Key features and details
- Goat polyclonal to TRIM21/SS-A
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
参阅全部 TRIM21/SS-A 一抗
经测试应用适用于: IHC-P, ICC/IF, Flow Cyt, WBmore details
- WB: HEK293 lysate overexpressing human TRIM21/SS-A. IHC-P: Human colon tissue. ICC/IF: U2OS cells. Flow Cyt: HeLa cells.
Previously labelled as TRIM21
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab4369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 0.2 - 1 µg/ml.
A 1 hour primary incubation is recommended for this product. In transfected HEK293 transiently expressing full-length Human TRIM21/SS-A (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody
功能E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2.
组织特异性Isoforms 1 and 2 are expressed in fetal and adult heart and fetal lung.
通路Protein modification; protein ubiquitination.
序列相似性Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
结构域The coiled-coil is necessary for the cytoplasmic localization. The B30.2/SPRY domain is necessary for the cytoplasmic localization, the interaction with IRF3 and for the IRF3-driven interferon beta promoter activity. The RING-type zinc finger is necessary for ubiquitination and for the IRF3-driven interferon beta promoter activity. Interacts with SKP2 and CUL1 in a RING finger-independent manner.
翻译后修饰Autoubiquitinated; does not lead to its proteasomal degradation. Deubiquitinated by USP4; leading to its stabilization.
细胞定位Cytoplasm. Nucleus. Cytoplasm > P-body. Enters the nucleus upon exposure to nitric oxide. Localizes to small dot- or rod-like structures in the cytoplasm, called cytoplasmic bodies (P-body) that are located underneath the plasma membrane and also diffusely in the cytoplasm and are highly motil in cells. Cytoplasmic bodies are located along the microtubules and do not share the same cytoplasmic bodies with TRIM5. Colocalizes with DCP2 in P-body.
- Information by UniProt
- 52 kDa ribonucleoprotein autoantigen Ro/SS-A antibody
- 52 kDa Ro protein antibody
- 52kD Ro/SSA autoantigen antibody
Lanes 1-2 : Anti-TRIM21/SS-A antibody (ab4369) at 1 µg/ml
Lane 3 : Anti-Myc tag antibody at 1/1000 dilution
Lanes 1 & 3 : HEK293 lysate overexpressing Myc-tagged SSA1 (in RIPA buffer)
Lane 2 : Control HEK293 lysate (in RIPA buffer)
Lysates/proteins at 10 µg per lane.
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Primary incubation for 1 hour. Detected using chemiluminescence.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling TRIM21/SS-A with ab4369 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
Flow cytometry analysis of HeLa cells labelling TRIM21/SS-A with ab4369 (blue line) at 10 µg/ml. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton. Incubated with the primary antibody for 1 hour followed by incubation with Alexa Fluor® 488-conjugated secondary antibody at 1 µg/ml.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488-conjugated secondary antibody.
ab4369 被引用在 1 文献中.
- Kuboshima M et al. Presence of serum tripartite motif-containing 21 antibodies in patients with esophageal squamous cell carcinoma. Cancer Sci 97:380-6 (2006). PubMed: 16630135