Application
Western blot
Sample
Human Cell lysate - whole cell (Colorectal cancer, HCT116)
Gel Running Conditions
Reduced Denaturing (10 % seperating gel)
Loading amount
20 µg
Specification
Colorectal cancer, HCT116
Blocking step
gelatin (NET) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: gelatin (NET)
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG Antibody, Peroxidase Conjugat
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: ~75 kDa
Positive control
Overexpression of CTEN with MYC -tag in HCT116 (WT), CTEN fused with MYC-tag and NLS (NLS) and the control used myc-tag only plasmid in HCT116 (EV)
Additional data
Additional Notes
Figure legend: EV: HCT116 cells transfected with control Myc-tag plasmid; WT: HCT116 cells transfected with CTEN fused with Myc-tag plasmid; NLS: HCT116 cells transfected with CTEN fused with Myc-tag plasmid with NLS; Cy: cytoplasmic fraction, Nu: nuclear fraction.
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提交于 Jan 18 2021