Anti-TRAIL抗体[2E5] (ab2219)
![Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)](https://www.abcam.cn/ps/products/2/ab2219/Images/ab2219-5477-anti-trail-antibody-2e5.jpg "Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)")
Key features and details
- Mouse monoclonal [2E5] to TRAIL
- Suitable for: Functional Studies
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-TRAIL抗体[2E5]
参阅全部 TRAIL 一抗 -
描述
小鼠单克隆抗体[2E5] to TRAIL -
宿主
Mouse -
特异性
This antibody is specific for TRAIL, Tumour necrosis factor (TNF) related apoptosis inducing ligand (TRAIL/ APO2-L)).
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经测试应用
适用于: Functional Studiesmore details -
种属反应性
与反应: Human
不与反应: Mouse -
免疫原
Recombinant soluble fragment, corresponding to amino acids 95-281 of Human TRAIL.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
2E5 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays. |
靶标
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功能
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
组织特异性
Widespread; most predominant in spleen, lung and prostate. -
序列相似性
Belongs to the tumor necrosis factor family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8743 Human
- Omim: 603598 Human
- SwissProt: P50591 Human
- Unigene: 478275 Human
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别名
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
图片
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Functional Studies - Anti-TRAIL antibody [2E5] (ab2219)Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5).
The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL.
A - medium
B - recombinant TRAIL
C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 µg/ml)
D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 µg/ml)
E - recombinant TRAIL + Isotype mouse IgG1 control
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab2219 被引用在 6 文献中.
- Nordmaj MA et al. Development of a bispecific immune engager using a recombinant malaria protein. Cell Death Dis 12:353 (2021). PubMed: 33824272
- Tatzel K et al. Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology. Sci Rep 6:22661 (2016). Flow Cyt . PubMed: 26935795
- Yunker PJ et al. One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces. Proc Natl Acad Sci U S A 113:608-13 (2016). PubMed: 26721399
- Sharova TY et al. Complex Changes in the Apoptotic and Cell Differentiation Programs during Initiation of the Hair Follicle Response to Chemotherapy. J Invest Dermatol N/A:N/A (2014). PubMed: 24999588
- Hyer ML et al. Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res 65:4799-808 (2005). PubMed: 15930300
- Plasilova M et al. TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia 16:67-73 (2002). PubMed: 11840265