概述

  • 产品名称
  • 描述
    兔多克隆抗体to TRIM36
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Common marmoset
    预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Rat TRIM36 aa 166-215 (N terminal).
    Sequence:

    ESTKSCMDCSASYCNECFKIYHPWGTVKAQHEYVGPTTNFRPKILMCPEH


    Database link: NP_001099617.1

  • 阳性对照
    • Rat lung lysate.
  • 常规说明

    Previously labelled as RNF98.

性能

应用

Our Abpromise guarantee covers the use of ab116212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization.
  • 组织特异性
    Highly expressed in testis, prostate and brain. Weakly expressed in kidney, lung and heart.
  • 序列相似性
    Belongs to the TRIM/RBCC family.
    Contains 2 B box-type zinc fingers.
    Contains 1 B30.2/SPRY domain.
    Contains 1 COS domain.
    Contains 1 fibronectin type-III domain.
    Contains 1 RING-type zinc finger.
  • 细胞定位
    Cytoplasm. Cytoplasmic vesicle > secretory vesicle > acrosome. Cytoplasm > cytoskeleton. Found in the acrosomal region of elongated spermatids and mature sperm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acrosome RBCC protein antibody
    • E3 ubiquitin protein ligase TRIM36 antibody
    • E3 ubiquitin-protein ligase Trim36 antibody
    • Haprin antibody
    • RBCC728 antibody
    • RING finger protein 98 antibody
    • RNF98 antibody
    • TRI36_HUMAN antibody
    • TRIM 36 antibody
    • Trim36 antibody
    • Tripartite motif containing 36 antibody
    • Tripartite motif containing protein 36 antibody
    • Tripartite motif protein 36 antibody
    • Tripartite motif-containing protein 36 antibody
    • Zinc binding protein Rbcc728 antibody
    • Zinc-binding protein Rbcc728 antibody
    see all

图片

  • IHC-P image of TRIM36 staining with ab116212 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab116212 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated Goat anti-Rabbit polyclonal, used at a 1/500 dilution.

    See Abreview

  • Anti-TRIM36 antibody (ab116212) at 1 µg/ml + Rat lung lysate at 10 µg

    Predicted band size: 83 kDa



    Gel concentration 12%

文献

ab116212 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Adult Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Specification
Adult Testis
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Dr. 煜欽 林

Verified customer

提交于 Oct 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册