Key features and details
- Goat polyclonal to TRIM2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
经测试应用适用于: IHC-P, WBmore details
种属反应性与反应: Mouse, Rat, Human
预测可用于: Cow, Dog
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab3942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 µg/ml.
A 1 hour primary incubation is recommended for this product.
功能May contribute to the alteration of neural cellular mechanisms.
序列相似性Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 filamin repeat.
Contains 6 NHL repeats.
Contains 1 RING-type zinc finger.
结构域The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
- Information by UniProt
- E3 ubiquitin protein ligase TRIM2 antibody
- KIAA0517 antibody
- RING finger protein 86 antibody
Anti-TRIM2 antibody (ab3942) at 0.5 µg/ml + Rat brain tissue lysate at 35 µg
Observed band size: 80,85 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was for 1 hour. Detected by chemiluminescence.
ab3942 at 6.3 µg/ml staining TRIM2 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.