Key features and details
- Rabbit polyclonal to Transglutaminase 2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
参阅全部 Transglutaminase 2 一抗
描述兔多克隆抗体to Transglutaminase 2
特异性This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4).
经测试应用适用于: ICC/IFmore details
Full length native protein (purified) (Guinea Pig).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
功能Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
序列相似性Belongs to the transglutaminase superfamily. Transglutaminase family.
- Information by UniProt
- ALPHA SUBUNIT antibody
- C polypeptide antibody
- EC 18.104.22.168 antibody
ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab421 被引用在 24 文献中.
- Yu W et al. Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway. Lab Invest N/A:N/A (2019). PubMed: 30683901
- Yang P et al. TGM2 interference regulates the angiogenesis and apoptosis of colorectal cancer via Wnt/ß-catenin pathway. Cell Cycle 18:1122-1134 (2019). PubMed: 31010374
- Murray-Stewart T et al. Polyamine Homeostasis in Snyder-Robinson Syndrome. Med Sci (Basel) 6:N/A (2018). PubMed: 30544565
- Png E et al. Mechanistic role of transglutaminase-2 in focal adhesions. Sci Rep 8:12370 (2018). PubMed: 30120307
- Dib A et al. In Utero Exposure to Maternal Diabetes Is Associated With Early Abnormal Vascular Structure in Offspring. Front Physiol 9:350 (2018). PubMed: 29670546