Anti-TRAIL抗体(ab2056)
Key features and details
- Rabbit polyclonal to TRAIL
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRAIL抗体
参阅全部 TRAIL 一抗 -
描述
兔多克隆抗体to TRAIL -
宿主
Rabbit -
特异性
Human. -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
免疫原
Synthetic peptide corresponding to Human TRAIL aa 261-277 (C terminal).
Sequence:CTNEHLIDMDHEASFFGA
(Peptide available asab7867) -
阳性对照
- HeLa whole cell lysate.
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常规说明
Ligand for DR4 and DR5.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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IHC-P |
Human
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WB |
Human
|
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use a concentration of 20 µg/ml.
|
|
WB |
1/500. Detects a band of approximately 32 kDa.Can be blocked with TRAIL peptide (ab7867).
Gives a 32 kD band which represents the membrane-bound form of TRAIL. |
说明 |
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IHC-P
Use a concentration of 20 µg/ml. |
WB
1/500. Detects a band of approximately 32 kDa.Can be blocked with TRAIL peptide (ab7867). Gives a 32 kD band which represents the membrane-bound form of TRAIL. |
靶标
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功能
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
组织特异性
Widespread; most predominant in spleen, lung and prostate. -
序列相似性
Belongs to the tumor necrosis factor family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8743 Human
- Omim: 603598 Human
- SwissProt: P50591 Human
- Unigene: 478275 Human
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别名
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human brain tissue staining TRAIL with ab2056 at 20µg/ml
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Western Blot of human brain lysate labeling TRAIL with Anti-TRAIL antibody (ab2056) at (1) 2.5 and (2) 5 µg/ml.
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Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein including extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml Western blot analysis of HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with anti-TRAIL at 1 µg/ml.
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Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab2056 被引用在 3 文献中.
- Melendez ME et al. Construction and characterization of a new TRAIL soluble form, active at picomolar concentrations. Oncotarget 9:27233-27241 (2018). PubMed: 29930761
- Ludwig S et al. Suppression of Lymphocyte Functions by Plasma Exosomes Correlates with Disease Activity in Patients with Head and Neck Cancer. Clin Cancer Res 23:4843-4854 (2017). PubMed: 28400428
- Kawano Y et al. TRAIL produced from multiple myeloma cells is associated with osteolytic markers. Oncol Rep 27:39-44 (2012). PubMed: 21993926