Key features and details
- Rabbit polyclonal to Transglutaminase 2
- Suitable for: WB, ELISA, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Guinea pig, Human
- Isotype: IgG
参阅全部 Transglutaminase 2 一抗
描述兔多克隆抗体to Transglutaminase 2
特异性This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4).
经测试应用适用于: WB, ELISA, IHC-P, IHC-Fr, ICC/IFmore details
种属反应性与反应: Mouse, Rat, Guinea pig, Human
Full length native protein (purified) (Guinea Pig).
- IHC-P: liver sections WB: human fibroblasts (see review), guinea pig liver lysate
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/5000.|
|ELISA||1/300. 1/300 (See Abreview).|
|IHC-P||1/50 - 1/500.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21359202|
功能Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
序列相似性Belongs to the transglutaminase superfamily. Transglutaminase family.
- Information by UniProt
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 220.127.116.11 antibody
ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immnohistochemical analysis of murine intestine tissue treated with poly(I:C), staining Transglutaminase 2 with ab421.
Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 for 10 min at RT, then blocked in 0.3% Triton X-100 and 5% goat serum in PBS. Samples were incubated with primary antibody (1/250) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.
ab421 被引用在 22 文献中.
- Yu W et al. Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway. Lab Invest N/A:N/A (2019). PubMed: 30683901
- Dib A et al. In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring. Front Physiol 9:350 (2018). PubMed: 29670546
- Murray-Stewart T et al. Polyamine Homeostasis in Snyder-Robinson Syndrome. Med Sci (Basel) 6:N/A (2018). PubMed: 30544565
- Kuwahara G et al. CD44 Promotes Inflammation and Extracellular Matrix Production During Arteriovenous Fistula Maturation. Arterioscler Thromb Vasc Biol 37:1147-1156 (2017). PubMed: 28450292
- Pandya CD et al. Transglutaminase 2 overexpression induces depressive-like behavior and impaired TrkB signaling in mice. Mol Psychiatry 22:745-753 (2017). IHC . PubMed: 27620841