Key features and details
- Rabbit polyclonal to Trichohyalin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
参阅全部 Trichohyalin 一抗
经测试应用适用于: WBmore details
预测可用于: Macaque monkey, Gorilla
Synthetic peptide corresponding to Human Trichohyalin aa 1800-1900 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Skin tissue lysate.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab129814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 210 kDa (predicted molecular weight: 253 kDa).|
功能Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.
组织特异性Found in the hard keratinizing tissues such as the inner root sheath (IRS) of hair follicles and medulla, and in the filiform papillae of dorsal tongue epithelium.
序列相似性Belongs to the S100-fused protein family.
Contains 2 EF-hand domains.
发展阶段Expressed during late differentiation of the epidermis.
结构域Consists of nine domains. Domain 1 contains two EF-hand calcium-binding domains. Domains 2-4, 6, and 8 are almost entirely alpha-helical, configured as a series of peptide repeats of varying regularity, and are thought to form a single-stranded alpha-helical rod stabilized by ionic interactions. Domain 6 is the most regular and may bind KIF directly by ionic interactions. Domains 5 and 7 are less well organized and may induce folds in the molecule. Domain 9 contains the C-terminus, conserved among different species.
翻译后修饰Substrate of transglutaminase. Some 200 arginines are probably converted to citrullines by peptidylarginine deimidase.
- Information by UniProt
- 220K trichohyalin granular protein antibody
- Hair follicle protein AHF antibody
- MGC157889 antibody
Anti-Trichohyalin antibody (ab129814) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 253 kDa
Observed band size: 210 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa, 48 kDa, 55 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
The predicted molecular weight of Trichohyalin is 253 kDa (SwissProt), however we expect to observe a banding pattern around 220 kDa. This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129814 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.