Key features and details
- Rabbit polyclonal to Transglutaminase 2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
参阅全部 Transglutaminase 2 一抗
描述兔多克隆抗体to Transglutaminase 2
特异性This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4).
经测试应用适用于: ICC/IFmore details
Full length native protein (purified) (Guinea Pig).
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Constituent: Whole serum
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功能Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
序列相似性Belongs to the transglutaminase superfamily. Transglutaminase family.
- Information by UniProt
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 126.96.36.199 antibody
ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab421 被引用在 25 文献中.
- Sheftel CM & Hernandez LL Serotonin stimulated parathyroid hormone related protein induction in the mammary epithelia by transglutaminase-dependent serotonylation. PLoS One 15:e0241192 (2020). PubMed: 33095824
- Yang P et al. TGM2 interference regulates the angiogenesis and apoptosis of colorectal cancer via Wnt/ß-catenin pathway. Cell Cycle 18:1122-1134 (2019). PubMed: 31010374
- Yu W et al. Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway. Lab Invest N/A:N/A (2019). PubMed: 30683901
- Dib A et al. In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring. Front Physiol 9:350 (2018). PubMed: 29670546
- Murray-Stewart T et al. Polyamine Homeostasis in Snyder-Robinson Syndrome. Med Sci (Basel) 6:N/A (2018). PubMed: 30544565