Anti-TopBP1抗体(ab2402)
Key features and details
- Rabbit polyclonal to TopBP1
- Suitable for: WB
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
概述
-
产品名称
Anti-TopBP1抗体
参阅全部 TopBP1 一抗 -
描述
兔多克隆抗体to TopBP1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human, African green monkey
预测可用于: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide (Human) - which represents a portion of human Topoisomerase (DNA) II Binding Protein I encoded within exon 28.
-
常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
应用
Our Abpromise guarantee covers the use of ab2402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use a concentration of 0.1 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 173.3 kDa). |
靶标
-
功能
Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. -
组织特异性
Highly expressed in heart, brain, placenta, lung and kidney. -
序列相似性
Contains 8 BRCT domains. -
翻译后修饰
Phosphorylated on serine and threonine residues in response to X-ray irradiation.
Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. -
细胞定位
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. - Information by UniProt
-
数据库链接
- Entrez Gene: 11073 Human
- Entrez Gene: 235559 Mouse
- Omim: 607760 Human
- SwissProt: Q92547 Human
- SwissProt: Q6ZQF0 Mouse
- Unigene: 593379 Human
- Unigene: 259893 Mouse
-
别名
- DNA topoisomerase 2 binding protein 1 antibody
- DNA topoisomerase 2-binding protein 1 antibody
- DNA topoisomerase II binding protein 1 antibody
see all
图片
-
Lane 1: WB Cell Lysate (5% of input IP)
Lane 2: IP control (normal rabbit IgG)
Lane 3: IP/WBConditions:
IP: Rabbit polyclonal to TopBP1 (1µ g)
WB: Rabbit polyclonal to TopBP1 (1/2000 dilution)
Positive control: HeLa CellsLane 1: WB Cell Lysate (5% of input IP)
Lane 2: IP control (normal rabbit IgG)
Lane 3: IP/WBConditions:
IP: Rabbit polyclonal to TopBP1 (1µg)
WB: Rabbit polyclonal to TopBP1 (1/2000 dilution)
Positive control: HeLa Cells
文献 (32)
ab2402 被引用在 32 文献中.
- Mooser C et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
- Kidiyoor GR et al. ATR is essential for preservation of cell mechanics and nuclear integrity during interstitial migration. Nat Commun 11:4828 (2020). PubMed: 32973141
- Deshar R et al. RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling. Nucleic Acids Res 47:762-778 (2019). PubMed: 30445466
- Wu M et al. A PLK1 kinase inhibitor enhances the chemosensitivity of cisplatin by inducing pyroptosis in oesophageal squamous cell carcinoma. EBioMedicine 41:244-255 (2019). PubMed: 30876762
- Michelena J et al. Basal CHK1 activity safeguards its stability to maintain intrinsic S-phase checkpoint functions. J Cell Biol 218:2865-2875 (2019). PubMed: 31366665