Anti-TRAIL抗体[2E5] (ab2219)

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Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)

    Key features and details

    • Mouse monoclonal [2E5] to TRAIL
    • Suitable for: Functional Studies
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-TRAIL抗体[2E5]
      参阅全部 TRAIL 一抗

    • 描述

      小鼠单克隆抗体[2E5] to TRAIL

    • 宿主

      Mouse

    • 特异性

      This antibody is specific for TRAIL, Tumour necrosis factor (TNF) related apoptosis inducing ligand (TRAIL/ APO2-L)).

    • 经测试应用

      适用于: Functional Studiesmore details

    • 种属反应性

      与反应: Human
      不与反应: Mouse

    • 免疫原

      Recombinant soluble fragment, corresponding to amino acids 95-281 of Human TRAIL.

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab2219 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.

    靶标

    • 功能

      Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

    • 组织特异性

      Widespread; most predominant in spleen, lung and prostate.

    • 序列相似性

      Belongs to the tumor necrosis factor family.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Apo 2 ligand antibody
        • APO 2L antibody
        • Apo-2 ligand antibody
        • Apo-2L antibody
        • APO2L antibody
        • CD253 antibody
        • CD253 antigen antibody
        • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
        • Protein TRAIL antibody
        • TL2 antibody
        • TNF Related Apoptosis Inducing Ligand antibody
        • TNF related apoptosis inducing ligand TRAIL antibody
        • TNF-related apoptosis-inducing ligand antibody
        • TNF10_HUMAN antibody
        • TNFSF10 antibody
        • TRAIL antibody
        • Tumor necrosis factor (ligand) family member 10 antibody
        • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
        • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
        • Tumor necrosis factor ligand superfamily member 10 antibody
        see all

      图片

      • Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)
        Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)
        Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5).
        The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL.
        A - medium
        B - recombinant TRAIL
        C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 µg/ml)
        D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 µg/ml)
        E - recombinant TRAIL + Isotype mouse IgG1 control

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      文献 (5)

      发表研究结果有使用 ab2219？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab2219 被引用在 5 文献中.

      • Tatzel K  et al. Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology. Sci Rep 6:22661 (2016). Flow Cyt . PubMed: 26935795
      • Yunker PJ  et al. One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces. Proc Natl Acad Sci U S A 113:608-13 (2016). PubMed: 26721399
      • Sharova TY  et al. Complex Changes in the Apoptotic and Cell Differentiation Programs during Initiation of the Hair Follicle Response to Chemotherapy. J Invest Dermatol N/A:N/A (2014). PubMed: 24999588
      • Hyer ML  et al. Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res 65:4799-808 (2005). PubMed: 15930300
      • Plasilova M  et al. TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia 16:67-73 (2002). PubMed: 11840265

      客户评价及客户问答

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      Question

      I would like to know if this antibody is an agonist vs antagonist of TRAIL receptor. You mentioned functional studies on the datasheet. Many thanks,

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      Answer

      This antibody was raised against soluble recombinant TRAIL /APO-2L (extracellular domain). It completely neutralizes apoptosis in cells induced by TRAIL (e.g. by soluble recombinant human TRAIL) that is why datasheet states functional application.

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      Question

      send functional study data for ab2219

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      Answer

      Thank you for contacting us.
      I have attached the data for the Functional Studies done with ab2219. We will add these to the datasheet as well very soon.
      Also, I wanted to send you the information about our 3 for 2 promotion for all proteins that is going on until the end of the month.
      https://www.abcam.com/index.html?pageconfig=resource&rid=15079
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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