• 产品名称
    参阅全部 Tristetraprolin 一抗
  • 描述
    兔多克隆抗体to Tristetraprolin
  • 宿主
  • 特异性
    Does not react with Mouse, based on IHC-P Abreview.
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Sheep, Rabbit, Guinea pig, Cow, Cat, Dog不与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Tristetraprolin aa 84-133.


    Database link: P26651
    (Peptide available as ab111664)

  • 阳性对照
    • HepG2 cell lysate.



Our Abpromise guarantee covers the use of ab33058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Can be blocked with Human Tristetraprolin peptide (ab111664). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 µg/ml.


  • 功能
    mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF).
  • 序列相似性
    Contains 2 C3H1-type zinc fingers.
  • 翻译后修饰
    Phosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation.
  • 细胞定位
    Nucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G0/G1 switch regulatory protein 24 antibody
    • G0S24 antibody
    • GOS24 antibody
    • Growth factor-inducible nuclear protein NUP475 antibody
    • NUP475 antibody
    • Protein TIS11A antibody
    • RNF162A antibody
    • TIS 11 antibody
    • TIS11 antibody
    • TIS11A antibody
    • Tristetraprolin antibody
    • Tristetraproline antibody
    • TTP antibody
    • TTP_HUMAN antibody
    • Zfp-36 antibody
    • ZFP36 antibody
    • Zinc finger protein 36 antibody
    • Zinc finger protein 36 homolog antibody
    • Zinc finger protein 36, C3H type, homolog (mouse) antibody
    • Zinc finger protein, C3H type, 36 homolog antibody
    see all


  • Lane 1 : Anti-Tristetraprolin antibody (ab33058) at 2 µg/ml
    Lane 2 : Anti-Tristetraprolin antibody (ab33058) at 1 µg/ml
    Lane 3 : Anti-Tristetraprolin antibody (ab33058) at 0.5 µg/ml

    All lanes : Cell lysate prepared from human HepG2 cells

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa
    Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.

  • ab33058 (4µg/ml) staining Tristetraprolin in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic and nuclei of macrophages and nuclei staining in some pneumocytes. There was also cytoplasmic and nuclei staining of epithelium cells of the bronchioles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal ampli
  • ICC/IF image of ab33058 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33058, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Wang H  et al. Dysregulation of tristetraprolin and human antigen R promotes gastric cancer progressions partly by upregulation of the high-mobility group box 1. Sci Rep 8:7080 (2018). Read more (PubMed: 29728635) »
  • Wu X  et al. The ARE-binding protein Tristetraprolin (TTP) is a novel target and mediator of calcineurin tumor suppressing function in the skin. PLoS Genet 14:e1007366 (2018). Read more (PubMed: 29723192) »

See all 9 Publications for this product


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I am sorry to hear that this product is not performing as expected. This product is covered by our Abpromise guarantee. If this is used according to the specification listed on the datasheet (on human in IHC-P,...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab124024.

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Thank you for contacting Abcam regarding ab14371 and ab33058. I have checked the datasheets for both of these antibodies, and they have not yet been tested in EMSA. Therefore, we do not know if these products will work in this assay and cannot guar...

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Currently we have two anti-TTP antibodies, however, neither of them are specific to the phosphorylated form of the protein. I am sorry that I could not be of more help. Please feel free to contact us again should you have further questions about the pr...

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