Anti-TNF alpha抗体[52B83] (ab1793)
![Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)](https://www.abcam.cn/ps/products/1/ab1793/Images/TNF-alpha-Primary-antibodies-ab1793-42.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)")
Key features and details
- Mouse monoclonal [52B83] to TNF alpha
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus
- Isotype: IgG1
概述
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产品名称
Anti-TNF alpha抗体[52B83]
参阅全部 TNF alpha 一抗 -
描述
小鼠单克隆抗体[52B83] to TNF alpha -
宿主
Mouse -
特异性
This antibody detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. It reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. It does not react with receptor-bound TNF-alpha. Non-specific background staining is observed in connective tissues. -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus -
免疫原
Full length native protein (purified) (Human).
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阳性对照
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
52B83 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Affibody® Molecule
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Isotype control
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Recombinant Protein
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Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- (R,S)-Rolipram, Selective PDE4 inhibitor (ab120029)
- (S)-(+)-Rolipram, PDE4 inhibitor (ab120030)
- (R)-(-)-Rolipram, Selective PDE4 inhibitor (ab120031)
- Human TNF alpha ELISA Kit (ab181421)
- Mouse TNF alpha ELISA Kit (ab208348)
- Mouse TNF alpha Matched Antibody Pair Kit (ab212073)
- Human TNF alpha Matched Antibody Pair Kit (ab213467)
应用
Our Abpromise guarantee covers the use of ab1793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | 1/10. (methanol fixed cells) ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | Use at an assay dependent concentration. |
靶标
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功能
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. -
疾病相关
Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 7124 Human
- Entrez Gene: 21926 Mouse
- Omim: 191160 Human
- SwissProt: Q8HZD9 Chimpanzee
- SwissProt: P79337 Cynomolgus monkey
- SwissProt: P01375 Human
- SwissProt: P06804 Mouse
- SwissProt: P48094 Rhesus monkey
see all -
别名
- APC1 antibody
- APC1 protein antibody
- Cachectin antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
![Flow Cytometry - Anti-TNF alpha antibody [52B83] (ab1793)](https://www.abcam.cn/ps/products/1/ab1793/Images/TNF-alpha-Primary-antibodies-ab1793-12.jpg)
Flow Cytometry - Anti-TNF alpha antibody [52B83] (ab1793)Overlay histogram showing RAW 264.7 cells stained with ab1793 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1793, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)](https://www.abcam.cn/ps/products/1/ab1793/Images/TNF-alpha-Primary-antibodies-ab1793-41.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)ab1793 staining TNFα in RAW 264.7 cells treated with (R)-(-)-rolipram (ab120031), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R)-(-)-rolipram , as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120031 ((R)-(-)--rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
![Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)](https://www.abcam.cn/ps/products/1/ab1793/Images/TNF-alpha-Primary-antibodies-ab1793-43.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)ab1793 staining TNFα in RAW 264.7 cells treated with (S)-(+)-rolipram (ab120030), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (S)-(+)-rolipram , as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120030 ((S)-(+)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793(5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
文献 (133)
ab1793 被引用在 133 文献中.
- Baya Mdzomba J et al. Nogo-A-targeting antibody promotes visual recovery and inhibits neuroinflammation after retinal injury. Cell Death Dis 11:101 (2020). PubMed: 32029703
- Fallah M et al. Plasminogen is a master regulator and a potential drug candidate for the healing of radiation wounds. Cell Death Dis 11:201 (2020). PubMed: 32205839
- Tu HJ et al. Fibroblast Growth Factor (FGF) Signaling Protects Against Acute Pancreatitis-Induced Damage by Modulating Inflammatory Responses. Med Sci Monit 26:e920684 (2020). PubMed: 32283546
- Tang Z et al. Antitumor Drug Combretastatin-A4 Phosphate Aggravates the Symptoms of Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice. Front Pharmacol 11:339 (2020). PubMed: 32265711
- Zhu H et al. CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-a in TMJ osteoarthritis. Cell Death Dis 11:284 (2020). PubMed: 32332704
