Anti-TRIM2抗体(ab3942)
Key features and details
- Goat polyclonal to TRIM2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-TRIM2抗体 -
描述
山羊多克隆抗体to TRIM2 -
宿主
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat
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免疫原
Synthetic peptide corresponding to Human TRIM2 aa 731-744 (C terminal).
Sequence:DSGNHCFKVYRYLQ
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Rat
|
| All applications |
Cow
Dog
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.5 µg/ml.
A 1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.5 µg/ml. A 1 hour primary incubation is recommended for this product. |
靶标
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功能
May contribute to the alteration of neural cellular mechanisms. -
序列相似性
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 filamin repeat.
Contains 6 NHL repeats.
Contains 1 RING-type zinc finger. -
结构域
The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 23321 Human
- Entrez Gene: 361970 Rat
- Omim: 614141 Human
- SwissProt: Q9C040 Human
- SwissProt: D3ZQG6 Rat
- Unigene: 435711 Human
- Unigene: 16973 Rat
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别名
- E3 ubiquitin protein ligase TRIM2 antibody
- KIAA0517 antibody
- RING finger protein 86 antibody
see all
图片
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Western blot - Anti-TRIM2 antibody (ab3942)Anti-TRIM2 antibody (ab3942) at 0.5 µg/ml + Rat brain tissue lysate at 35 µg
Observed band size: 80,85 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.Primary incubation was for 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM2 antibody (ab3942)ab3942 at 6.3 µg/ml staining TRIM2 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
实验方案
数据表及文件
文献 (0)
ab3942 尚未被引用在任何文献中。
