概述

  • 产品名称
  • 描述
    山羊多克隆抗体to TORC2
  • 宿主
    Goat
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    C-HGQQPYHRPIND

    , corresponding to internal sequence amino acids 541-552 of Mouse Torc2.

  • 阳性对照
    • Mouse and Rat brain and spinal cord lysates

性能

应用

Our Abpromise guarantee covers the use of ab115035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).

靶标

  • 功能
    Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
  • 组织特异性
    Most abundantly expressed in the thymus. Present in both B and T lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas.
  • 序列相似性
    Belongs to the TORC family.
  • 翻译后修饰
    Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. Both insulin and AMPK increase this phosphorylation, of TORC2 while glucagon suppresses it.
  • 细胞定位
    Cytoplasm. Nucleus. Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CREB regulated transcription coactivator 2 antibody
    • CREB-regulated transcription coactivator 2 antibody
    • CRTC2 antibody
    • CRTC2_HUMAN antibody
    • RP11-422P24.6 antibody
    • TORC-2 antibody
    • torc2 antibody
    • Transducer of CREB protein 2 antibody
    • Transducer of regulated cAMP response element-binding protein antibody
    • Transducer of regulated cAMP response element-binding protein (CREB) 2 antibody
    • Transducer of regulated cAMP response element-binding protein 2 antibody
    • Transducer of regulated CREB protein 2 antibody
    see all

图片

  • Anti-TORC2 antibody (ab115035) at 0.03 µg/ml + Rat spinal cord lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 73 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

实验方案

文献

ab115035 has not yet been referenced specifically in any publications.

客户评价及客户问答

Answer

Thank you for your reply. We would suggest that procedures will usually require some optimization by the end user. However, I am pleased to provide the following suggestions: For how long can I keep the fixed coverslips before the blocking and incubation with antibody? Should the fixed coverslips be kept in -4 degrees? I would recommend to stain the coverslips as soon as you can after staining. However, they should keep for several weeks at 4oC, in a box containing dehydration crystals to keep the slides dry and protected from condensation. 1. What would be a recommended fixation for this antibody? Would you recommend to try several different fixation solutions? Various fixation methods could be tried. I can suggest to consider using just one of the following, do not mix the fixation solutions or use more than one method on one slide. You can try each the following individually to see which provides optimal results. 4% PFA for 10 minutes only Ice cold acetone at -20oC for 10 minutes only Ice cold methanol at -20oC for 10 minutes only. 2. Do you believe that permeabilization with Triton will be sufficient in this case? Triton is quite a strong detergent, and so should be suitable for permeabilization in this case. A permeabilization step is required only after PFA fixation. If you use a solvents such as acetone and methanol for fixation, this will already permeabilize the cell, and so no permeabilization step would be required in this case. Will the Triton allow permeabilization of nuclei membrane of the cells? Yes. Try 0.1 to 0.2% Triton for 10 minutes only. I can also recommend to include a more gentle detergent, such as 0.2% Tween 20, in the antibody dilution buffer and wash buffer. This will help to keep the cells permeabilized. I can suggest to review the information on our protocols pages on the website. These can be found by clicking on the scientific support tab. Select protocols and troubleshooting from the dropdown menu. Then immunostaining. ICC: https://www.abcam.com/index.html?pageconfig=resource&rid=11417 Fixation and permeabilization: https://www.abcam.com/ps/pdf/protocols/fixation_permeabilization.pdf I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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