Anti-TRIM2抗体(ab3942)

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Key features and details

  • Goat polyclonal to TRIM2
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-TRIM2抗体

  • 描述

    山羊多克隆抗体to TRIM2

  • 宿主

    Goat

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog

  • 免疫原

    Synthetic peptide corresponding to Human TRIM2 aa 731-744 (C terminal).
    Sequence:

    DSGNHCFKVYRYLQ

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab3942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 6 - 12 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml.

A 1 hour primary incubation is recommended for this product.

靶标

  • 功能

    May contribute to the alteration of neural cellular mechanisms.

  • 序列相似性

    Belongs to the TRIM/RBCC family.
    Contains 1 B box-type zinc finger.
    Contains 1 filamin repeat.
    Contains 6 NHL repeats.
    Contains 1 RING-type zinc finger.

  • 结构域

    The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • E3 ubiquitin protein ligase TRIM2 antibody
    • KIAA0517 antibody
    • RING finger protein 86 antibody
    • RNF86 antibody
    • Trim2 antibody
    • TRIM2_HUMAN antibody
    • Tripartite motif containing 2 antibody
    • Tripartite motif protein 2 antibody
    • Tripartite motif protein TRIM2 antibody
    • Tripartite motif-containing 2 antibody
    • Tripartite motif-containing protein 2 antibody
    see all

图片

  • Western blot - Anti-TRIM2 antibody (ab3942)
    Western blot - Anti-TRIM2 antibody (ab3942)
    Anti-TRIM2 antibody (ab3942) at 0.5 µg/ml + Rat brain tissue lysate at 35 µg

    Observed band size: 80,85 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.



    Primary incubation was for 1 hour. Detected by chemiluminescence.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM2 antibody (ab3942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM2 antibody (ab3942)

    ab3942 at 6.3 µg/ml staining TRIM2 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

文献 (0)

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