Anti-TRAIL抗体(ab2056)

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Key features and details

  • Rabbit polyclonal to TRAIL
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-TRAIL抗体
    参阅全部 TRAIL 一抗

  • 描述

    兔多克隆抗体to TRAIL

  • 宿主

    Rabbit

  • 特异性

    Human.

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human TRAIL aa 261-277 (C terminal).
    Sequence:

    CTNEHLIDMDHEASFFGA


    (Peptide available as ab7867)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • HeLa whole cell lysate.

  • 常规说明

    Ligand for DR4 and DR5.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab2056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 20 µg/ml.
WB 1/500. Detects a band of approximately 32 kDa.Can be blocked with TRAIL peptide (ab7867).

Gives a 32 kD band which represents the membrane-bound form of TRAIL.

靶标

  • 功能

    Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

  • 组织特异性

    Widespread; most predominant in spleen, lung and prostate.

  • 序列相似性

    Belongs to the tumor necrosis factor family.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Apo 2 ligand antibody
      • APO 2L antibody
      • Apo-2 ligand antibody
      • Apo-2L antibody
      • APO2L antibody
      • CD253 antibody
      • CD253 antigen antibody
      • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
      • Protein TRAIL antibody
      • TL2 antibody
      • TNF Related Apoptosis Inducing Ligand antibody
      • TNF related apoptosis inducing ligand TRAIL antibody
      • TNF-related apoptosis-inducing ligand antibody
      • TNF10_HUMAN antibody
      • TNFSF10 antibody
      • TRAIL antibody
      • Tumor necrosis factor (ligand) family member 10 antibody
      • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
      • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
      • Tumor necrosis factor ligand superfamily member 10 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human brain tissue staining TRAIL with ab2056 at 20µg/ml
    • Western blot - Anti-TRAIL antibody (ab2056)
      Western blot - Anti-TRAIL antibody (ab2056)

      Western Blot of human brain lysate labeling TRAIL with Anti-TRAIL antibody (ab2056) at (1) 2.5 and (2) 5 µg/ml.

    • Western blot - Anti-TRAIL antibody (ab2056)
      Western blot - Anti-TRAIL antibody (ab2056)
      Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein including extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab2056)
      Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 µg/ml.

    文献 (3)

    发表研究结果有使用 ab2056？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2056 被引用在 3 文献中.

    • Melendez ME  et al. Construction and characterization of a new TRAIL soluble form, active at picomolar concentrations. Oncotarget 9:27233-27241 (2018). PubMed: 29930761
    • Ludwig S  et al. Suppression of Lymphocyte Functions by Plasma Exosomes Correlates with Disease Activity in Patients with Head and Neck Cancer. Clin Cancer Res 23:4843-4854 (2017). PubMed: 28400428
    • Kawano Y  et al. TRAIL produced from multiple myeloma cells is associated with osteolytic markers. Oncol Rep 27:39-44 (2012). PubMed: 21993926

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Question

    Do you sell a rabbit polyclonalTRAIL antibody that recognizes an epitope between amino acid 95 and the C-terminal, that reacts with human for use in WB?

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    Answer

    Thank you for contacting us. We currently sell the following antibodies that meet your description:

    ab2435 - immunogen: C-terminal; tested species: mouse, human; tested applications: IHC-P, IHC-Fr, IP, WB, ICC/IF

    ab42121 - immunogen: aa 250 - C-terminal; tested species: human; tested applications: IHC-P, WB

    ab2056 - immunogen: aa 261 - 277; tested species: human; tested applications: IHC-P, WB

    ab46953 - immunogen: aa 133 - 144; tested species: human; tested applications: WB

    I hope this helps, please let me know if you need any additional information.

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    Question

    I was curious if you can provide me with a typical western blot image. Can you show me images againts full length human TRAIL protein? Where should I normally expect a band, 32kda or 24kda? Also, can you suggest what lysis buffer should be used for TRAIL WB, I was using the NP-40 lysis buffer, but the protein yield is very poor, what buffer do you recommend?

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    Answer

    Thank you for your enquiry. Ab2056 recognizes both the whole TRAIL protein (32 kDa) as well as its extracellular domain (19 kDa). If you are running a Western with HeLa whole cell lysate (which is recommended for a positive control) you would expect to see a band at 32 kDa. I will email you an image separately. If you are running recombinant TRAIL protein, you would see a band at 24 kDa - Recombinant human TRAIL monomer has a molecular mass of approximately 21 kDa. Due to glycosylation, recombinant human TRAIL migrates as a 24 kDa protein in SDS PAGE. The originator of this antibody used the following for the lysis buffer: HeLa cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. If you have any additional questions, please contact us again.

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    Question

    Does this antibody recognises native Human trail in reducing gels?

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    Answer

    Yes, the antibody does recognise native human TRAIL in reducing and non-reducing gels.

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