Anti-TOM1L2抗体(ab96320)
Key features and details
- Rabbit polyclonal to TOM1L2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TOM1L2抗体
参阅全部 TOM1L2 一抗 -
描述
兔多克隆抗体to TOM1L2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to a region within the internal sequence amino acids 394-457 (
SLAEQRKTVTYEDPQAVGGLASALDNRKQSSEGIPVAQPSVMDDIEVWLR TDLKGDDLEEGVTS
) of Human TOM1L2. -
阳性对照
- A549, HeLa, Jurkat and NT2D1 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96320于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
|
|
| IP |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 56 kDa. |
|
IP
Use a concentration of 5 µg/ml. |
靶标
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功能
Probable role in protein transport. May regulate growth factor-induced mitogenic signaling. -
组织特异性
Ubiquitously expressed with higher expression in heart and skeletal muscle. -
序列相似性
Belongs to the TOM1 family.
Contains 1 GAT domain.
Contains 1 VHS domain. -
结构域
The GAT domain mediates interaction with TOLLIP. - Information by UniProt
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数据库链接
- Entrez Gene: 146691 Human
- Omim: 615519 Human
- SwissProt: Q6ZVM7 Human
- Unigene: 462379 Human
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别名
- FLJ32746 antibody
- Srebf1 antibody
- Target of Myb-like protein 2 antibody
see all
图片
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Immunoprecipitation - Anti-TOM1L2 antibody (ab96320)
TOM1L2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to TOM1L2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96320.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)
>Band: 56kDa; TOM1L2
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Western blot - Anti-TOM1L2 antibody (ab96320)All lanes : Anti-TOM1L2 antibody (ab96320) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : NT2D1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa
7.5% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab96320 被引用在 8 文献中.
- Zakrzewski P et al. Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis†‡. Biol Reprod 102:863-875 (2020). PubMed: 31901088
- Masters TA & Buss F Filopodia formation and endosome clustering induced by mutant plus-end-directed myosin VI. Proc Natl Acad Sci U S A 114:1595-1600 (2017). PubMed: 28143933
- Masters TA et al. MYO6 Regulates Spatial Organization of Signaling Endosomes Driving AKT Activation and Actin Dynamics. Cell Rep 19:2088-2101 (2017). PubMed: 28591580
- Arden SD et al. Loss of cargo binding in the human myosin VI deafness mutant (R1166X) leads to increased actin filament binding. Biochem J 473:3307-19 (2016). WB . PubMed: 27474411
- Tumbarello DA et al. The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy. PLoS Pathog 11:e1005174 (2015). PubMed: 26451915
- Boal F et al. TOM1 is a PI5P effector involved in the regulation of endosomal maturation. J Cell Sci 128:815-27 (2015). PubMed: 25588840
- Shen WC et al. Mutations in the ubiquitin-binding domain of OPTN/optineurin interfere with autophagy-mediated degradation of misfolded proteins by a dominant-negative mechanism. Autophagy 11:685-700 (2015). PubMed: 25484089
- Tumbarello DA et al. Autophagy receptors link myosin VI to autophagosomes to mediate Tom1-dependent autophagosome maturation and fusion with the lysosome. Nat Cell Biol 14:1024-35 (2012). WB, IF . PubMed: 23023224