Key features and details
- Mouse monoclonal [OX26] to Transferrin Receptor
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Rat
- Isotype: IgG2a
参阅全部 Transferrin Receptor 一抗
描述小鼠单克隆抗体[OX26] to Transferrin Receptor
经测试应用适用于: Flow Cyt, IHC-Pmore details
Tissue, cells or virus corresponding to Transferrin Receptor.
- Flow Cyt: Lewis rat bone marrow cells. IHC-P: Mouse and rat placenta tissue.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Protein G purified
Our Abpromise guarantee covers the use of ab6331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 0.2 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
功能Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
序列相似性Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain.
翻译后修饰N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
细胞定位Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
Lewis rat bone marrow cells stained with ab6331 (right) or mouse IgG2a kappa (ab170191) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6331) or mouse IgG2a kappa (ab170191) isotype (1x 106 in 100 µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ®488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice.
The cells were simultaneously stained with Ter-119.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live single cells.
IHC image of Transferrin receptor staining in a section of formalin-fixed paraffin-embedded normal rat placenta performed on a Leica BOND™ system using the standard Protocol F, omitting the post-primary step. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab6331, 5µg/ml, for 15 mins at room temperature. The section was then incubated with ab98792 (Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L, pre-adsorbed) 1/500 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
IHC image of Transferrin receptor staining in normal mouse placenta formalin fixed paraffin embedded tissue section, using Mouse on Mouse detection kit ab127055. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab6331, 5µg/ml overnight at +4°C. The section was counterstained with hematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.