Anti-Trophoblast specific蛋白alpha抗体(ab104401)
Key features and details
- Rabbit polyclonal to Trophoblast specific protein alpha
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Trophoblast specific蛋白alpha抗体 -
描述
兔多克隆抗体to Trophoblast specific蛋白alpha -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Mouse Trophoblast specific protein alpha aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab117405) -
阳性对照
- This antibody gave a positive signal in Mouse trophoblast tissue lysate as well as the following whole cell lysates: MEF1; NIH3T3; STO.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104401于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. |
靶标
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相关性
Trophoblast specific protein alpha is a marker of spongiotrophoblasts. -
数据库链接
- Entrez Gene: 21984 Mouse
- SwissProt: P13438 Mouse
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别名
- Tb1 antibody
- Tpbp antibody
- Tpbpa antibody
see all
图片
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All lanes : Anti-Trophoblast specific protein alpha antibody (ab104401) at 1 µg/ml
Lane 1 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Trophoblast Stem Cells (Mouse) Whole Cell Lysate
Lane 4 : STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Additional bands at: 150 kDa, 255 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 14 minutes -
ICC/IF image of ab104401 stained MEF1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96899, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab104401, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) MEF1 cells at 1µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab104401 被引用在 25 文献中.
- Hufnagel A et al. Maternal but not fetoplacental health can be improved by metformin in a murine diet-induced model of maternal obesity and glucose intolerance. J Physiol 600:903-919 (2022). PubMed: 34505282
- Chen Z et al. Maternal exposure to CeO2NPs derails placental development through trophoblast dysfunction mediated by excessive autophagy activation. J Nanobiotechnology 20:131 (2022). PubMed: 35292031
- Zhang W et al. Rif1 and Hmgn3 regulate the conversion of murine trophoblast stem cells. Cell Rep 38:110570 (2022). PubMed: 35354046
- Rusidzé M et al. Loss of function of the maternal membrane oestrogen receptor ERα alters expansion of trophoblast cells and impacts mouse fertility. Development 149:N/A (2022). PubMed: 36239412
- Kinisu M et al. Klf5 establishes bi-potential cell fate by dual regulation of ICM and TE specification genes. Cell Rep 37:109982 (2021). PubMed: 34758315