Application
Western blot
Sample
Human Cell lysate - whole cell (U937 cells)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin gel)
Loading amount
40 µg
Specification
U937 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody #7076
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
FemtoECL
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 48 kDa
Additional data
Additional Notes
Signal a little lower than expected 52kda. Not sure if specific.
Abcam response
Thank you for submitting your feedback. This antibody is supplied at 0.1 mg/ml, so you might try a dilution of 1/500 or 1/200 to increase the specific signal, and some additional washes to reduce the background. We do of course guarantee this antibody under our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Mar 17 2022