Anti-Transglutaminase 2抗体[CUB 7402] (ab2386)
![Western blot - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.cn/ps/products/2/ab2386/Images/ab2386-367611-anti-transglutaminase-2-antibody-cub-7402-western-blot-knockout-hela-huvec-og-a549-wt-human.jpg "Western blot - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)")
Key features and details
- Mouse monoclonal [CUB 7402] to Transglutaminase 2
- Suitable for: IHC-P, WB, Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Transglutaminase 2抗体[CUB 7402]
参阅全部 Transglutaminase 2 一抗 -
描述
小鼠单克隆抗体[CUB 7402] to Transglutaminase 2 -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Transglutaminase 2. Purified from liver.
Database link: P08587 -
表位
Epitope mapping for this antibody is amino acid 447-478 -
阳性对照
- IHC-P: Human breast carcinoma tissue. WB: A549 whole cell lysate; HeLa whole cell lysate; Huvec whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
CUB 7402 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (3) |
1/50 - 1/75. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
|
| Flow Cyt | (1) |
1/10.
Methanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
IHC-P
1/50 - 1/75. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
|
Flow Cyt
1/10. Methanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. -
序列相似性
Belongs to the transglutaminase superfamily. Transglutaminase family. - Information by UniProt
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数据库链接
- Entrez Gene: 7052 Human
- Omim: 190196 Human
- SwissProt: P21980 Human
- Unigene: 517033 Human
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别名
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 2.3.2.13 antibody
see all
图片
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Western blot - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)All lanes : Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386) at 1 µg/ml
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : TGM2 knockout A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Huvec whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1 - 4: Merged signal (red and green). Green - ab2386 observed at 77 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab2386 was shown to recognize in wild-type A549 cells as signal was lost at the expected MW in TGM2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab2386 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.cn/ps/products/2/ab2386/Images/ab2386-391638-anti-transglutaminase-2-antibody-cub-7402-immunohistochemistry-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)Immunohistochemical analysis of breast carcinoma tissue labeling Transglutaminase 2 with ab2386. Tissue was fixed with paraformaldehyde; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 30 minutes at 22°C.
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![Flow Cytometry - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.cn/ps/products/2/ab2386/Images/ab2386-5751-anti-transglutaminase-2-antibody-cub-7402-flow-cytometry.jpg)
Flow Cytometry - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)Overlay histogram showing HCT116 cells stained with ab2386 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2386, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)](https://www.abcam.cn/ps/products/2/ab2386/Images/ab2386-275686-anti-transglutaminase-2-antibody-cub-7402-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [CUB 7402] (ab2386)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) rat lung tissue labeling Transglutaminase 2 with ab2386. Tissue was fixed with formaldehyde and blocked with 1% casein in PBS for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/200 in TBS + BSA) for 20 minutes. ab98782 was used as the secondary antibody at a dilution of 1/400.
实验方案
数据表及文件
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Datasheet download
文献 (23)
ab2386 被引用在 23 文献中.
- Potilinski MC et al. Elucidating the mechanism of action of alpha-1-antitrypsin using retinal pigment epithelium cells exposed to high glucose. Potential use in diabetic retinopathy. PLoS One 15:e0228895 (2020). PubMed: 32032388
- Deng Z et al. Characterization of articular cartilage homeostasis and the mechanism of superior cartilage regeneration of MRL/MpJ mice. FASEB J N/A:fj201802132RR (2019). PubMed: 31042406
- Krafft U et al. Validation of survivin and HMGA2 as biomarkers for cisplatin resistance in bladder cancer. Urol Oncol N/A:N/A (2019). PubMed: 31053526
- Farrelly LA et al. Histone serotonylation is a permissive modification that enhances TFIID binding to H3K4me3. Nature 567:535-539 (2019). PubMed: 30867594
- Qin XY et al. Arachidonic acid suppresses hepatic cell growth through ROS-mediated activation of transglutaminase. FEBS Open Bio 8:1703-1710 (2018). PubMed: 30338220
