Key features and details
- Rabbit polyclonal to Transducin alpha
- Suitable for: WB
- Reacts with: Mouse, Sheep, Cow, Pig
- Isotype: IgG
参阅全部 GNAT1 一抗
描述兔多克隆抗体to Transducin alpha
特异性Detects Rod Transducin alpha from bovine outer rod segments and sheep retinal extract.
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Sheep, Cow, Pig
预测可用于: Rat, Dog, Human, Xenopus laevis
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
纯度Immunogen affinity purified
Primary antibody说明Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.
Our Abpromise guarantee covers the use of ab3504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 40 kDa).|
功能Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
组织特异性Rod. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium.
疾病相关Night blindness, congenital stationary, autosomal dominant 3
Night blindness, congenital stationary, 1C
序列相似性Belongs to the G-alpha family. G(i/o/t/z) subfamily.
发展阶段Expressed at approximately postnatal day 7.
- Information by UniProt
- CSNBAD3 antibody
- GBT1 antibody
- GNAT1 antibody
ab3504 被引用在 2 文献中.
- Taniuchi K et al. Overexpression of GalNAc-transferase GalNAc-T3 promotes pancreatic cancer cell growth. Oncogene 30:4843-54 (2011). PubMed: 21625220
- Adamus G et al. Molecular biomarkers for autoimmune retinopathies: significance of anti-transducin-alpha autoantibodies. Exp Mol Pathol 87:195-203 (2009). PubMed: 19744478