Anti-TRIM2抗体(ab3942)
Key features and details
- Goat polyclonal to TRIM2
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-TRIM2抗体 -
描述
山羊多克隆抗体to TRIM2 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Dog
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免疫原
Synthetic peptide corresponding to Human TRIM2 aa 731-744 (C terminal).
Sequence:DSGNHCFKVYRYLQ
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.5 µg/ml. A 1 hour primary incubation is recommended for this product. |
靶标
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功能
May contribute to the alteration of neural cellular mechanisms. -
序列相似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 filamin repeat.
Contains 6 NHL repeats.
Contains 1 RING-type zinc finger. -
结构域
The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 23321 Human
- Entrez Gene: 80890 Mouse
- Entrez Gene: 361970 Rat
- Omim: 614141 Human
- SwissProt: Q9C040 Human
- SwissProt: Q9ESN6 Mouse
- SwissProt: D3ZQG6 Rat
- Unigene: 435711 Human
see all -
别名
- E3 ubiquitin protein ligase TRIM2 antibody
- KIAA0517 antibody
- RING finger protein 86 antibody
see all
图片
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Western blot - Anti-TRIM2 antibody (ab3942)Anti-TRIM2 antibody (ab3942) at 0.5 µg/ml + Rat brain tissue lysate at 35 µg
Observed band size: 80,85 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.Primary incubation was for 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM2 antibody (ab3942)ab3942 at 6.3 µg/ml staining TRIM2 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
数据表及文件
文献 (0)
ab3942 尚未被引用在任何文献中。
