Anti-TRIM4抗体(ab4527)
Key features and details
- Goat polyclonal to TRIM4
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRIM4抗体
参阅全部 TRIM4 一抗 -
描述
山羊多克隆抗体to TRIM4 -
宿主
Goat -
特异性
This antibody is expected to recognise both human isoforms. -
经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human TRIM4 aa 489-500 (C terminal).
Sequence:C-ASLVIPPVTDRK
(Peptide available asab23042) -
常规说明
GenBank Accession Number – NP_148977; NP_149082.Previously labelled as RNF87.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab4527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | ||
| WB |
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55-58 kDa (predicted molecular weight: 55-60 kDa according to NP_148977 and NP_149082).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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序列相似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. - Information by UniProt
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数据库链接
- Entrez Gene: 89122 Human
- SwissProt: Q9C037 Human
- Unigene: 50749 Human
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别名
- RING finger protein 87 antibody
- RNF 87 antibody
- RNF87 antibody
see all
图片
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Western blot - Anti-TRIM4 antibody (ab4527)ab4527 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
ab4527 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
数据表及文件
文献 (1)
ab4527 被引用在 1 文献中.
- Feyeux M et al. Early transcriptional changes linked to naturally occurring Huntington's disease mutations in neural derivatives of human embryonic stem cells. Hum Mol Genet 21:3883-95 (2012). PubMed: 22678061
