Application
Western blot
Sample
Human Tissue lysate - whole (Skeletal Muscle)
Loading amount
20 µg
Specification
Skeletal Muscle
Gel Running Conditions
Non-reduced Denaturing (10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: TTBS 1% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase labelled anti-rabbit antibody (Thermo S
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
FEMTO (Fisher)
Exposure
10 second(s)
Bands
Specific: 27 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Maria Insenser
Verified customer
提交于 Jan 27 2012