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Immunology Innate Immunity Cytokines TNF Superfamily

Anti-TNF alpha抗体[52B83] (ab1793)

  • Datasheet
  • SDS
Reviews (14)Q&A (33)References (133)

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Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
  • Flow Cytometry - Anti-TNF alpha antibody [52B83] (ab1793)
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)

Key features and details

  • Mouse monoclonal [52B83] to TNF alpha
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-TNF alpha抗体[52B83]
    参阅全部 TNF alpha 一抗
  • 描述

    小鼠单克隆抗体[52B83] to TNF alpha
  • 宿主

    Mouse
  • 特异性

    This antibody detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. It reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. It does not react with receptor-bound TNF-alpha. Non-specific background staining is observed in connective tissues.
  • 经测试应用

    适用于: Flow Cyt, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Apteronotus leptorhynchus
  • 免疫原

    Full length native protein (purified) (Human).

  • 阳性对照

    • 购买相配的WB阳性对照Recombinant Human TNF alpha protein
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    52B83
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    unknown
  • 研究领域

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Cancer
    • Growth factors
    • TNF
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Types of disease
    • Metabolic disorders

相关产品

  • Affibody® Molecule

    • Anti-TNF alpha Affibody® Molecule (ab31908)
    • Anti-TNF alpha Affibody® Molecule (Agarose) (ab31909)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant human TNF alpha protein (Active) (ab259410)
    • Recombinant mouse TNF alpha protein (Active) (ab259411)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • (R,S)-Rolipram, Selective PDE4 inhibitor (ab120029)
    • (S)-(+)-Rolipram, PDE4 inhibitor (ab120030)
    • (R)-(-)-Rolipram, Selective PDE4 inhibitor (ab120031)
    • Human TNF alpha ELISA Kit (ab181421)
    • Mouse TNF alpha ELISA Kit (ab208348)
    • Mouse TNF alpha Matched Antibody Pair Kit (ab212073)
    • Human TNF alpha Matched Antibody Pair Kit (ab213467)

应用

Our Abpromise guarantee covers the use of ab1793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10.

(methanol fixed cells)

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

靶标

  • 功能

    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 疾病相关

    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性

    Belongs to the tumor necrosis factor family.
  • 翻译后修饰

    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 细胞定位

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P01375 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7124 Human
    • Entrez Gene: 21926 Mouse
    • Omim: 191160 Human
    • SwissProt: Q8HZD9 Chimpanzee
    • SwissProt: P79337 Cynomolgus monkey
    • SwissProt: P01375 Human
    • SwissProt: P06804 Mouse
    • SwissProt: P48094 Rhesus monkey
    • Unigene: 241570 Human
    • Unigene: 1293 Mouse
    see all
  • 别名

    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily member 2 antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor (TNF superfamily member 2) antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Flow Cytometry - Anti-TNF alpha antibody [52B83] (ab1793)
    Flow Cytometry - Anti-TNF alpha antibody [52B83] (ab1793)
    Overlay histogram showing RAW 264.7 cells stained with ab1793 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1793, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    ab1793 staining TNFα in RAW 264.7 cells treated with (R)-(-)-rolipram (ab120031), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R)-(-)-rolipram , as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120031 ((R)-(-)--rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)
    Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [52B83] (ab1793)

    ab1793 staining TNFα in RAW 264.7 cells treated with (S)-(+)-rolipram (ab120030), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (S)-(+)-rolipram , as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120030 ((S)-(+)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793(5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

实验方案

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (133)

    发表研究结果有使用 ab1793?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1793 被引用在 133 文献中.

    • Baya Mdzomba J  et al. Nogo-A-targeting antibody promotes visual recovery and inhibits neuroinflammation after retinal injury. Cell Death Dis 11:101 (2020). PubMed: 32029703
    • Fallah M  et al. Plasminogen is a master regulator and a potential drug candidate for the healing of radiation wounds. Cell Death Dis 11:201 (2020). PubMed: 32205839
    • Tu HJ  et al. Fibroblast Growth Factor (FGF) Signaling Protects Against Acute Pancreatitis-Induced Damage by Modulating Inflammatory Responses. Med Sci Monit 26:e920684 (2020). PubMed: 32283546
    • Tang Z  et al. Antitumor Drug Combretastatin-A4 Phosphate Aggravates the Symptoms of Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice. Front Pharmacol 11:339 (2020). PubMed: 32265711
    • Zhu H  et al. CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-a in TMJ osteoarthritis. Cell Death Dis 11:284 (2020). PubMed: 32332704
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 47 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TNF alpha antibody [52B83]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Inflamed palatal gingival tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6 (20min at 95˚C)
    Permeabilization
    No
    Specification
    Inflamed palatal gingival tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Banndith Cheat

