概述

  • 产品名称
  • 描述
    兔多克隆抗体to TRAIL
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ELISA, Neutralising, WB, IHC-P, ICC/IF, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human TRAIL aa 114-281. Highly pure (>98%). E.coli derived
    Sequence:

    MRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSF LSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKY TSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNE HLIDMDHEASFFGAFLVG


    Database link: P50591

  • 阳性对照
    • Recombinant human TRAIL protein (ab9960) can be used as a positive control in WB. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDA-MB-231

性能

应用

Our Abpromise guarantee covers the use of ab9959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. To detect hTRAIL by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTRAIL.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL (30 ng/ml), a concentration of 0.5 - 0.8 mg/ml of this antibody is required.
WB Use at an assay dependent concentration. To detect hTRAIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
Sandwich ELISA Use a concentration of 0.1 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.1µg/ml with ab12124 as Capture.

靶标

  • 功能
    Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
  • 组织特异性
    Widespread; most predominant in spleen, lung and prostate.
  • 序列相似性
    Belongs to the tumor necrosis factor family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apo 2 ligand antibody
    • APO 2L antibody
    • Apo-2 ligand antibody
    • Apo-2L antibody
    • APO2L antibody
    • CD253 antibody
    • CD253 antigen antibody
    • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
    • Protein TRAIL antibody
    • TL2 antibody
    • TNF Related Apoptosis Inducing Ligand antibody
    • TNF related apoptosis inducing ligand TRAIL antibody
    • TNF-related apoptosis-inducing ligand antibody
    • TNF10_HUMAN antibody
    • TNFSF10 antibody
    • TRAIL antibody
    • Tumor necrosis factor (ligand) family member 10 antibody
    • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
    • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
    • Tumor necrosis factor ligand superfamily member 10 antibody
    see all

图片

  • ab9959 staining TRAIL in human metastatic carcinoma of lymph nodes from breast tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ICC/IF image of ab9959 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9959 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab9959 staining TRAIL in human metastatic carcinoma of lymph nodes from breast tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
  • Standard curve for TRAIL (Analyte: ab78818); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [55B709.3] to TRAIL (ab12124) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to TRAIL (ab9959) at 0.1µg/ml.

文献

This product has been referenced in:
  • He X  et al. An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes. Nucleic Acids Res 43:3712-25 (2015). IHC-P . Read more (PubMed: 25779046) »
  • Graves JD  et al. Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activity. Cancer Cell 26:177-89 (2014). WB ; Human . Read more (PubMed: 25043603) »
See all 2 Publications for this product

客户评价及客户问答

Answer

Thank you for your enquiry. I am pleased to help answer your questions. 1. I am sorry to confirm that ab12124 antibody would not be suitable for detection of the ab78818 protein. As you have noticed from the different amino acid sequences, they are not designed to be used together. 2. ab9959 is raised against full length protein. As a polyclonal antibody, it will be detecting epitopes along the entire length of the protein. However, we could not confirm or guarantee that it would detect the shorter length recombinant ab78818 as the epitopes will not have been mapped. i would suggest to use a full length recombinant protein. I am sorry we do not have full length protein in the catalogue at this time. I am sorry there are no suitable protein standards to suggest on this occasion for use with both these antibodies together. If you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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