重组Anti-TPN抗体[EPR25083-20] (ab288565)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25083-20] to TPN
- Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-TPN抗体[EPR25083-20]
参阅全部 TPN 一抗 -
描述
兔单克隆抗体[EPR25083-20] to TPN -
宿主
Rabbit -
特异性
This product is not suitable for IHC with samples from mouse.
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经测试应用
适用于: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Untreated HeLa, HeLa treated with IFN gamma for 16 hours, B16-F10 treated with IFN gamma for 16 hours, Mouse spleen, Human liver, RAW264.7, HeLa, A431 and Jurkat lysates. IHC-P: Human colon, Human lung, Human colon carcinoma and Human lung adenocarcinoma tissues. ICC: HeLa and Jurkat cells. Flow Cyt: Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR25083-20 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab288565于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 48 kDa.
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IHC-P |
1/500.
This product is not suitable for IHC with samples from mouse. |
|
Flow Cyt (Intra) |
1/500.
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ICC/IF |
1/500.
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说明 |
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WB
1/1000. Predicted molecular weight: 48 kDa. |
IHC-P
1/500. This product is not suitable for IHC with samples from mouse. |
Flow Cyt (Intra)
1/500. |
ICC/IF
1/500. |
靶标
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功能
Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading). -
组织特异性
Neutrophils, mostly in fully differentiated cells. -
序列相似性
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
结构域
The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminus region. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6892 Human
- Entrez Gene: 21356 Mouse
- Omim: 601962 Human
- SwissProt: O15533 Human
- SwissProt: Q9R233 Mouse
- Unigene: 370937 Human
- Unigene: 154457 Mouse
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别名
- NGS 17 antibody
- NGS-17 antibody
- NGS17 antibody
see all
图片
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All lanes : Anti-TPN antibody [EPR25083-20] (ab288565) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
Lane 2 : HeLa treated with 100 ng/ml IFN gamma for 16 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 48 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Upregulating the expression of TPN protein by IFN gamma treatment (PMID: 11169257)
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All lanes : Anti-TPN antibody [EPR25083-20] (ab288565) at 1/1000 dilution
Lane 1 : Untreated B16-F10 (Mouse skin melanoma), whole cell lysate
Lane 2 : B16-F10 treated with 100 ng/ml IFN gamma for 16 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 48 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Upregulating the expression of TPN protein by IFN gamma treatment (PMID: 11169257)
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All lanes : Anti-TPN antibody [EPR25083-20] (ab288565) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate
Lane 5 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
Lane 6 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 48 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure times:
Lanes 1 & 2: 48 seconds
Lane 3: 3 minutes
Lane 4: 26 seconds
Laned 5 & 6: 15 seconds
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TPN with ab288565 at 1/500 (1.002 ug/ml) followed by ready to use Leica DS9800 (BOND™, Polymer Refine Detection). Cytoplasmic staining on the stromal cells of human colon (PMID: 26310568). The section was incubated with ab288565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Ready to use Leica DS9800 (BOND™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling TPN with ab288565 at 1/500 (1.002 ug/ml) followed by ready to use Leica DS9800 (BOND™, Polymer Refine Detection). Mainly cytoplasmic staining on the leukocytes of human lung. The section was incubated with ab288565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Ready to use Leica DS9800 (BOND™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labelling TPN with ab288565 at 1/500 (1.002 ug/ml) followed by ready to use Leica DS9800 (BOND™, Polymer Refine Detection). Cytoplasmic staining on the cancer cells and stromal cells of human colon carcinoma (PMID: 26310568). The section was incubated with ab288565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: ready to use Leica DS9800 (BOND™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labelling TPN with ab288565 at 1/500 (1.002 ug/ml) followed by a ready to use Leica DS9800 (BOND™, Polymer Refine Detection) was used. Cytoplasmic staining on the cancer cells and stromal cells of human lung adenocarcinoma. The section was incubated with ab288565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: ready to use Leica DS9800 (BOND™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling TPN with ab288565 at 1/500 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat cells labelling TPN with ab288565 at 1/500 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Jurkat cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488), preadsorbed, at 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling TPN with ab288565 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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