Key features and details
- Goat polyclonal to TRAF1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Protein G purified
Our Abpromise guarantee covers the use of ab129279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 46 kDa.|
|IHC-P||Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
功能Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.
序列相似性Contains 1 MATH domain.
结构域The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains.
Cleavage by CASP8 liberates a C-terminal fragment that promotes apoptosis and inhibits the activation of NF-kappa-B in response to TNF signaling.
翻译后修饰Polyubiquitinated by BIRC2 and/or BIRC3, leading to its subsequent proteasomal degradation.
- Information by UniProt
- 4732496E14Rik antibody
- EBI6 antibody
- EBV induced protein 6 antibody
Anti-TRAF1 antibody (ab129279) at 0.5 µg/ml (for 1 hour) + U937 cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
ab129279, at 3.75 µg/ml, staining TRAF1 in Formalin-fixed, Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
ab129279, at 3.75 µg/ml, staining TRAF1 in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry.
ab129279 被引用在 1 文献中.
- Lu YY et al. TRAF1 is a critical regulator of cerebral ischaemia-reperfusion injury and neuronal death. Nat Commun 4:2852 (2013). IHC-Fr ; Mouse . PubMed: 24284943