Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (primary dermal fibroblasts from mouse)
Specification
primary dermal fibroblasts from mouse
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% (v/v) Triton X100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Other product details
Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBS + 0.1% TritonX100
Secondary antibody
Name
Non-Abcam antibody was used: goat anti mouse
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Dilution
1/50
Additional data
PubMed ID
19398782: View
Additional Notes
Staining of TG2 in primary mouse dermal fibroblasts (MDF) showing TG2 mainly localised at the cell periphery (green), in accordance with cell-surface TG avtivity measured in primary fibroblasts. Nuclear staining (DAPI) is shown in blue. No signal was detected by performing ICC with anti-mouse secondary antibody only. Bar 20 micrometers
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提交于 Feb 24 2010