重组Anti-TRAF4抗体[EPR1729] (ab108991)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1729] to TRAF4
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TRAF4抗体[EPR1729]
参阅全部 TRAF4 一抗 -
描述
兔单克隆抗体[EPR1729] to TRAF4 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat, HeLa and SKBR-3 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR1729 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108991于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
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说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa. |
靶标
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功能
Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. -
组织特异性
Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus. -
序列相似性
Belongs to the TNF receptor-associated factor family. B subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 3 TRAF-type zinc fingers. -
结构域
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains. -
翻译后修饰
Polyubiquitinated, leading to its proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 9618 Human
- Entrez Gene: 22032 Mouse
- Omim: 602464 Human
- SwissProt: Q9BUZ4 Human
- SwissProt: Q61382 Mouse
- Unigene: 8375 Human
- Unigene: 390418 Mouse
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别名
- CART 1 antibody
- CART1 antibody
- cysteine rich domain associated with ring and TRAF domain antibody
see all
图片
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Overlay histogram showing HeLa cells stained with ab108991 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108991, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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All lanes : Anti-TRAF4 antibody [EPR1729] (ab108991) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab108991 被引用在 3 文献中.
- Zhang Z et al. HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARa axis. Mol Ther Nucleic Acids 24:711-727 (2021). PubMed: 33996254
- Wang J et al. Long non-coding RNA DLEU1 promotes cell proliferation of glioblastoma multiforme. Mol Med Rep 20:1873-1882 (2019). PubMed: 31257517
- Zepp JA et al. Cutting edge: TNF receptor-associated factor 4 restricts IL-17-mediated pathology and signaling processes. J Immunol 189:33-7 (2012). PubMed: 22649194