Used Pig Skeletal Muscle preps. Loaded 30ug of protein for pig skeletal muscle samples, and 5uL of the Rat Heart Positive Control onto 5-12% gradient gel. Transferred onto nitrocellulose membrane. Blocked with 1XTBST with 5% nonfat dry milk. Antibody was diluted 1:1000 in 1xTBST. Secondary Goat anti Mouse Secondary Antibody (green). After imaging, only picked up 4 bands total in pig muscle (2 very faint).
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提交于 Oct 02 2018