重组Anti-Tissue Plasminogen活化剂抗体[EPR7232(2)] (ab157469)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7232(2)] to Tissue Plasminogen Activator
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Tissue Plasminogen活化剂抗体[EPR7232(2)]
参阅全部 Tissue Plasminogen Activator 一抗 -
描述
兔单克隆抗体[EPR7232(2)] to组织Plasminogen活化剂 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse plasma, Rat plasma, Human plasma, and Human liver lysates; IHC-P: human lung carcinoma, cervical carcinoma, skeletal muscle, brain, kidney and liver tissue, mouse and rat liver tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7232(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab157469于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.
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IHC-P |
1/100 - 1/1000.
Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). |
说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 63 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). |
靶标
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功能
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration. -
组织特异性
Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. -
疾病相关
Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
结构域
Both FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site. -
翻译后修饰
The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line. -
细胞定位
Secreted > extracellular space. - Information by UniProt
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数据库链接
- Entrez Gene: 5327 Human
- Entrez Gene: 18791 Mouse
- Entrez Gene: 25692 Rat
- Omim: 173370 Human
- SwissProt: P00750 Human
- SwissProt: P11214 Mouse
- SwissProt: P19637 Rat
- Unigene: 491582 Human
see all -
别名
- Alteplase antibody
- DKFZp686I03148 antibody
- Plasminogen activator tissue antibody
see all
图片
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All lanes : Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469) at 1/1000 dilution (Purified)
Lane 1 : Mouse plasma lysates
Lane 2 : Rat plasma lysates
Lane 3 : Human plasma lysates
Lane 4 : Human brain lysates
Lane 5 : Human heart lysates
Lane 6 : Human liver lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?T-PA is serected and mainly released into blood and extracellular fluid to activate plasminogen. The molecular weight observed is consistent with what has been described in PMID: 22069469
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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ab157469 (unpurified) showing +ve staining in Human normal liver.
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ab157469 (unpurified) showing +ve staining in Human normal kidney.
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ab157469 (unpurified) showing +ve staining in Human normal brain.
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ab157469 (unpurified) showing +ve staining in Human skeletal muscle.
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Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469) at 1/1000 dilution (unpurified) + Human plasma lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 63 kDa -
Immunohistochemical analysis of paraffin-embedded Human cervial carcinoma tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) at 1/100 dilution.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab157469 被引用在 9 文献中.
- Lottin M et al. Molecular Landscape of the Coagulome of Oral Squamous Cell Carcinoma. Cancers (Basel) 14:N/A (2022). PubMed: 35053621
- Dong Z et al. Paradoxical development of pleural-based masses in patients with pleural tuberculosis during treatment: a clinical observational study in China. BMC Pulm Med 22:126 (2022). PubMed: 35379218
- Chen Z et al. Clinical Relevance of Plasma Endogenous Tissue-Plasminogen Activator and Aortic Valve Sclerosis: Performance as a Diagnostic Biomarker. Front Cardiovasc Med 7:584998 (2020). PubMed: 33173789
- Cheng X et al. Prothrombotic effects of high uric acid in mice via activation of MEF2C-dependent NF-?B pathway by upregulating let-7c. Aging (Albany NY) 12:17976-17989 (2020). PubMed: 32960786
- Shu J et al. Andrographolide protects against endothelial dysfunction and inflammatory response in rats with coronary heart disease by regulating PPAR and NF-?B signaling pathways. Ann Palliat Med 9:1965-1975 (2020). PubMed: 32692217
- Sun F et al. From clinic to mechanism: Proteomics-based assessment of angiogenesis in adrenal pheochromocytoma. J Cell Physiol 234:22057-22070 (2019). PubMed: 31106414
- Cheng M et al. Comprehensive analysis of marker gene detection and computed tomography for the diagnosis of human lung cancer. Oncol Lett 16:4400-4406 (2018). PubMed: 30250539
- Li YB et al. miR-199a-3p is involved in the pathogenesis and progression of diabetic neuropathy through downregulation of SerpinE2. Mol Med Rep 16:2417-2424 (2017). PubMed: 28677735
- Xu K et al. Unique Transcriptional Programs Identify Subtypes of AKI. J Am Soc Nephrol 28:1729-1740 (2017). PubMed: 28028135