Anti-TRAF1抗体(ab129279)
Key features and details
- Goat polyclonal to TRAF1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-TRAF1抗体
参阅全部 TRAF1 一抗 -
描述
山羊多克隆抗体to TRAF1 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Chicken, Cow, Dog, Zebrafish, a wide range of other species
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免疫原
Synthetic peptide:
KLQSPKHAYVKDD
, corresponding to C terminal amino acids 392-404 of Human TRAF1 (NP_005649.1). -
阳性对照
- Human Small Intestine and Thymus tissues; U937 cell lysate.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab129279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 46 kDa. | |
| IHC-P | Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. -
序列相似性
Contains 1 MATH domain. -
结构域
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains.
Cleavage by CASP8 liberates a C-terminal fragment that promotes apoptosis and inhibits the activation of NF-kappa-B in response to TNF signaling. -
翻译后修饰
Polyubiquitinated by BIRC2 and/or BIRC3, leading to its subsequent proteasomal degradation. - Information by UniProt
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数据库链接
- Entrez Gene: 7185 Human
- Entrez Gene: 22029 Mouse
- Omim: 601711 Human
- SwissProt: Q13077 Human
- SwissProt: P39428 Mouse
- Unigene: 531251 Human
- Unigene: 239514 Mouse
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别名
- 4732496E14Rik antibody
- EBI6 antibody
- EBV induced protein 6 antibody
see all
图片
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Western blot - Anti-TRAF1 antibody (ab129279)Anti-TRAF1 antibody (ab129279) at 0.5 µg/ml (for 1 hour) + U937 cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 46 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (ab129279)ab129279, at 3.75 µg/ml, staining TRAF1 in Formalin-fixed, Paraffin-embedded Human small intestine tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (ab129279)ab129279, at 3.75 µg/ml, staining TRAF1 in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry.
数据表及文件
文献
ab129279 被引用在 1 文献中.
- Lu YY et al. TRAF1 is a critical regulator of cerebral ischaemia-reperfusion injury and neuronal death. Nat Commun 4:2852 (2013). IHC-Fr ; Mouse . PubMed: 24284943
