issue to your satisfaction.