Application
Flow Cytometry
Fixation
None
Permeabilization
No
Sample
Human Cell (PC-3 Prostrate cancer cell line)
Specification
PC-3 Prostrate cancer cell line
Gating Strategy
Cells gated for live and singlets
Preparation
Cell harvesting/tissue preparation method: Cells detached with PBS-based cell dissociation buffer, collected by centrifugation and re-suspended in PBS + 2% FBS
Sample buffer: PBS + 2% FBS
Sample buffer: PBS + 2% FBS
Other product details
Concentration
0.3 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 2% FBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
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Abcam user community
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提交于 Nov 05 2013