重组Anti-TNS4/CTEN抗体[SP83] (ab99887)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP83] to TNS4/CTEN
- Suitable for: IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TNS4/CTEN抗体[SP83]
参阅全部 TNS4/CTEN 一抗 -
描述
兔单克隆抗体[SP83] to TNS4/CTEN -
宿主
Rabbit -
经测试应用
适用于: IHC-P, Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human TNS4/CTEN aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q8IZW8 -
阳性对照
- IHC-P: Human Liver Carcinoma, and Human hepatocellular carcinoma tissue; Flow Cyt (Intra): HeLa.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP83 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99887于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min
|
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
|
说明 |
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IHC-P
1/100. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
May be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton (By similarity). May promote apoptosis, via its cleavage by caspase-3. -
组织特异性
Prostate and placenta. Down regulated in prostate cancer. -
序列相似性
Contains 1 phosphatase tensin-type domain.
Contains 1 SH2 domain. -
翻译后修饰
Proteolytically cleaved by caspase-3 during apoptosis. -
细胞定位
Cell junction > focal adhesion. Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 84951 Human
- Omim: 608385 Human
- SwissProt: Q8IZW8 Human
- Unigene: 438292 Human
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别名
- C terminal tensin like antibody
- C terminal tensin like protein antibody
- C-terminal tensin-like protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling TNS4/CTEN with Purified ab99887 at 1/100 dilution (0.92 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling TNS4/CTEN with purified ab99887 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).
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Staining of Human TNS4/CTEN in a Formalin/PFA-fixed paraffin-embedded sections of Human Liver Carcinoma using ab99887 at a dilution of 1/100.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab99887 被引用在 1 文献中.
- Lu X et al. CTEN Inhibits Tumor Angiogenesis and Growth by Targeting VEGFA Through Down-Regulation of β-Catenin in Breast Cancer. Technol Cancer Res Treat 20:15330338211045506 (2021). PubMed: 34817293