Anti-TRAIL抗体[2E5] (ab2219)

Submit a review Q&A (2)References (5)

Key features and details

  • Mouse monoclonal [2E5] to TRAIL
  • Suitable for: Functional Studies, Flow Cyt, Neutralising
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-TRAIL抗体[2E5]
    参阅全部 TRAIL 一抗

  • 描述

    小鼠单克隆抗体[2E5] to TRAIL

  • 宿主

    Mouse

  • 特异性

    This antibody is specific for TRAIL, Tumour necrosis factor (TNF) related apoptosis inducing ligand (TRAIL/ APO2-L)).

  • 经测试应用

    适用于: Functional Studies, Flow Cyt, Neutralisingmore details

  • 种属反应性

    与反应: Human
    不与反应: Mouse

  • 免疫原

    Recombinant soluble fragment, corresponding to amino acids 95-281 of Human TRAIL.

性能

应用

Our Abpromise guarantee covers the use of ab2219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.
Flow Cyt Use a concentration of 1 - 10 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Neutralising Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.

靶标

  • 功能

    Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

  • 组织特异性

    Widespread; most predominant in spleen, lung and prostate.

  • 序列相似性

    Belongs to the tumor necrosis factor family.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Apo 2 ligand antibody
      • APO 2L antibody
      • Apo-2 ligand antibody
      • Apo-2L antibody
      • APO2L antibody
      • CD253 antibody
      • CD253 antigen antibody
      • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
      • Protein TRAIL antibody
      • TL2 antibody
      • TNF Related Apoptosis Inducing Ligand antibody
      • TNF related apoptosis inducing ligand TRAIL antibody
      • TNF-related apoptosis-inducing ligand antibody
      • TNF10_HUMAN antibody
      • TNFSF10 antibody
      • TRAIL antibody
      • Tumor necrosis factor (ligand) family member 10 antibody
      • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
      • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
      • Tumor necrosis factor ligand superfamily member 10 antibody
      see all

    图片

    • Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)
      Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)
      Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5).
      The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL.
      A - medium
      B - recombinant TRAIL
      C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 µg/ml)
      D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 µg/ml)
      E - recombinant TRAIL + Isotype mouse IgG1 control

    文献 (5)

    发表研究结果有使用 ab2219？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab2219 被引用在 5 文献中.

    • Tatzel K  et al. Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology. Sci Rep 6:22661 (2016). Flow Cyt . PubMed: 26935795
    • Yunker PJ  et al. One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces. Proc Natl Acad Sci U S A 113:608-13 (2016). PubMed: 26721399
    • Sharova TY  et al. Complex Changes in the Apoptotic and Cell Differentiation Programs during Initiation of the Hair Follicle Response to Chemotherapy. J Invest Dermatol N/A:N/A (2014). PubMed: 24999588
    • Hyer ML  et al. Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res 65:4799-808 (2005). PubMed: 15930300
    • Plasilova M  et al. TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia 16:67-73 (2002). PubMed: 11840265

    客户评价及客户问答

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    Question

    I would like to know if this antibody is an agonist vs antagonist of TRAIL receptor. You mentioned functional studies on the datasheet. Many thanks,

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    Answer

    This antibody was raised against soluble recombinant TRAIL /APO-2L (extracellular domain). It completely neutralizes apoptosis in cells induced by TRAIL (e.g. by soluble recombinant human TRAIL) that is why datasheet states functional application.

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    Question

    send functional study data for ab2219

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    Answer

    Thank you for contacting us.
    I have attached the data for the Functional Studies done with ab2219. We will add these to the datasheet as well very soon.
    Also, I wanted to send you the information about our 3 for 2 promotion for all proteins that is going on until the end of the month.
    https://www.abcam.com/index.html?pageconfig=resource&rid=15079
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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