Anti-TRIF抗体(ab13810)
Key features and details
- Rabbit polyclonal to TRIF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TRIF抗体
参阅全部 TRIF 一抗 -
描述
兔多克隆抗体to TRIF -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Synthetic peptide within Mouse TRIF aa 100-200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q80UF7 -
阳性对照
- WB: Mouse spleen, intestine and RAW 264.7 cell lysates. IHC-P: Mouse spleen tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13810于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 79.23 kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
IHC-P |
1/200.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 79.23 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
IHC-P
1/200. |
靶标
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功能
Involved in innate immunity against invading pathogens. Adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. -
组织特异性
Ubiquitously expressed but with higher levels in liver. -
序列相似性
Contains 1 TIR domain. -
结构域
The N-terminal region is essential for activation of the IFNB promoter activity. -
翻译后修饰
Phosphorylated by TBK1. - Information by UniProt
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数据库链接
- Entrez Gene: 106759 Mouse
- Entrez Gene: 363328 Rat
- SwissProt: Q80UF7 Mouse
- Unigene: 203952 Mouse
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别名
- IIAE6 antibody
- MGC35334 antibody
- MyD88 3 antibody
see all
图片
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All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution
Lane 1 : Mouse spleen lysate with immunizing peptide
Lane 2 : Mouse spleen lysate
Predicted band size: 79.23 kDa -
Immunohistochemistrical analysis of a FFPE tissue section of mouse spleen using 1/200 dilution of ab13810. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
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All lanes : Anti-TRIF antibody (ab13810) at 1/1000 dilution
Lane 1 : Mouse intestine lysate
Lane 2 : Mouse spleen lysate
Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate
Predicted band size: 79.23 kDa
数据表及文件
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Datasheet download
文献 (46)
ab13810 被引用在 46 文献中.
- Kim YK et al. Differential susceptibility to lipopolysaccharide affects the activation of toll-like-receptor 4 signaling in THP-1 cells and PMA-differentiated THP-1 cells. Innate Immun 28:122-129 (2022). PubMed: 35612375
- Muendlein HI et al. ZBP1 promotes inflammatory responses downstream of TLR3/TLR4 via timely delivery of RIPK1 to TRIF. Proc Natl Acad Sci U S A 119:e2113872119 (2022). PubMed: 35666872
- Hornigold K et al. Age-related decline in the resistance of mice to bacterial infection and in LPS/TLR4 pathway-dependent neutrophil responses. Front Immunol 13:888415 (2022). PubMed: 36090969
- Liu Y et al. Antiviral activity of portulaca oleracea L. extracts against porcine epidemic diarrhea virus by partial suppression on myd88/NF-?b activation in vitro. Microb Pathog 154:104832 (2021). PubMed: 33781871
- Nihon-Yanagi Y et al. Immunohistochemical Analysis of Toll-Like Receptors, MyD88, and TRIF in Human Papillary Thyroid Carcinoma and Anaplastic Thyroid Carcinoma. J Thyroid Res 2021:4226491 (2021). PubMed: 34306609