    Verified customer

    提交于 Jul 17 2020

    Western blot abreview for Anti-TNF alpha antibody [52B83]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (C42B Prostate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    C42B Prostate cancer cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    提交于 May 29 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TNF alpha antibody [52B83]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (LNCaP C42B prostate cell line)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    LNCaP C42B prostate cell line
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    提交于 Jun 21 2018

    Western blot abreview for Anti-TNF alpha antibody [52B83]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount
    50 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 22 2013

    Question

    Hello,



    I would like to investigate TNF-alpha expression rat perivascular adipose tissue using immunohistochemistry (paraffin sections) and western blotting. Do you know if ab1793 would be suitable to determine expression in rat PVAT or any other white adipose tissue? I haven’t been able to find any specific references in the literature.



    Many thanks

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2012

    Answer

    Thank you for your inquiry.

    I am happy to confirm that ab1793 is tested and guaranteed for rat and WB and IHC-P.

    I can confirm that we do not have any feedback about this antibody in perivascular adipose tissue yet. I therefore suggest running a positive control with a tissue that is known for its expression of TNF alpha.

    I hope this information is helpful and wish you good luck with your experiments.

    Read More

    Abcam Scientific Support

    回复于 Nov 14 2012

    Question

    We see no staining for TNF alpha in sections of frozen PFA-fixed mouse brain and retina.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Oct 26 2012

    Question

    Dear all,
    We had a look and ab1793 and ab18672 seem to look the most suitable. Would you be able to please e-mail him the epitopes of these monoclonals

    Read More

    Abcam community

    Verified customer

    Asked on Sep 11 2012

    Answer

    Thank you for contacting us.

    Unfortunately there is no information available about the epitope recognised by these two antibodies, as it hasn’t been mapped.

    I am very sorry I couldn’t help you further. Please do not hesitate to contact us if you need any other advice or information.

    Read More

    Abcam Scientific Support

    回复于 Sep 11 2012

    Question

    Thank you very much again, I am working on Human cell lines so I thinkab9739 will be good for our work.

    Thank you so much.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 15 2012

    Answer

    Thank you very much for your email.

    My colleague is out of the office today but I will be happy to assist you with this issue.

    I'm sending a free of charge vial of ab9739 on the order ***, which should arrive tomorrow. Please keep us updated about the results with this replacement antibody.

    If you have any questions or need anything else, please let us know and we will be happy to help. Have a nice day.

    Read More

    Abcam Scientific Support

    回复于 Aug 15 2012

    Question

    Thank you very much again for your concern, yes this is our order.
    So, if you can send me another good antibody for TNF-alpha it will be nice.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 14 2012

    Answer

    Thank you for your reply. Are you working in any species or applications other than WB on human samples? We have a large number of antibodies to TNF alpha that are guaranteed for that purpose. For example, our rabbit polyclonal antibodies ab6671 and ab9739 have good customer feedback and have been used in a number of publications. Please let me know which antibody you would like to receive and I will be happy to process your replacement.

    Read More

    Abcam Scientific Support

    回复于 Aug 14 2012

    Question

    Thank you for your reply, and this is my protocol.



    1. I transfected MDA cells in lane 2 and hTERT cells in lane 4 with the TNF-alpha gene. Lane 1:MDA-vector lane4:hTERT vector.

    2. Lysed the cells with Ripa buffer.

    3. 25 ug loading. DTT+loading dye.

    4. Blocking by 5 % dry non fat milk with 1 % BSA in TBST.

    5. Anti-TNF dil 1:1000. 4 C overnight.

    6. Secondary Ab is 1:5000 anti mouse. 1 hr RT.



    but I have no idea about PO number.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 14 2012

    Answer

    Thank you for your reply. Based on the protocol you provided, I would not expect the bands observed on your blot to be specific. I was able to locate an order that was shipped to *** last week for ab1793 and ab15563. Is this the correct order? If I am able to locate your order, I would be happy to offer you a free of charge replacement, credit, or refund. Please let me know how you would like to proceed and I will be happy to help you further.

    Read More

    Abcam Scientific Support

    回复于 Aug 14 2012

    1-10 of 47 Abreviews or Q&A

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