Anti-TRIM4抗体(ab4527)

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Western blot - Anti-TRIM4 antibody (ab4527)

    Key features and details

    • Goat polyclonal to TRIM4
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-TRIM4抗体
      参阅全部 TRIM4 一抗

    • 描述

      山羊多克隆抗体to TRIM4

    • 宿主

      Goat

    • 特异性

      This antibody is expected to recognise both human isoforms.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide corresponding to Human TRIM4 aa 489-500 (C terminal).
      Sequence:

      C-ASLVIPPVTDRK


      (Peptide available as ab23042)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      GenBank Accession Number – NP_148977; NP_149082.

      Previously labelled as RNF87. 

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4527于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    说明
  • 应用说明
    Peptide ELISA: 1/32000.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55-58 kDa (predicted molecular weight: 55-60 kDa according to NP_148977 and NP_149082).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 序列相似性

      Belongs to the TRIM/RBCC family.
      Contains 1 B box-type zinc finger.
      Contains 1 B30.2/SPRY domain.
      Contains 1 RING-type zinc finger.
    • Information by UniProt

    • 数据库链接

      • 别名

        • RING finger protein 87 antibody
        • RNF 87 antibody
        • RNF87 antibody
        • TRIM4 antibody
        • TRIM4_HUMAN antibody
        • Tripartite motif containing 4 antibody
        • Tripartite motif protein 4 antibody
        • Tripartite motif protein TRIM4 antibody
        • Tripartite motif-containing protein 4 antibody
        see all

      图片

      • Western blot - Anti-TRIM4 antibody (ab4527)
        Western blot - Anti-TRIM4 antibody (ab4527)

        ab4527 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

         

        ab4527 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

      文献 (1)

      发表研究结果有使用 ab4527？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab4527 被引用在 1 文献中.

      • Feyeux M  et al. Early transcriptional changes linked to naturally occurring Huntington's disease mutations in neural derivatives of human embryonic stem cells. Hum Mol Genet 21:3883-95 (2012). PubMed: 22678061

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