Anti-TNFAIP3抗体(ab193462)
Key features and details
- Rabbit polyclonal to TNFAIP3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TNFAIP3抗体
参阅全部 TNFAIP3 一抗 -
描述
兔多克隆抗体to TNFAIP3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human TNFAIP3 (internal sequence). The exact sequence is proprietary. 17 amino acids.
Database link: P21580 -
阳性对照
- WB: Jurkat cell lysate. IHC-P: Human lung tissue. ICC/IF: Human lung cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab193462于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 89 kDa.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 20 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 89 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 20 µg/ml. |
靶标
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功能
Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. -
序列相似性
Belongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain. -
结构域
The A20-type zinc fingers mediate the ubiquitin ligase activity.
The OTU domain mediates the deubiquitinase activity. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7128 Human
- Omim: 191163 Human
- SwissProt: P21580 Human
- Unigene: 211600 Human
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别名
- A20 antibody
- AISBL antibody
- MGC104522 antibody
see all
图片
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Western Blot of Jurkat cell lysate labeling TNFAIP3 with Anti-TNFAIP3 antibody (ab193462) at (1) 1 and (2) 2µg/ml.
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Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling TNFAIP3 with ab193462 at 5 μg/ml.
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Immunofluorescent analysis of Human lung cells labeling TNFAIP3 with ab193462 at 20 μg/ml.
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab193462 尚未被引用在任何文献中。