存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab116212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
功能E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation. May play a role in the acrosome reaction and fertilization.
组织特异性Highly expressed in testis, prostate and brain. Weakly expressed in kidney, lung and heart.
序列相似性Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 B30.2/SPRY domain.
Contains 1 COS domain.
Contains 1 fibronectin type-III domain.
Contains 1 RING-type zinc finger.
细胞定位Cytoplasm. Cytoplasmic vesicle > secretory vesicle > acrosome. Cytoplasm > cytoskeleton. Found in the acrosomal region of elongated spermatids and mature sperm.
- Information by UniProt
- Acrosome RBCC protein antibody
- E3 ubiquitin protein ligase TRIM36 antibody
- E3 ubiquitin-protein ligase Trim36 antibody
IHC-P image of TRIM36 staining with ab116212 on tissue sections from adult marmoset testis. The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab116212 (1/100 dilution) for 18 hours at 4°C. The secondary was an Alexa-Fluor 555 conjugated Goat anti-Rabbit polyclonal, used at a 1/500 dilution.
Anti-TRIM36 antibody (ab116212) at 1 µg/ml + Rat lung lysate at 10 µg
Predicted band size: 83 kDa
Gel concentration 12%
ab116212 has not yet been referenced specifically in any publications.