Anti-Tristetraprolin/TTP抗体[OTI8B5] (ab124024)
Key features and details
- Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-Tristetraprolin/TTP抗体[OTI8B5]
参阅全部 Tristetraprolin/TTP 一抗 -
描述
小鼠单克隆抗体[OTI8B5] to Tristetraprolin/TTP -
宿主
Mouse -
经测试应用
适用于: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human Tristetraprolin/TTP.
Database link: NP_003398 -
阳性对照
- HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA; Human colon, colon adenocarcinoma, ovary, endometrium and prostate tissues; COS7 cells transiently transfected by pCMV6-ENTRY Tristetraprolin/TTP.
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常规说明
Dilute in PBS (pH7.3) before use.
The clone number has been updated from 8B5 to OTI8B5, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
OTI8B5 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124024于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50.
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1 - 1/1000.
|
说明 |
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IHC-P
1/50. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/1 - 1/1000. |
靶标
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功能
mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). -
序列相似性
Contains 2 C3H1-type zinc fingers. -
翻译后修饰
Phosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation. -
细胞定位
Nucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules. - Information by UniProt
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数据库链接
- Entrez Gene: 7538 Human
- Omim: 190700 Human
- SwissProt: P26651 Human
- Unigene: 534052 Human
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别名
- G0/G1 switch regulatory protein 24 antibody
- G0S24 antibody
- GOS24 antibody
see all
图片
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All lanes : Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA
Lysates/proteins at 5 µg per lane.
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202049) -
ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human colon tissue by Immunohistochemistry.
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ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
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ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human ovary tissue by Immunohistochemistry.
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ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human endometrium tissue by Immunohistochemistry.
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ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human prostate tissue by Immunohistochemistry.
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ab124024, at 1/100, staining Tristetraprolin/TTP in COS7 cells transiently transfected with pCMV6-ENTRY Tristetraprolin/TTP by Immunofluorescence.
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ab124024 at 1/100 dilution staining Tristetraprolin/TTP in HEK293T cells transfected with either pCMV6-ENTRY Tristetraprolin/TTP (Red) or empty vector control plasmid (Blue) by Flow Cytometry (Intracellular).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab124024 被引用在 4 文献中.
- Fakhfakh R et al. Differential Expression of Anti-Inflammatory RNA Binding Proteins in Lupus Nephritis. Life (Basel) 12:N/A (2022). PubMed: 36294909
- Wang M et al. Nicotine-mediated OTUD3 downregulation inhibits VEGF-C mRNA decay to promote lymphatic metastasis of human esophageal cancer. Nat Commun 12:7006 (2021). PubMed: 34853315
- Na L et al. Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves Spinal Cord Injury via MK2/TTP Signalling Pathway. Biomed Res Int 2020:3021750 (2020). PubMed: 33376718
- Selmi T et al. ZFP36 stabilizes RIP1 via degradation of XIAP and cIAP2 thereby promoting ripoptosome assembly. BMC Cancer 15:357 (2015). PubMed: 25939870